Back to Search
Start Over
Beneficial Effects of Fractions of Nardostachys jatamansi on Lipopolysaccharide-Induced Inflammatory Response.
- Source :
-
Evidence-based Complementary & Alternative Medicine (eCAM) . 2014, Vol. 2014, p1-11. 11p. 3 Diagrams, 6 Graphs. - Publication Year :
- 2014
-
Abstract
- It has been previously shown that Nardostachys jatamansi (NJ) exhibits anti-inflammatory properties against lipopolysaccharide (LPS) challenges. However, the potency of NJ constituents against LPS-induced inflammatory responses has not been examined. In this present study, we determined which NJ extract fractions exhibit inhibitory effects against LPS-induced inflammatory responses. Among the NJ fractions, NJ-1, NJ-3, NJ-4, and NJ-6 inhibited LPS-induced production of NO. The NJ-3, NJ-4, and NJ-6 fractions also inhibited the production of cytokines, such as IL-1β, IL-6, and TNF-α.However,NJ-1, NJ-3,NJ-4, andNJ-6 showed differential inhibitory mechanisms against LPS-induced inflammatory responses. NJ-1, NJ-3, and NJ-4 inhibited LPS-induced activation of c-jun NH2-terminal kinase (JNK) and p38 but did not affect activation of extracellular signal-regulated kinase (ERK) or NF-κB. On the other hand, NJ-6 inhibited activation of MAPKs and NF-κB. In addition, in vivo experiments revealed that administration of NJ-1, NJ-3, NJ-4, and NJ-6 reduced LPS-induced endotoxin shock, with NJ-6 especially showing a marked protective effect. Taken together, these results provide the evidence for the potential of selective NJ fractions against LPS-induced inflammation. Thus, it will be advantageous to further isolate and determine single effective compounds from these potent fractions. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENDOTOXEMIA prevention
*ENZYME metabolism
*ALTERNATIVE medicine
*ANALYSIS of variance
*ANIMAL experimentation
*ANTI-inflammatory agents
*BIOLOGICAL assay
*BIOLOGICAL models
*BIOPHYSICS
*CELLULAR signal transduction
*CYTOKINES
*ENZYME-linked immunosorbent assay
*HIGH performance liquid chromatography
*INTERLEUKINS
*MACROPHAGES
*RESEARCH methodology
*MEDICINAL plants
*MICE
*NITRIC oxide
*POLYMERASE chain reaction
*PROBABILITY theory
*PROTEIN kinases
*RESEARCH funding
*STAINS & staining (Microscopy)
*STATISTICS
*TUMOR necrosis factors
*WESTERN immunoblotting
*DNA-binding proteins
*PLANT extracts
*DATA analysis
*DATA analysis software
*DESCRIPTIVE statistics
*IN vitro studies
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Volume :
- 2014
- Database :
- Academic Search Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 100528092
- Full Text :
- https://doi.org/10.1155/2014/837835