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Quantification of experimental venous thrombus resolution by longitudinal nanogold-enhanced micro-computed tomography.
- Source :
-
Thrombosis Research . Dec2015, Vol. 136 Issue 6, p1285-1290. 6p. - Publication Year :
- 2015
-
Abstract
- Introduction The assessment of thrombus size following treatments directed at preventing thrombosis or enhancing its resolution has generally relied on physical or histological methods. This cross-sectional design imposes the need for increased numbers of animals for experiments. Micro-computed tomography (microCT) has been used to detect the presence of venous thrombus in experimental models but has yet to be used in a quantitative manner. In this study, we investigate the use of contrast-enhanced microCT for the longitudinal assessment of experimental venous thrombus resolution. Materials and methods Thrombi induced by stenosis of the inferior vena cava in mice were imaged by contrast-enhanced microCT at 1, 7 and 14 days post-induction (n = 18). Thrombus volumes were determined longitudinally by segmentation and 3D volume reconstruction of microCT scans and by standard end-point histological analysis at day 14. An additional group of thrombi were analysed solely by histology at 1, 7 and 14 days post-induction (n = 15). Results IVC resident thrombus was readily detectable by contrast-enhanced microCT. MicroCT-derived measurements of thrombus volume correlated well with time-matched histological analyses (ICC = 0.75, P < 0.01). Thrombus volumes measured by microCT were significantly greater than those derived from histological analysis (P < 0.001). Intra- and inter-observer analyses were highly correlated (ICC = 0.99 and 0.91 respectively, P < 0.0001). Further histological analysis revealed noticeable levels of contrast agent extravasation into the thrombus that was associated with the presence of neovascular channels, macrophages and intracellular iron deposits. Conclusion Contrast-enhanced microCT represents a reliable and reproducible method for the longitudinal assessment of venous thrombus resolution providing powerful paired data. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00493848
- Volume :
- 136
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Thrombosis Research
- Publication Type :
- Academic Journal
- Accession number :
- 111410052
- Full Text :
- https://doi.org/10.1016/j.thromres.2015.10.006