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Chimeric autologous/allogeneic constructs for skin regeneration.
- Source :
-
Military Medicine . Aug2014 Supplement, Vol. 179 Issue 8, p71-78. 8p. - Publication Year :
- 2014
-
Abstract
- The ideal treatment for severe cutaneous injuries would eliminate the need for autografts and promote fully functional, aesthetically pleasing autologous skin regeneration. NIKS progenitor cell-based skin tissues have been developed to promote healing by providing barrier function and delivering wound healing factors. Independently, a device has recently been created to "copy" skin by harvesting full-thickness microscopic tissue columns (MTCs) in lieu of autografts traditionally harvested as sheets. We evaluated the feasibility of combining these two technologies by embedding MTCs in NIKS-based skin tissues to generate chimeric autologous/allogeneic constructs. Chimeric constructs have the potential to provide immediate wound coverage, eliminate painful donor site wounds, and promote restoration of a pigmented skin tissue possessing hair follicles, sweat glands, and sebaceous glands. After MTC insertion, chimeric constructs and controls were reintroduced into air-interface culture and maintained in vitro for several weeks. Tissue viability, proliferative capacity, and morphology were evaluated after long-term culture. Our results confirmed successful MTC insertion and integration, and demonstrated the feasibility of generating chimeric autologous/allogeneic constructs that preserved the viability, proliferative capacity, and structure of autologous pigmented skin. These feasibility studies established the proof-of-principle necessary to further develop chimeric autologous/allogeneic constructs for the treatment of complex skin defects. [ABSTRACT FROM AUTHOR]
- Subjects :
- *KERATINOCYTES
*AUTOGRAFTS
*CELL culture
*CELL physiology
*COMPARATIVE studies
*HOMOGRAFTS
*RESEARCH methodology
*MEDICAL cooperation
*PLANTS
*REGENERATION (Biology)
*RESEARCH
*SKIN physiology
*SKIN grafting
*WOUND healing
*TISSUE engineering
*EVALUATION research
*PHENOMENOLOGICAL biology
*TRANSPLANTATION of organs, tissues, etc.
Subjects
Details
- Language :
- English
- ISSN :
- 00264075
- Volume :
- 179
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Military Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 109674927
- Full Text :
- https://doi.org/10.7205/MILMED-D-13-00480