Analysis of biological composites inducing SD rat bone marrow mesenchymal stem differentiate into osteoblasts in vitro.

Objective To explore the ability of the biological composite materials. HA-glass coating/porous Zr02, inducing SD rat bone marrow mesenchymal stem cells to differentiate into osteoblasts in vitro, to find the ideal composite carrier of transplantated bone marrow mesenchymal stem cells and to investigate the security after transplantation, and to lay foundation for the fast recovery treatment of bone defect. Methods SD rat bone marrow mesenchymal stem cells were induced to differentiate by biological materials in vitro. Then cells were cultured for 14 days and 21 days. After dying with calcium-cobalt according to Gomori, Von Kossa staining, and immunohislochemical technology, the expression of alkaline phosphatase, calcium nodules, and type 1 collagen was detected. The changes of the expression of CD45, CD44, and CD90 in the cells before and after the induction were detected using flow cytometry. Results After 14-day induction in vitro, the cell morphology of SD rat bone marrow mesenchymal stem cells changed from long shuttle deformation cells to triangle or polygon cells, and there were clear boundaries between the cells. The expression of alkaline phosphatase was positive. After 21-day induction, dense and circular mineralized nodules appeared in cells, and the expression of type I collagen was positive. The boundaries between cells were blurred. According to the results of flow cytometry technology, the expression of CD44 was still positive, and the expression of CD45 was still negative. But the expression of CD90 transformed from positive to negative. The positive expression rate of cells decreased from 99.1% to 0- 29%. Conclusion Biological composite materials, HA-glass coating/porous ZiQ2 scaffolds, successfully induce SD rat bone marrow mesenchymal stem cells to osteoblasts. These materials can combine with bone formation through strong chemical bond, which can be used as bone defect tilling materials, and provide support for the formation of new bone, and play the conduction role in bone. [ABSTRACT FROM AUTHOR]