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Effect of hypoxia on calcium channels depends on extracellular calcium in CA1 hippocampal neurons
- Source :
-
Brain Research . Aug2003, Vol. 980 Issue 1, p128. 7p. - Publication Year :
- 2003
-
Abstract
- Our previous studies have shown that short lasting hypoxia induces an increase of Ca2+ influx into the cell through high voltage-activated Ca2+ channels in hippocampal neurons. This effect was abolished by removing of free Ca2+ from intracellular solution. The aim of this study was to compare hypoxic responses at different extracellular Ca2+ concentrations ([Ca2+]e) in hippocampal neurons to ascertain whether the hypoxic sensitivity is restricted to Ca2+ ions. Whole-cell patch-clamp recordings were made from acutely dissociated CA1 hippocampal neurons of rats. Polarographic method for measurements of O2 partial pressure was used. Here we found that at 2 mM [Ca2+]e the hypoxic effect was significant (up to ∼94%), whereas [Ca2+]e elevations to 5 and 15 mM resulted in gradual decreasing of the effect. We found, that total Ca2+ charge carried into the cell under the hypoxia was similar at all [Ca2+]e, whereas Ca2+ charge carried at normoxia was different for different [Ca2+]e, being larger at higher [Ca2+]e. These data indicated a saturation of the hypoxic effect due to limitation in the channel conductance. Therefore, we suggested that the hypoxic effect can be connected with increase of channel conductance, and the level of channel conductance at normoxia can determine the amplitude of hypoxic effect. [Copyright &y& Elsevier]
- Subjects :
- *HYPOXEMIA
*CALCIUM channels
*NEURONS
Subjects
Details
- Language :
- English
- ISSN :
- 00068993
- Volume :
- 980
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Brain Research
- Publication Type :
- Academic Journal
- Accession number :
- 10233515
- Full Text :
- https://doi.org/10.1016/S0006-8993(03)02951-2