The Expression of Hepatic Cytoskeletal Differential Protein Following Reduce-size Liver Transplantation in Rats.

Objective To analyze the changes of the expression of hepatic cytokeletal differential proteins by proteomics after reduce-size liver transplantation (RSLT) in rats. Methods The graft specimens were procured by Id, 3d and 7d following RSLT when the improved model of RSLT with Lewis-Wistar was finished. Then, the postoperative liver tissues, normal donor and recipient liver tissues harvested at the beginning were performed two-dimensional electrophoresis (2-DE) for differential protein stains. At the last, these differential proteins stains were analyzed and identified by MS-MS spectrometry and data bases analysis. Results In proteomics experiment, the selection criterion of differential protein was at least 10 times changes for protein stains, and there were 72 differential protein stains selected. The thirty-two of which were relatively clear functional protein. There were 3 differential protein taking part in pathway of cytokeletal process, expression of proteins in the total proteins ratio was 7 %. Conclusions The expression variation of cytokeletal protein following RSLT in rat has been gained by proteomics. It may play an important role and has significance in further studying the action and inherent mechanism of cytokeletal protein within hepatic pathophysiological process after RSLT. [ABSTRACT FROM AUTHOR]