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Cholesterol under oxidative stress—How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols.

Authors :
Kulig, Waldemar
Olżyńska, Agnieszka
Jurkiewicz, Piotr
Kantola, Anu M.
Komulainen, Sanna
Manna, Moutusi
Pourmousa, Mohsen
Vazdar, Mario
Cwiklik, Lukasz
Rog, Tomasz
Khelashvili, George
Harries, Daniel
Telkki, Ville-Veikko
Hof, Martin
Vattulainen, Ilpo
Jungwirth, Pavel
Source :
Free Radical Biology & Medicine. Jul2015, Vol. 84, p30-41. 12p.
Publication Year :
2015

Abstract

The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized—tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
08915849
Volume :
84
Database :
Academic Search Index
Journal :
Free Radical Biology & Medicine
Publication Type :
Academic Journal
Accession number :
102982275
Full Text :
https://doi.org/10.1016/j.freeradbiomed.2015.03.006