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Jinlida reduces insulin resistance and ameliorates liver oxidative stress in high-fat fed rats.
- Source :
-
Journal of Ethnopharmacology . Mar2015, Vol. 162, p244-252. 9p. - Publication Year :
- 2015
-
Abstract
- Ethnopharmacological relevance Jinlida (JLD) is a compound preparation formulated on the basis of traditional Chinese medicine and is officially approved for the treatment of type 2 diabetes (T2DM) in China. We aimed to elucidate the mechanism of JLD treatment, in comparison to metformin treatment, on ameliorating insulin sensitivity in insulin resistant rats and to reveal its anti-oxidant properties. Materials and methods Rats were fed with standard or high-fat diet for 6 weeks. After 6 weeks, the high-fat fed rats were subdivided into five groups and orally fed with JLD or metformin for 8 weeks. Fasting blood glucose (FBG), fasting blood insulin, blood lipid and antioxidant enzymes were measured. Intraperitoneal glucose tolerance test (IPGTT) and hyperinsulinemic euglycemic clamp technique were carried out to measure insulin sensitivity. Gene expression of the major signaling pathway molecules that regulate glucose uptake, including insulin receptor (INSR), insulin receptor substrate-1 (IRS-1), phosphoinositide-3-kinase (PI3K), protein kinase beta (AKT), and glucose transporter type 2 (GLUT2), were assessed by quantitative RT-PCR. The totle and phosphorylation expression of IRS-1, AKT, JNK and p38MAPK were determined by Western blot. Results Treatment with JLD effectively ameliorated the high-fat induced hyperglycemia, hyperinsulinemia and hyperlipidemia. Similar to metformin, the high insulin resistance in high-fat fed rats was significantly decreased by JLD treatment. JLD displayed anti-oxidant effects, coupled with up-regulation of the insulin signaling pathway. The attenuation of hepatic oxidative stress by JLD treatment was associated with reduced phosphorylation protein levels of JNK and p38MAPK. Conclusions Treatment with JLD could moderate glucose and lipid metabolism as well as reduce hepatic oxidative stress, most likely through the JNK and p38MAPK pathways. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BLOOD sugar analysis
*LIVER physiology
*ALTERNATIVE medicine
*ANIMAL experimentation
*ANTIOXIDANTS
*BIOPHYSICS
*CELLULAR signal transduction
*CHOLESTEROL
*COMPARATIVE studies
*ENZYMES
*FAT content of food
*GLUCOSE tolerance tests
*HERBAL medicine
*HYPOGLYCEMIC agents
*INSULIN
*INSULIN resistance
*RESEARCH methodology
*CHINESE medicine
*ORAL drug administration
*PHOSPHORYLATION
*POLYMERASE chain reaction
*PROTEIN kinases
*RATS
*WESTERN immunoblotting
*STATISTICAL significance
*OXIDATIVE stress
*METFORMIN
*REVERSE transcriptase polymerase chain reaction
*DESCRIPTIVE statistics
*PHARMACODYNAMICS
*PREVENTION
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 162
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 101016886
- Full Text :
- https://doi.org/10.1016/j.jep.2014.12.040