First record of a Chironomidae pupa in amber.

A pupal exuviae of a non-biting midge (Chironomidae) is recorded in Eocene Rovno amber from Ukraine. The exuviae belongs to an Orthocladiinae species. It possesses several traits characteristic for terrestrial species including non-fringed anal lobes without macrosetae, tergites with only minute shagreen, bare conjunctives, and absence of thoracic horns and frontal warts. Up to now, this is the earliest record of a preimaginal stage of Orthocladiinae, and the only record of a chironomidae pupal exuviae from amber. [ABSTRACT FROM AUTHOR]