Inhibiting effect of alcohol extract from Dioscore bulbifera on gastric cancer cells.

Objective To investigate the inhibiting effects of alcohol extract from Dioscore bulbifera on proliferation, colony formation and migration of cancer cell lines. Methods Alcohol extract from Dioscore bulbifera was prepared using Soxhlet extraction. Human gastric cancer cell line MGC803 was treated with different concentrations (0, 60, 120 mg/L) of alcohol extract from Dioscore bulbifera. In vitro, proliferation, colony formation and migration of gastric cancer cells were detected by MTT, colony formation experiments and Transwell assay respectively. Results The proliferation (day 2-day 6, F=29.130, 21.864, 67.826, 36.015, 43.656, P < 0.01)and colony formation (F=11.918, P < 0.01)of gastric cancer cells were significantly inhibited by administration of alcohol extract from Dioscore bulbifera at both 60 mg/L and 120 mg/L . The migration (F=4.258, P <0.05)of gastric cancer cells were significantly suppressed after cells were treated with 120 mg/L alcohol extract from Dioscore bulbifera. Conclusion Alcohol extract from Dioscore bulbifera significantly inhibit proliferation, colony formation and migration of gastric cancer cells. [ABSTRACT FROM AUTHOR]