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The caveolin-cavin system plays a conserved and critical role in mechanoprotection of skeletal muscle.
The caveolin-cavin system plays a conserved and critical role in mechanoprotection of skeletal muscle.
- Source :
-
Journal of Cell Biology . 8/31/2015, Vol. 210 Issue 5, p833-849. 17p. - Publication Year :
- 2015
-
Abstract
- Dysfunction of caveolae is involved in human muscle disease, although the underlying molecular mechanisms remain unclear . In this paper, we have functionally characterized mouse and zebraish models of caveolae-associated muscle disease. Using electron tomography, we quantitatively defined the unique three-dimensional membrane architecture of the mature muscle surface. Caveolae occupied around 50% of the sarcolemmal area predominantly assembled into multilobed rosettes. These rosettes were preferentially disassembled in response to increased membrane tension. Caveola-deficient cavin-1-/- muscle fibers showed a striking loss of sarcolemmal organization, aberrant T-tubule structures, and increased sensitivity to membrane tension, which was rescued by muscle-specific Cavin-1 reexpression. In vivo imaging of live zebraish embryos revealed that loss of muscle-specific Cavin-1 or expression of a dystrophy-associated Caveolin-3 mutant both led to sarcolemmal damage but only in response to vigorous muscle activity. Our findings define a conserved and critical role in mechanoprotection for the unique membrane architecture generated by the caveolin-cavin system. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219525
- Volume :
- 210
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- 109335311
- Full Text :
- https://doi.org/10.1083/jcb.201501046