Back to Search Start Over

Bacterial cell motility of Burkholderia gut symbiont is required to colonize the insect gut.

Authors :
Lee, Jun Beom
Byeon, Jin Hee
Jang, Ho Am
Kim, Jiyeun Kate
Yoo, Jin Wook
Kikuchi, Yoshitomo
Lee, Bok Luel
Source :
FEBS Letters. Sep2015 Part B, Vol. 589 Issue 19PartB, p2784-2790. 7p.
Publication Year :
2015

Abstract

We generated a Burkholderia mutant, which is deficient of an N -acetylmuramyl- l -alanine amidase, AmiC, involved in peptidoglycan degradation. When non-motile Δ amiC mutant Burkholderia cells harboring chain form were orally administered to Riptortus insects, Δ amiC mutant cells were unable to establish symbiotic association. But, Δ amiC mutant complemented with amiC gene restored in vivo symbiotic association. Δ amiC mutant cultured in minimal medium restored their motility with single-celled morphology. When Δ amiC mutant cells harboring single-celled morphology were administered to the host insect, this mutant established normal symbiotic association, suggesting that bacterial motility is essential for the successful symbiosis between host insect and Burkholderia symbiont. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00145793
Volume :
589
Issue :
19PartB
Database :
Academic Search Index
Journal :
FEBS Letters
Publication Type :
Academic Journal
Accession number :
109395944
Full Text :
https://doi.org/10.1016/j.febslet.2015.08.022