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Screening of the Binding of Small Molecules to Proteins by Desorption Electrospray Ionization Mass Spectrometry Combined with Protein Microarray.

Authors :
Yao, Chenxi
Wang, Tao
Zhang, Buqing
He, Dacheng
Na, Na
Ouyang, Jin
Source :
Journal of the American Society for Mass Spectrometry. Nov2015, Vol. 26 Issue 11, p1950-1958. 9p.
Publication Year :
2015

Abstract

The interaction between bioactive small molecule ligands and proteins is one of the important research areas in proteomics. Herein, a simple and rapid method is established to screen small ligands that bind to proteins. We designed an agarose slide to immobilize different proteins. The protein microarrays were allowed to interact with different small ligands, and after washing, the microarrays were screened by desorption electrospray ionization mass spectrometry (DESI MS). This method can be applied to screen specific protein binding ligands and was shown for seven proteins and 34 known ligands for these proteins. In addition, a high-throughput screening was achieved, with the analysis requiring approximately 4 s for one sample spot. We then applied this method to determine the binding between the important protein matrix metalloproteinase-9 (MMP-9) and 88 small compounds. The molecular docking results confirmed the MS results, demonstrating that this method is suitable for the rapid and accurate screening of ligands binding to proteins. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10440305
Volume :
26
Issue :
11
Database :
Academic Search Index
Journal :
Journal of the American Society for Mass Spectrometry
Publication Type :
Academic Journal
Accession number :
110319497
Full Text :
https://doi.org/10.1007/s13361-015-1221-z