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Detection of BRAF Mutations on Direct Smears of Thyroid Fine-Needle Aspirates Through Cell Transfer Technique.
- Source :
-
American Journal of Clinical Pathology . 2015, Vol. 143 Issue 4, p500-504. 5p. 1 Chart. - Publication Year :
- 2015
-
Abstract
- Objectives: To determine the utility of the cell transfer technique (CTT) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens. Methods: Polymerase chain reaction (PCR)-based BRAF molecular testing was performed on tissues obtained through CTT from both air-dried and ethanol-fixed direct smears of thyroid FNA specimens and then compared with the corresponding thyroidectomy formalin-fixed, paraffin-embedded (FFPE) tissues on 30 cases. Results: BRAF testing was successfully performed on 29 of 30 air-dried CTT, 27 of 30 ethanol-fixed CTT, and 27 of 30 FFPE tissues. The results exhibited 11, 13, and 13 BRAF mutations and 18, 14, and 14 wild types for the air-dried CTT, the ethanol-fixed CTT, and the FFPE tissues, respectively. The concordance rate was 96% between air-dried and ethanol-fixed CTT tissues, 88% between air-dried CTT and FFPE tissues, and 92% between ethanol-fixed CTT and FFPE tissues. Conclusions: PCR-based BRAF mutational testing can be reliably performed on the direct smears of the thyroid FNA specimens through the application of CTT. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BRAF genes
*GENETIC mutation
*BIOPSY
*CLINICAL pathology
*POLYMERASE chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 00029173
- Volume :
- 143
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- American Journal of Clinical Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 101822156
- Full Text :
- https://doi.org/10.1309/AJCP5BG0KUEOJCVS