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SARS-coronavirus replicates in mononuclear cells of peripheral blood (PBMCs) from SARS patients
- Source :
-
Journal of Clinical Virology . Dec2003, Vol. 28 Issue 3, p239. 6p. - Publication Year :
- 2003
-
Abstract
- Background: The etiologic agent of severe acute respiratory syndrome (SARS) is a recently identified, positive single-stranded RNA (ssRNA) coronavirus (SARS-CoV). Little is known about the dynamic changes of the viral replicative form in SARS cases. Objectives: Evaluate whether SARS-CoV can infect and replicate in peripheral blood mononuclear cells (PBMCs) of infected persons and reveal any dynamic changes to the virus during the course of the disease. Study design: Peripheral blood mononuclear cells collected from SARS cases infected by the same infectious source were tested for both negative-stranded RNA (minus-RNA, “replicative intermediates”) and positive-stranded RNA (genomic RNA) of SARS-CoV during the course of hospitalization by reverse transcription-polymerase chain reaction (RT-PCR). Results: SARS-CoV minus-RNA was detected in PBMCs from SARS patients. The viral replicative forms in PBMCs were detectable during a period of 6 days post-onset of the disease, while the plus-RNA were detectable for a longer period (8–12 days post-onset). Conclusions: SARS-coronavirus can infect and replicate within PBMCs of SARS patients, but viral replication in PBMCs seems subject to self-limitation. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 13866532
- Volume :
- 28
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Clinical Virology
- Publication Type :
- Academic Journal
- Accession number :
- 10926890
- Full Text :
- https://doi.org/10.1016/S1386-6532(03)00195-1