Decolorization on Indigo Dyeing Wastewater by Laccase from Coriolus versicolor.

[Object] The study aimed to discuss the decolorization on indigo dyeing wastewater by laccase from Coriolus versicolor. [Method] Firstly, the effects of temperature, pH, indigo concentration, HBT concentration, laccase dosage on the decolorization of indigo dyeing wastewater by laccase /HBT, and then the synergism of laccase and acid cellulase was analyzed. [Result] Using ABTS as the substrate, the kinetic parameters, Km and Vmax, were 0. 318 mmol/L and 0. 035 5 mmol/(L·min) respectively. The decolorization rate of indigo reached 96. 5% when the laccase acted on indigo for 40 min with HBT as an introducer at temperature 50 °C, pH =4. 5, indigo concentration 100 mg /L, HBT concentration 0. 1% and laccase dosage 100 IU /L. Due to the synergism of laccase and acid cellulase during the bio-finishing of blue jeans, the backstaining degree of blue jeans reduced by 85% when the amount of laccase added was 15 000 IU /kg. Menawhile, the synergism of the laccase and acid cellulase decreased indigo concentration in wastewater by 83. 8%. [Conclusion] The laccase from Coriolus versicolor had a good prospect in the bio-finishing of blue jeans and the decolorization of indigo dyeing wastewater. [ABSTRACT FROM AUTHOR]