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The murine double-stranded RNA-dependent protein kinase PKR and the murine 2′,5′-oligoadenylate synthetase-dependent RNase L are required for IFN-β-mediated resistance against herpes simplex virus type 1 in primary trigeminal ganglion culture
- Source :
-
Virology . Aug2003, Vol. 313 Issue 1, p126. 10p. - Publication Year :
- 2003
-
Abstract
- A study was undertaken to evaluate the efficacy of an adenoviral construct expressing the murine interferon-β (IFN-β) transgene (Ad:IFN-β) against herpes simplex virus type 1 (HSV-1) infection in a primary trigeminal ganglion (TG) cell culture. The transduction efficiency ranged from 0.2 to 11.0% depending on the multiplicity of infection (m.o.i.) of the adenoviral vector (0.5–50.0). Moreover, neurons were the main target of the adenoviral transduction. TG cultures transduced with Ad:IFN-β displayed up to a 19-fold reduction in viral titers compared with cells transduced with an Ad:Null or nontransduced TG culture controls. Transduction with Ad:IFN-β up-regulated two critical antiviral genes, double-stranded RNA-dependent protein kinase R (PKR) and 2′,5′-oligoadenylate synthetase (OAS). The absence of PKR or RNase L (downstream effector molecule of OAS) attenuated Ad:IFN-β efficacy against HSV-1 replication, implicating a critical role for PKR and OAS/RNase systems in the establishment of IFN-induced resistance against HSV-1 in TG cells. [Copyright &y& Elsevier]
- Subjects :
- *HERPES simplex virus
*BACTERIOPHAGES
*DOUBLE-stranded RNA
*PROTEIN kinases
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Volume :
- 313
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 10697411
- Full Text :
- https://doi.org/10.1016/S0042-6822(03)00298-8