Ubiquitin Conjugation Is Not Required for the Degradation of Oxidized Proteins by Proteasome.

Determines whether ubiquitin conjugation is necessary for the degradation of oxidized proteins in intact cells. Inhibition of degradation by proteasome inhibitors; Rates of oxidized protein degradation by cell lysates; Presence of adenosine triphosphate; Tendency of a protein to be ubiquitinylated.