Protamine expression in severe teratozoospermia patients.

Objective To investigate the difference of content and expression of protamine (TNBP) in the severe teratozoospermia patients and provide the theoretical basis. Methods It was extracted TNBP of semen protamine from 23 severe teratozoospermia patients(the severe teratozoospermia group) and 37 healthy men(the control group) from Feruary 2013 to May 2014 with polyacrylamide gelelectrophoresis and then confirm the relative content of proein bands scanned by micro imaging density measuring instrument under the cataphoresis in the polyacrylamide hydrogel. Results The value of TH/TP, TNBP1/TNBP2, TP/TNBP2 in the evere teratozoospermia group were significantly higher than those of the control group (P<0.05). Compared with the control group, there was no significant difference in TNBP1/TP value (P>0.05). Thesperm density was lower than that of the control group (P<0.05). Both the sperm survival rate and the precession movement rate showed no significant difference with the control group (P>0.05). Conclusion The abnormity of TNBP1/TNBP2,TP/TNBP2 in the relative content of TNBP is one of the reasons leading to severe teratozoospermia. [ABSTRACT FROM AUTHOR]