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Acoustic actuated fluorescence activated sorting of microparticles.
- Source :
-
Lab on a Chip . 2014, Vol. 14 Issue 11, p1943-1950. 8p. - Publication Year :
- 2014
-
Abstract
- In this paper, we present a fluorescence activated sorter realized in a continuous flow microfluidic chip. Sorting is achieved by deflecting a focused particle stream with short acoustic bursts (2.5 ms), in a fluorescence activated configuration. The system utilizes two-dimensional acoustic pre-focusing, using a single actuation frequency, to position all particles in the same fluid velocity regime at flow rates up to 1.7 mL min−1. Particles were sorted based on their fluorescence intensities at throughputs up to 150 particles s−1. The highest purity reached was 80% when sorting at an average rate of 50 particles s−1. The average recovery of a sort was 93.2 ± 2.6%. The presented system enables fluorescence activated cell sorting in a continuous flow microfluidic format that allows aseptic integration of downstream microfluidic functionalities, opening for medical and clinical applications. [ABSTRACT FROM AUTHOR]
- Subjects :
- *FLUORESCENCE
*MICROFLUIDICS
*PROPERTIES of canal rays
*OPTICAL properties
*FLUIDICS
Subjects
Details
- Language :
- English
- ISSN :
- 14730197
- Volume :
- 14
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Lab on a Chip
- Publication Type :
- Academic Journal
- Accession number :
- 100410850
- Full Text :
- https://doi.org/10.1039/c3lc51408k