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Nickel hydroxide/hydroxyapatite nanorods as affinity adsorbents for separation histidine-tagged proteins.
- Source :
-
Materials Research Bulletin . Apr2015, Vol. 64, p200-206. 7p. - Publication Year :
- 2015
-
Abstract
- Nickel hydroxide/hydroxyapatite (designated as Ni(OH) 2 /HAP) nanorods have been designed and synthesized by hydrothermal method. The size and morphology of Ni(OH) 2 /HAP sample depend strongly on the reaction condition, and introduction of small amount of Ni 2+ ions would be propitious to get the smaller size nanorods. By taking advantage of the high affinity of Ni 2+ ions toward histidine-tagged (His-tagged) proteins, the Ni(OH) 2 /HAP nanorods can be used to enrich and separate His-tagged proteins directly from a mixture of lysed cells, and their universality was evaluated by separating three kinds of His-tagged proteins. The Ni(OH) 2 /HAP nanorods present negligible nonspecific protein adsorption and high protein binding ability, and their specificity and affinity toward His-tagged proteins can be remained after 4 times recycling. The Ni(OH) 2 /HAP nanorods may provide many potential applications for the detection or separation of different biomolecules. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00255408
- Volume :
- 64
- Database :
- Academic Search Index
- Journal :
- Materials Research Bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 100760093
- Full Text :
- https://doi.org/10.1016/j.materresbull.2014.12.070