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Simultaneous determination of glycine betaine and arsenobetaine in biological samples by HPLC/ICPMS/ESMS and the application to some marine and freshwater fish samples.
- Source :
-
Microchemical Journal . Sep2015, Vol. 122, p172-175. 4p. - Publication Year :
- 2015
-
Abstract
- We describe a HPLC/mass spectrometry method for the simultaneous determination of glycine betaine and arsenobetaine in biological samples. The sample preparation included a water/methanol extraction followed by clean-up of extracts on a strong cation-exchange resin; the HPLC system consisted of a cation-exchange column with an ammonium formate buffer solution as mobile phase. Glycine betaine and arsenobetaine were quantified in a single chromatographic run by splitting the HPLC flow with an adjustable flow splitter and detecting glycine betaine selectively by electrospray MS in the positive single ion monitoring mode at m/z 118, and arsenobetaine with the arsenic-selective detector ICPMS at m/z 75. The proposed method was validated for arsenobetaine by analysis of CRM Dorm-2, and for glycine betaine by spiking the CRM Dorm-2 with a defined amount of glycine betaine. Finally, the developed method was applied to determine glycine betaine/arsenobetaine ratios in single specimens of four species of marine fish and one species of freshwater fish. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0026265X
- Volume :
- 122
- Database :
- Academic Search Index
- Journal :
- Microchemical Journal
- Publication Type :
- Academic Journal
- Accession number :
- 102920122
- Full Text :
- https://doi.org/10.1016/j.microc.2015.04.022