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The Mitochondrial Antiviral Protein MAVS Associates with NLRP3 and Regulates Its Inflammasome Activity.

Authors :
Sangjun Park
Juliana, Christine
Sujeong Hong
Datta, Pinaki
Inhwa Hwang
Fernandes-Alnemri, Teresa
Je-Wook Yu
Alnemri, Emad S.
Source :
Journal of Immunology. 10/15/2013, Vol. 191 Issue 8, p4358-4366. 9p.
Publication Year :
2013

Abstract

NLRP3 assembles an inflammasome complex that activates caspase-1 upon sensing various danger signals derived from pathogenic infection, tissue damage, and environmental toxins. How NLRP3 senses these various stimuli is still poorly understood, but mitochondria and mitochondrial reactive oxygen species have been proposed to play a critical role in NLRP3 activation. In this article, we provide evidence that the mitochondrial antiviral signaling protein MAVS associates with NLRP3 and facilitates its oligomerization leading to caspase-1 activation. In reconstituted 293T cells, full-length MAVS promoted NLRP3-dependent caspase-1 activation, whereas a C-terminal transmembrane domain-truncated mutant of MAVS (MAVS-ΔTM) did not. MAVS, but not MAVSΔTM, interacted with NLRP3 and triggered the oligomerization of NLRP3, suggesting that mitochondrial localization of MAVS and intact MAVS signaling are essential for activating the NLRP3 inflammasome. Supporting this, activation of MAVS signaling by Sendai virus infection promoted NLRP3-dependent caspase-1 activation, whereas knocking down MAVS expression clearly attenuated the activation of NLRP3 inflammasome by Sendai virus in THP-1 and mouse macrophages. Taken together, our results suggest that MAVS facilitates the recruitment of NLRP3 to the mitochondria and may enhance its oligomerization and activation by bringing it in close proximity to mitochondrial reactive oxygen species. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00221767
Volume :
191
Issue :
8
Database :
Academic Search Index
Journal :
Journal of Immunology
Publication Type :
Academic Journal
Accession number :
103555646
Full Text :
https://doi.org/10.4049/jimmunol.1301170