LvCTL3 原核表达及其对刺激隐核虫的凝集作用研究.

Cryptocaryon irritans Brown is an important pathogen related to sea-fish, the study on invasion mech blocker showed great significance. In this paper, c-type lectin LvCTL3 from shrimp Litopenaeus Vanname was cloned and expressed by genet ic engineering methods, and then the recombinant protein was used to agglutination test with Cryptocaryon irritans Broon including Protomont and Theront. The results show that both Protomont and Theront has good aggregation effect with recombinant protein r-LvCTL3, furthermore, the vitality of Theront was restrain obviously. The result mean that rich of glycoprotein molecules, which may be diverse, distribute on the surface of Protomont and Theront, resulted in aggregation effect with r-LvCTL3 . [ABSTRACT FROM AUTHOR]