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BRCA 1 mutation site may be linked with nuclear DNA ploidy in BRCA 1-mutated ovarian carcinomas.
- Source :
-
Asia Pacific Journal of Clinical Oncology . Jun2015, Vol. 11 Issue 2, p135-141. 7p. - Publication Year :
- 2015
-
Abstract
- Aims BRCA1 has a role in maintaining normal nuclear DNA content during cell division and its inactivation may result in DNA aneuploidy and cancer progression. BRCA 1-linked breast cancers are more aneuploid and have a worse prognosis, but this has not been elucidated in ovarian cancers. This study explores the potential difference in ploidy status between BRCA 1-mutated and sporadic ovarian carcinomas. It also explores the potential association between BRCA 1 mutation site and DNA ploidy status. Methods This study compared DNA ploidy status of tumor blocks from 23 BRCA 1-mutated ovarian carcinomas with that of 23 sporadic ovarian carcinomas matched for histologic subtype, patient age, stage and grade. DNA content of the nuclei was measured by Feulgen- Schiff staining followed by image cytometry and compared. Results BRCA 1-linked tumors with a stop codon closer to the N-terminal (between 1 and 500 aa; 6/6, 100%) had a significantly higher frequency of nondiploidy compared with those with stop codon above 500 aa (7/12, 58%) ( P = 0.033). A diploid peak was detected in 28% of BRCA 1-mutated ovarian cancers and in 33% of sporadic ovarian cancers. Conclusions The present study concluded that ovarian tumors with mutations closer to the N-terminal of BRCA 1 may have a higher risk of DNA aneuploidy. There is no significant difference between BRCA 1-mutated and sporadic ovarian carcinomas with respect to the DNA content. [ABSTRACT FROM AUTHOR]
- Subjects :
- *OVARIAN cancer
*BRCA genes
*GENETIC mutation
*DNA
*PLOIDY
*CELL division
Subjects
Details
- Language :
- English
- ISSN :
- 17437555
- Volume :
- 11
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Asia Pacific Journal of Clinical Oncology
- Publication Type :
- Academic Journal
- Accession number :
- 102481836
- Full Text :
- https://doi.org/10.1111/ajco.12310