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Hypoxic regulation of plasminogen activator inhibitor-1 expression in human buccal mucosa fibroblasts stimulated with arecoline.
- Source :
-
Journal of Oral Pathology & Medicine . Oct2015, Vol. 44 Issue 9, p669-673. 5p. 1 Color Photograph, 2 Black and White Photographs, 2 Graphs. - Publication Year :
- 2015
-
Abstract
- <bold>Background: </bold>Oral submucous fibrosis (OSF) is regarded as a pre-cancerous condition with fibrosis in oral subepithelial connective tissue. Hypoxia-inducible factor (HIF)-1α regulates a wide variety of profibrogenic genes, which are closely associated with tissue fibrosis. The aim of this study was to compare HIF-1α expression in normal buccal mucosa tissues and OSF specimens and further explore the potential mechanisms that may lead to the induction of HIF-1α expression.<bold>Method: </bold>Twenty-five OSF specimens and six normal buccal mucosa were examined by immunohistochemistry. The expression of HIF-1α from fibroblasts cultured from OSF and normal buccal mucosa was measured by Western blot. Arecoline, a major areca nut alkaloid, was challenged to normal buccal mucosa fibroblasts (BMFs) to elucidate whether HIF-1α expression could affect by arecoline. In addition, the effects of arecoline on plasminogen activator inhibitor (PAI)-1 expression were evaluated in environmental hypoxia.<bold>Results: </bold>HIF-1α expression was significantly higher in OSF specimens and expressed mainly by fibroblasts, epithelial cells, and inflammatory cells. Fibroblasts derived from OSF were found to exhibit higher HIF-1α protein expression than BMFs (P < 0.05). Arecoline was found to upregulate HIF-1α protein in a dose-dependent manner (P < 0.05). Hypoxia increased arecoline-induced PAI-1 protein expression than normoxic conditions (P < 0.05).<bold>Conclusion: </bold>These results suggest that HIF-1α expression is significantly upregulated in OSF tissues from areca quid chewers, implying a potential role as a biomarker for local tissue hypoxia. The activation of HIF-1α may promote fibrogenesis by an increase of PAI-1 expression and a subsequent elevation of extracellular matrix production in oral submucosa leading to fibrosis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FIBROSIS
*ORAL diseases
*PLASMINOGEN activator inhibitors
*PRECANCEROUS conditions
*HYPOXIA-inducible factor 1
*FIBROBLASTS
*PHYSIOLOGY
*BIOCHEMISTRY
*BLOOD coagulation factors
*CELL culture
*CELL physiology
*CONNECTIVE tissues
*EPITHELIAL cells
*IMMUNOHISTOCHEMISTRY
*PHENOMENOLOGY
*ORAL mucosa
*PROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 09042512
- Volume :
- 44
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Journal of Oral Pathology & Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 110119183
- Full Text :
- https://doi.org/10.1111/jop.12284