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Functional analysis of microRNA activity in Brugia malayi.
- Source :
-
International Journal for Parasitology . Aug2015, Vol. 45 Issue 9/10, p579-583. 5p. - Publication Year :
- 2015
-
Abstract
- The complement of the Brugia malayi microRNA-71 was inserted into the 3′ untranslated region of a reporter plasmid, resulting in a decrease in reporter activity. Mutation of the seed sequence restored activity. Insertion of the 3′ untranslated regions from two algorithm-predicted putative target genes into the reporter resulted in a similar decrease in activity; mutation of the predicted target sequences restored activity. These experiments demonstrate that B. malayi microRNA targets may be predicted using current algorithms and describe a functional assay to confirm predicted targets. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICRORNA
*BRUGIA
*SPIRURIDA
*BRUGIA malayi
*NUCLEIC acids
Subjects
Details
- Language :
- English
- ISSN :
- 00207519
- Volume :
- 45
- Issue :
- 9/10
- Database :
- Academic Search Index
- Journal :
- International Journal for Parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 103552835
- Full Text :
- https://doi.org/10.1016/j.ijpara.2015.04.004