Back to Search Start Over

The caveolin-cavin system plays a conserved and critical role in mechanoprotection of skeletal muscle.

The caveolin-cavin system plays a conserved and critical role in mechanoprotection of skeletal muscle.

Authors :
Lo, Harriet P.
Nixon, Susan J.
Hall, Thomas E.
Cowling, Belinda S.
Ferguson, Charles
Morgan, Garry P.
Schieber, Nicole L.
Fernandez-Rojo, Manuel A.
Bastiani, Michele
Floetenmeyer, Matthias
Martel, Nick
Laporte, Jocelyn
Pilch, Paul F.
Parton, Robert G.
Source :
Journal of Cell Biology. 8/31/2015, Vol. 210 Issue 5, p833-849. 17p.
Publication Year :
2015

Abstract

Dysfunction of caveolae is involved in human muscle disease, although the underlying molecular mechanisms remain unclear . In this paper, we have functionally characterized mouse and zebraish models of caveolae-associated muscle disease. Using electron tomography, we quantitatively defined the unique three-dimensional membrane architecture of the mature muscle surface. Caveolae occupied around 50% of the sarcolemmal area predominantly assembled into multilobed rosettes. These rosettes were preferentially disassembled in response to increased membrane tension. Caveola-deficient cavin-1-/- muscle fibers showed a striking loss of sarcolemmal organization, aberrant T-tubule structures, and increased sensitivity to membrane tension, which was rescued by muscle-specific Cavin-1 reexpression. In vivo imaging of live zebraish embryos revealed that loss of muscle-specific Cavin-1 or expression of a dystrophy-associated Caveolin-3 mutant both led to sarcolemmal damage but only in response to vigorous muscle activity. Our findings define a conserved and critical role in mechanoprotection for the unique membrane architecture generated by the caveolin-cavin system. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219525
Volume :
210
Issue :
5
Database :
Academic Search Index
Journal :
Journal of Cell Biology
Publication Type :
Academic Journal
Accession number :
109335311
Full Text :
https://doi.org/10.1083/jcb.201501046