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DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition.

Authors :
Xiao-Qin Jiang
Su-Miao Guo
Min Zhang
Ming Zhou
Bang-Ce Ye
Source :
Analyst. 2014, Vol. 139 Issue 22, p5682-5685. 4p.
Publication Year :
2014

Abstract

A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00032654
Volume :
139
Issue :
22
Database :
Academic Search Index
Journal :
Analyst
Publication Type :
Academic Journal
Accession number :
100818124
Full Text :
https://doi.org/10.1039/c4an01373e