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Piscroside C, a novel iridoid glycoside isolated from Pseudolysimachion rotundum var. subinegrum suppresses airway inflammation induced by cigarette smoke.
- Source :
-
Journal of Ethnopharmacology . Jul2015, Vol. 170, p20-27. 8p. - Publication Year :
- 2015
-
Abstract
- Ethnopharmacological relevance Pseudolysimachion rotundum var . subintegrum (Speedwell, Plantaginaceae) is used as a traditional herbal medicine for treating bronchitis, cough and asthma in Korea, China, Russia, and Europe. Aim of the study In this study, we investigated the protective effects of the novel iridoid glycoside, piscroside C (compound 1) isolated from the methanolic extract of P. rotundum var. subintegrum against inflammatory responses using a cigarette smoke induced chronic obstructive pulmonary disease (COPD) and TNF-α-stimulated human airway epithelial NCI-H292 cells. Materials and methods The novel iridoid glycoside piscroside C was isolated from the methanolic extract of P. rotundum var. subintegrum . The chemical structure was established by NMR, HRESIMS, and optical rotation. In in vivo experiment, the mice received 1 h of cigarette smoke for 3 days. Piscroside C was administered to mice by oral gavage 1 h before cigarette smoke exposure for 3 days. In in vitro experiment, we evaluated the effect of piscroside C on proinflammatory mediators in H292 cells stimulated with TNF-α. Results Piscroside C significantly reduced the neutrophil influx, reactive oxygen species production, IL-6, TNF-α, and elastase activity in bronchoalveolar lavage fluid in COPD animals. In addition, piscroside C attenuated NF-κB and IκB phosphorylation, leading to reduced recruitment of inflammatory cells into the lung tissue. Consistent with the results of in vivo experiment, piscroside C significantly inhibited the expression of inflammatory cytokines (IL-6, IL-8 and IL-1β) by inhibiting NF-κB activation, as resulting decrease in the phosphorylation of IKKβ, IκBα and TAK1 in TNF-α-stimulated H292 cells. Conclusion These findings indicate that piscroside C effectively inhibits inflammatory responses, which is an important process in the development of COPD through suppression of IKK/NF-κB activation. Our study suggest that piscroside C might represent a useful therapeutic for the treatment of inflammatory airway disease. [ABSTRACT FROM AUTHOR]
- Subjects :
- *OBSTRUCTIVE lung diseases
*LUNG analysis
*REACTIVE oxygen species
*ALTERNATIVE medicine
*ANIMAL experimentation
*ANTI-inflammatory agents
*BIOLOGICAL models
*BIOPHYSICS
*BRONCHOALVEOLAR lavage
*CYTOKINES
*GLYCOSIDES
*HISTOLOGICAL techniques
*INTERLEUKINS
*LUNGS
*RESEARCH methodology
*MEDICINAL plants
*MICE
*NEUTROPHILS
*ORAL drug administration
*PASSIVE smoking
*PHOSPHORYLATION
*TUMOR necrosis factors
*DNA-binding proteins
*PLANT extracts
*STATISTICAL significance
*DESCRIPTIVE statistics
*IN vitro studies
*PHARMACODYNAMICS
*PREVENTION
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 170
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 103236286
- Full Text :
- https://doi.org/10.1016/j.jep.2015.04.043