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Purification, crystallization and preliminary X-ray analysis of a thermostable direct haemolysin from Grimontia hollisae.
- Source :
-
Acta Crystallographica: Section F (Wiley-Blackwell) . Feb2011, Vol. 67 Issue 2, p224-227. 4p. - Publication Year :
- 2011
-
Abstract
- Vibrio hollisae, a halophilic species recently reclassified as Grimontia hollisae, is a causative agent of gastroenteritis and septicaemia. One important pathogenic Vibrio factor, thermostable direct haemolysin (TDH), has been purified and crystallized in two crystal forms using the vapour-diffusion method. The crystals belonged to an orthorhombic space group, with unit-cell parameters a = 104.8, b = 112.4, c = 61.3 Å and a = 122.9, b = 123.3, c = 89.8 Å. The crystals contained either four or eight molecules per asymmetric unit, with predicted solvent contents of 49.4 and 46.3% and Matthews coefficients ( VM) of 2.4 and 2.3 Å3 Da−1, respectively. These crystals were suitable for structure determination, which would yield structural details related to the cytotoxicity and oligomeric structure of this pore-forming toxin. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HALOPHILIC microorganisms
*GASTROENTERITIS
*SEPSIS
*CRYSTALS
*TOXINS
Subjects
Details
- Language :
- English
- ISSN :
- 17443091
- Volume :
- 67
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Acta Crystallographica: Section F (Wiley-Blackwell)
- Publication Type :
- Academic Journal
- Accession number :
- 110812699
- Full Text :
- https://doi.org/10.1107/S1744309110050219