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Characterization of bovine serum albumin partitioning behaviors in polymer-salt aqueous two-phase systems.
- Source :
-
Journal of Bioscience & Bioengineering . Jul2015, Vol. 120 Issue 1, p85-90. 6p. - Publication Year :
- 2015
-
Abstract
- In this paper, a linear relationship is proposed relating the natural logarithm of partition coefficient, ln K for protein partitioning in poly (ethylene glycol) (PEG)-phosphate aqueous two-phase system (ATPS) to the square of tie-line length ( TLL 2 ). This relationship provides good fits ( r 2 > 0.98) to the partition of bovine serum albumin (BSA) in PEG (1450 g/mol, 2000 g/mol, 3350 g/mol, and 4000 g/mol)-phosphate ATPS with TLL of 25.0–50.0% (w/w) at pH 7.0. Results also showed that the plot of ln K against pH for BSA partitioning in the ATPS containing 33.0% (w/w) PEG1450 and 8.0% (w/w) phosphate with varied working pH between 6.0 and 9.0 exhibited a linear relationship which is in good agreement ( r 2 = 0.94) with the proposed relationship, ln K = α′ pH + β′ . These results suggested that both the relationships proposed could be applied to correlate and elucidate the partition behavior of biomolecules in the polymer-salt ATPS. The influence of other system parameters on the partition behavior of BSA was also investigated. An optimum BSA yield of 90.80% in the top phase and K of 2.40 was achieved in an ATPS constituted with 33.0% (w/w) PEG 1450 and 8.0% (w/w) phosphate in the presence of 8.5% (w/w) sodium chloride (NaCl) at pH 9.0 for 0.3% (w/w) BSA load. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13891723
- Volume :
- 120
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Bioscience & Bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 102982985
- Full Text :
- https://doi.org/10.1016/j.jbiosc.2014.11.021