Combining immunolabeling and catalyzed reporter deposition to detect intracellular saxitoxin in a cyanobacterium.

We combined the use of polyclonal antibodies against saxitoxin with catalyzed reporter deposition to detect production of saxitoxin by the cyanobacterium Cylindrospermopsis raciborskii . The procedure is simple, allows detection of intracellular saxitoxin in cyanobacteria filaments by confocal laser microscopy and is a promising tool to study toxin production and metabolism. [ABSTRACT FROM AUTHOR]