Back to Search
Start Over
Hepatoprotective Effect of Terminalia chebula against t-BHP-Induced Acute Liver Injury in C57/BL6 Mice.
- Source :
-
Evidence-based Complementary & Alternative Medicine (eCAM) . 2015, p1-11. 11p. - Publication Year :
- 2015
-
Abstract
- We aimed to identify the hepatoprotective effects of Terminalia chebula water extract (TCW) and its corresponding pharmacological actions using C57/BL6 mice model of tert-butylhydroperoxide-(t-BHP-) induced acute liver injury. Mice were orally administered with TCW (0, 50, 100, or 200mg/kg) or gallic acid (100mg/kg) for 5 days before t-BHP (2.5mM/kg) injection. Liver enzymes, histopathology, oxidative stress parameters, antioxidant components, and inflammatory cytokines were examined 18 h after t-BHP injection. t-BHP injection caused dramatic elevation of serum AST, ALT, and LDH level, while TCW pretreatment notably attenuated these elevations. Inflammatory cytokines including TNF-α, IL-1β, and IL-6 were notably increased in hepatic tissues, and then these were efficiently attenuated by TCW pretreatment. t-BHP injection notably increased malondialdehyde, total reactive oxygen species, and nitric oxide in the liver tissue, while it markedly dropped the antioxidant activities including total antioxidant capacity, total glutathione contents, glutathione peroxidase, superoxide dismutase, and catalase. TCW pretreatment remarkably ameliorated these alterations, and these effects were relevant to gene expressions. Histopathological examinations supported the above findings. Collectively, these findings well prove that TCW beneficially prevents acute and severe liver injury and clarify its corresponding mechanisms involved in the inhibition of oxidative stress and inflammatory cytokines. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HEPATOTOXICOLOGY
*GENES
*LIVER analysis
*ENZYME metabolism
*REACTIVE oxygen species
*ALTERNATIVE medicine
*ANIMAL experimentation
*ANTIOXIDANTS
*ASPARTATE aminotransferase
*BIOPHYSICS
*PHYSICAL & theoretical chemistry
*CYTOKINES
*GLUTATHIONE
*HIGH performance liquid chromatography
*HISTOLOGICAL techniques
*IMMUNOHISTOCHEMISTRY
*INTERLEUKINS
*LACTATE dehydrogenase
*MASS spectrometry
*RESEARCH methodology
*MEDICINAL plants
*LIPID peroxidation (Biology)
*MICE
*NITRIC oxide
*ORAL drug administration
*POLYMERASE chain reaction
*PROBABILITY theory
*RESEARCH funding
*STATISTICS
*SUPEROXIDE dismutase
*TUMOR necrosis factors
*MALONDIALDEHYDE
*PLANT extracts
*DATA analysis
*OXIDATIVE stress
*ALANINE aminotransferase
*DESCRIPTIVE statistics
*ONE-way analysis of variance
*PREVENTION
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Academic Search Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 108824735
- Full Text :
- https://doi.org/10.1155/2015/517350