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In vitro Development of Murine Embryos in presence of Campylobacter fetus.

In vitro Development of Murine Embryos in presence of Campylobacter fetus.

Authors :
Catena, M.
Teruel, M.
Morán, P.
Chiapparrone, M.
Echevarria, H.
Soto, P.
Source :
International Journal of Morphology. 2013, Vol. 31 Issue 4, p1168-1174. 7p.
Publication Year :
2013

Abstract

Bovine campylobacteriosis caused by Campylobacter fetus is associated with reproductive losses. The knowledge about the mechanisms of bacterial pathogenesis is limited, then a murine experimental model is proposed. BALB/c females and males were used. Two-cell embryos were cultured in Ham-F10 as control group (CG). Treatment groups were constituted by the addition of Cfv 1 and 3, or Cff 2 and 5. Morulae were placed in Ham-F10 (CG); treatment groups were constituted by the addition of Cfv27, CFF (cell- free filtrate) and Brucella broth (BB). Blastocysts were cultured in MEM (CG); challenge group were constituted by the addition of Cfv 27. Differentiation, hatching, hatched, adhesion and expansion were evaluated. Results were analyzed by Chi² test. In two-cell embryo, the differentiation rate was not modified when the study strains were added (p > 0.05). The differentiation rate at 24 h for embryos at the morula stage was lower for BB, Cfv, and CFF, compared with CG (p < 0.05). After 48 h culture, no differences were observed in blastocyst formation for Cfv and BB, compared to CG (p > 0.05). However, the differentiation rate for the CFF group was lower than for CG (p < 0.05). At 48 and 72 h, the hatching rate was higher in CFF and Cfv groups than in CG (p < 0.05). Differences were not detected in blastocyst cultures. In conclusion, under these experimental conditions, Cf was not detrimental to the development of murine embryos. Efforts will be intensified to establish in vitro infection models that reproduce their pathogenicity. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
07179367
Volume :
31
Issue :
4
Database :
Academic Search Index
Journal :
International Journal of Morphology
Publication Type :
Academic Journal
Accession number :
108747830
Full Text :
https://doi.org/10.4067/S0717-95022013000400004