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Bifunctional Glass Membrane Designed to Interface SDS-PAGE Separations of Proteins with the Detection of Peptides by Mass Spectrometry.
- Source :
-
Analytical Chemistry . 4/7/2015, Vol. 87 Issue 7, p3685-3693. 9p. - Publication Year :
- 2015
-
Abstract
- We describe the construction and characterization of a novel membrane designed to allow proteins separated by gel electrophoresis (SDS-PAGE) to be detected as peptides by mass spectrometry in an efficient and comprehensive manner. The key attribute of the membrane is a bifunctional design that allows for the digestion of protein(s) and retention of the resulting peptides with minimal lateral diffusion. Silane chemistries are used to differentially treat the opposing surfaces of a glass filter paper to enable this unique capability. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PEPTIDES
*MASS spectrometry
*SILANE
*FILTER paper
*PROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 87
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 102451415
- Full Text :
- https://doi.org/10.1021/ac503980x