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Rapid and sensitive analysis of cyclobenzaprine by LC-MS/MS: Application to a pharmacokinetic study of cyclobenzaprine in dog.
- Source :
-
Asian Journal of Pharmaceutical Sciences . 2014, Vol. 9 Issue 2, p117-122. 6p. - Publication Year :
- 2014
-
Abstract
- A rapid and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the quantification of cyclobenzaprine in dog plasma. After extracted with organic solvent, post-treatment samples were separated on an Agela C18 column interfaced with a triple quadrupole tandem mass spectrometer in positive electrospray ionization mode. Multiple reaction monitoring was performed using the transitions of m/z 276.2 → 216.1 and m/z 325.1 → 109.0 to quantify cyclobenzaprine and escitalopram (internal standard), respectively. The mobile phase consisted of acetonitrile: 5 mM ammonium acetate: formic acid (90:10:0.01, v/v/v) at a flow rate of 0.3 ml/min. The total analysis time was 2.4 min. The method was linear over the concentration range of 0.0200-10.0 ng/ml. The intra- and inter-day precision was within 12.8% in terms of relative standard deviation (RSD%) and the accuracy within 5.6% in terms of relative error. This method was successfully applied in a pharmacokinetic study of extended-release cyclobenzaprine in dogs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 18180876
- Volume :
- 9
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Asian Journal of Pharmaceutical Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 103682161
- Full Text :
- https://doi.org/10.1016/j.ajps.2014.01.002