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Development of a liquid chromatography with mass spectrometry method for the determination of gelsemine in rat plasma and tissue: Application to a pharmacokinetic and tissue distribution study.

Authors :
Zhang, Shuangshuang
Hu, Shuping
Yang, Xiangxiang
Shen, Jiaqi
Zheng, Xiaoyong
Huang, Kexin
Xiang, Zheng
Source :
Journal of Separation Science. Mar2015, Vol. 38 Issue 6, p936-942. 7p.
Publication Year :
2015

Abstract

Gelsemine from Gelsemium elegans Benth is a potential anesthetic and analgesic agent with no physical dependence and opiate addiction. This study was aimed at developing an ultrafast liquid chromatography coupled to tandemmass spectrometrymethod to quantify gelsemine in rat plasma and tissues. Plasma and tissues were processed with acetonitrile precipitation, and dendrobine was chosen as the internal standard. Sample separation was performed on an ACQUITY HSS T3 column. The mobile phase consisted of acetonitrile and 0.1% formic acid aqueous solution. Multiple reactions monitoring mode was utilized to detect the compounds of interest. The mass spectrometer was operated in the positive ion mode for detection. The MS/MS ion transitions monitored were m/z 323.2→70.5 for gelsemine and 264.2→108.05 for dendrobine, respectively. The calibration curves were linear over the range of 1–500 ng/mL in all biological matrices. The lower limit of quantification for rats plasma and tissues was 1.0 ng/mL. The values for inter- and intraday precision and accuracy were well within the ranges acceptable (< 15%). It was successfully applied to the pharmacokinetic and tissue distribution studies of gelsemine after intravenous doses of 5, 2, and 0.5 mg/kg in rats. These data of gelsemine would be useful for clinical application and further development. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16159306
Volume :
38
Issue :
6
Database :
Academic Search Index
Journal :
Journal of Separation Science
Publication Type :
Academic Journal
Accession number :
103277151
Full Text :
https://doi.org/10.1002/jssc.201401168