Application of novel magnetizable cellulose particles in the development of immunoradiometric assay for C-peptide.

The present study was oriented to develop, optimize and validate immunoradiometric assay for the measurement of C-peptide in human serum by employing magnetizable cellulose particles prepared by a novel method of wet grinding. Six commercial monoclonal antibodies raised against the different epitopes of C-peptide were evaluated for their compatibility as sandwich partners by 'cross matching'. The developed one step inclusive assay with best matched combination of sandwich complex, 'magnetizable cellulose particles linked capture antibody-C-peptide-I labelled detector antibody' had an analytical sensitivity of 0.16 ng/ml and standard range of 0.3-30 ng/ml covering required physiological and diagnostic range. [ABSTRACT FROM AUTHOR]