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Simultaneous Quantitation of Na+ and K+ in Single Normal and Cancer Cells Using a New Near-Infrared Fluorescent Probe.
- Source :
-
Analytical Chemistry . 6/16/2015, Vol. 87 Issue 12, p6057-6063. 7p. - Publication Year :
- 2015
-
Abstract
- Considering the important functions of cellular Na+ and K+ together with their cooperative efforts on various biological processes, it is significant to simultaneously detect Na+ and K+ at a single-cell level. Here, we present a novel method to discriminate and quantify simultaneously Na+ and K+ in single cells using a new near-infrared fluorescent probe associated with microchip electrophoresis. The fluorescent probe selectively responds to both Na+ and K+. The microchip electrophoresis allows accurate single-cell manipulation and effective distinction of Na+ and K+. Based on the method, the concentration of Na+ and K+ in single normal and cancer cells was compared, and the variation of Na+ and K+ in single cancer cells during the early stage of apoptotic volume decrease was monitored, which would help us to better understand the critical roles of Na+ and K+ in malignant cells and apoptosis. This method has paved a new way for the research of the synergistic function of Na+ and K+ in the regulation of various biological processes at a single-cell level. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 87
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 103629122
- Full Text :
- https://doi.org/10.1021/acs.analchem.5b00571