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Coumarins with α-glucosidase and α-amylase inhibitory activities from the flower of Edgeworthia gardneri.
- Source :
-
Fitoterapia . Dec2015, Vol. 107, p122-127. 6p. - Publication Year :
- 2015
-
Abstract
- The flower of Edgeworthia gardneri is consumed in beverages in Tibet and has potential health benefits for diabetes. As a part of our continuous studies on dietary supplements for diabetes, two monomers, five dimers and one trimer of coumarins were isolated from the flowers of E. gardneri . One dimer was a new compound ( 1 ) and its structure was determined by spectroscopic methods, including multiple NMR techniques and mass spectrometry. The inhibitory activities of all coumarins against α-amylase and α-glucosidase were evaluated. Compound 4 displayed potent inhibitory effect on both α-amylase and α-glucosidase, with an IC 50 of 90 and 86 μg/mL, respectively. The IC 50 of compound 3 against α-glucosidase was 18.7 μg/mL, and its inhibition mode was noncompetitive. Based on the fluorescence analysis, the binding constant and the number of binding sites of compound 3 were calculated as 2.05 × 10 5 and 1.24, respectively. Furthermore, the interaction between compound 3 and α-glucosidase was a spontaneous process that was driven mainly by hydrophobic force. This study could facilitate the utilization of E gardneri as functional food ingredient. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0367326X
- Volume :
- 107
- Database :
- Academic Search Index
- Journal :
- Fitoterapia
- Publication Type :
- Academic Journal
- Accession number :
- 111296012
- Full Text :
- https://doi.org/10.1016/j.fitote.2015.10.012