A Story of Chelatase Evolution.

Authors :: Brindley, Amanda A.
Raux, Evelyne
Leech, Helen K.
Schubert, Heidi L.
Warren, Martin J.
Source :: Journal of Biological Chemistry. 6/20/2003, Vol. 278 Issue 25, p22388. 8p. 3 Diagrams, 3 Charts, 4 Graphs.
Publication Year :: 2003
Abstract: Describes the cloning of the cobalamin (CbiX) gene from two methanogens, the overproduction of CbiX as a recombinant protein, and its inherent enzymatic activity in order to demonstrate that CbiX represents a compact cobalt chelatase in methanogens. Protein expression; Structural topology predictions for the CbiX[supS] and CbiX[supL] enzymes.