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Bromodeoxyuridine labelling and fluorescence-activated cell sorting of polyamine-transforming bacterioplankton in coastal seawater.
- Source :
-
Environmental Microbiology . Mar2015, Vol. 17 Issue 3, p876-888. 13p. - Publication Year :
- 2015
-
Abstract
- Polyamines ( PAs) are a group of nitrogen-rich dissolved organic nitrogen ( DON) compounds that are ubiquitously distributed in marine environments. To identify bacteria that are involved in PA transformations, coastal bacterioplankton microcosms were amended with a single PA model compound, i.e. putrescine ( PUT) or spermidine ( SPD), or with no addition as controls ( CTRs). Bromodeoxyuridine ( BrdU) was added to all the microcosms to label newly synthesized DNAs. Fluorescence-activated cell sorting ( FACS) analysis indicated significant increases in numbers of total cells and cells with both high and low levels of BrdU incorporation in the PUT and SPD microcosms, but not in the CTRs. 16 S rDNA pyrotag sequencing of FACS-sorted cells indicated that PUT- and SPD-transforming bacteria were composed similarly of a diverse group of taxa affiliated with A ctinobacteria, B acteroidetes, F irmicutes and P roteobacteria (especially Roseobacter of its alpha lineage). Broad taxonomic distribution of PA-transforming bacteria was also indicated by the abundance and distribution of PA transporter gene homologues in a survey of sequenced marine bacterial genomes. Our results suggest that PAs may be common DON substrates for marine bacterioplankton, in line with the hypothesis that bacterially mediated PA transformation accounts for an important proportion of marine DON flux. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14622912
- Volume :
- 17
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Environmental Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 101588932
- Full Text :
- https://doi.org/10.1111/1462-2920.12550