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Modular microfluidics for point-of-care protein purifications.
- Source :
-
Lab on a Chip . 2015, Vol. 15 Issue 8, p1799-1811. 13p. - Publication Year :
- 2015
-
Abstract
- Biochemical separations are the heart of diagnostic assays and purification methods for biologics. On-chip miniaturization and modularization of separation procedures will enable the development of customized, portable devices for personalized health-care diagnostics and point-of-use production of treatments. In this report, we describe the design and fabrication of miniature ion exchange, size exclusion and affinity chromatography modules for on-chip clean-up of recombinantly-produced proteins. Our results demonstrate that these common separations techniques can be implemented in microfluidic modules with performance comparable to conventional approaches. We introduce embedded 3-D microfluidic interconnects for integrating micro-scale separation modules that can be arranged and reconfigured to suit a variety of fluidic operations or biochemical processes. We demonstrate the utility of the modular approach with a platform for the enrichment of enhanced green fluorescent protein (eGFP) from Escherichia coli lysate through integrated affinity and size-exclusion chromatography modules. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14730197
- Volume :
- 15
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Lab on a Chip
- Publication Type :
- Academic Journal
- Accession number :
- 101842219
- Full Text :
- https://doi.org/10.1039/c5lc00094g