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Pyridine-Biquinoline-Metal Complexes for Sensing Pyrophosphate and Hydrogen Sulfide in Aqueous Buffer and in Cells.
- Source :
-
Analytical Chemistry . 3/3/2015, Vol. 87 Issue 5, p2678-2684. 7p. - Publication Year :
- 2015
-
Abstract
- Herein, we report a new pyridine-biquinoline-derivative fluorophore L for effectively sensing pyrophosphate (PPi) and monohydrogen sulfide (HS-) in aqueous buffer and in living cells. L could selectively coordinate with metal ions (Mn+) in Groups IB and IIB to form L-Mn+ complexes with 1:1 stoichiometry, resulting in fluorescence quenching via photoinduced electron transfer (PET) mechanism. L-Zn2+ complex was applied to competitively coordinate with PPi to form a new "ate"-type complex, turning on the fluorescence by a 21-fold-increase. The limit of detection (LOD) of this assay for PPi detection in aqueous buffer is 0.85 μM. L-Cu2+ complex was applied for highly selective detection of HS- with an excellent sensitivity by 25-fold decomplexation-induced fluorescence increase. LOD of L-Cu2+ complex for HS- detection in aqueous buffer is 2.24 μM. With the in vitro data obtained, we successfully applied these two complexes for sequential imaging Zn2+ and PPi, Cu2+ and HS- in living cells, respectively. Since PPi and HS- occur in vascular calcification in positive correlation, our multifunctional probe L might help doctors to more precisely diagnose this disease in vivo in the future. For example, we could use radioactive tracer L-64Cu for qualitative and quantitative positron emission tomography/computed tomography (PET/CT) imaging of HS- in vivo. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PYRIDINE
*HYDROGEN sulfide
*NONCARBOXYLIC acids
*PYROPHOSPHATES
*SPECTRUM analysis
Subjects
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 87
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 101827562
- Full Text :
- https://doi.org/10.1021/ac504536q