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The aim of this study was to investigate the insecticidal properties of essential oil derived from Eucalyptus cinerea leaves and its main component, 1,8-cineole, against two significant pests of stored dates, Ectomyelois ceratoniae and Ephestia kuehniella (Pyralidae). The impact of the treatment on the sensory characteristics of date fruits was assessed to verify the complete absence of off-odours and off-flavours. Gas chromatography – mass spectrometry analysis of E. cinerea essential oil revealed that the primary compound was 1,8-cineole (74.5%). Fumigant toxicity of the two pests, indicated that first instar larvae (L1) exhibited greater tolerance to E. cinerea EO and 1,8-cineole than L5, while adult forms were more susceptible than larvae. The E. cinerea EO had a noticeable pest contact activity when used at a concentration of 0.35 µL/cm2 caused 100 and 88.3% mortality to E. ceratoniae and E. kueheniella, respectively, after 1 hour of exposure. Hedonic evaluation showed that consumers' appreciation of fumigated dates was not significantly different to the no treated dates. [ABSTRACT FROM AUTHOR]

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Research on the biodegradation of polyethylene (PE), polystyrene (PS) and related polymers has become popular and the number of publications on this topic is rapidly increasing. However, there is no convincing evidence that the frequently claimed biodegradability of these so-called "plastics" really exists. Rather, a diffuse definition of the term "biodegradability" has led to the publication of reports showing either marginal weight losses of hydrocarbon polymers by the action of isolated bacterial strains or mechanical disintegration and polymer surface modification in case of hydrocarbon polymer-consuming insect larvae. Most of the data can be alternatively explained by the utilization of polymer impurities/additives, by the utilization of low molecular weight oligomers, and/or by physical fragmentation and subsequent loss of small fragments. Evidence for a (partial) biotic and/or abiotic oxidation of the amorphous polymer fraction and of surface-exposed hydrocarbon side chains is not sufficient to claim that PE is biodegradable. To the best of my knowledge, no report has been so far published in which substantial biodegradation and mineralization of PE or related (long chain length) hydrocarbon polymers to carbon dioxide has been convincingly demonstrated by the determination of the fate of carbon atoms in isotope-labeled polymers. It is disappointing that publications with a critical view on biodegradation of hydrocarbon polymers are not cited in most of these reports. The possibility should be considered that the rapidly expanding research field of hydrocarbon polymer biodegradation is chasing rainbows. • The term "Biodegradation" is not uniformly used in life sciences. • "Biodegradation" should be defined as conversion of an organic compound to H 2 O and CO 2. • Published evidence for biodegradation of hydrocarbon polymers and PVC is not convincing. • Experiments with isotope-labeled hydrocarbon polymers and PVC are necessary. [ABSTRACT FROM AUTHOR]

Trout-perch (Percopsis omiscomaycus) is an abundant, small-bodied, benthic fish species often found in deeper regions of large lake ecosystems. Historical evidence suggests that Trout-perch may participate in nearshore lake food webs by migrating from deep to shallow areas at night. However, little is known about Trout-perch ecology or their potential role in nearshore food webs. We used Trout-perch abundance estimates, diet analysis, and benthic invertebrate community surveys to (1) assess Trout-perch trophic ecology in Lake Champlain, USA/CAN, and (2) determine whether deepwater prey resources existed at sufficient densities to support Trout-perch populations or whether supplemental prey resources were required. Trout-perch showed variability in size and biomass across lake regions, but diet patterns were largely consistent across the lake, with > 50% of diets consisting of emergent insect larvae and oligochaetes. Results suggest that migrations into nearshore habitats may be required to sustain Lake Champlain's Trout-perch populations, and therefore, Trout-perch could function as conduits of allochthonous energy across distinct lake habitat boundaries. This study is one of only a few that have examined the trophic ecology of Trout-perch in deep lakes and fundamentally improves our understanding of energy flow and coupling between distinct lake food web compartments in large lakes. [ABSTRACT FROM AUTHOR]

ABSTRACT Silverleaf whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a destructive insect pest damaging to diverse crops by vectoring several plant pathogenic viruses, which consequently causes economic losses in crop production. As the resistance of whiteflies to chemical insecticides is increasing, this study aimed to investigate the potential of entomopathogenic fungi as an alternative. A total of 72 entomopathogenic fungal isolates, collected from soils using Tenebrio molitor larvae as an insect baiting method, were assessed for their virulence against 2nd nymphs of whitefly. Their virulence was assayed by dipping whitefly‐infested tomato leaves in fungal conidia suspensions at 1.0 × 107 conidia/mL. Among the tested isolates, two isolates of Beauveria bassiana JEF‐462 and JEF‐507 showed high virulence. In the assessment of virulence depending on conidia concentrations, the estimated LC50 values for JEF‐462 and JEF‐507 were similarly 8.7–14.0 × 107 conidia/mL. However, B. bassiana JEF‐507 showed higher conidial productivity and thermotolerance on most of tested 12 grain substrates than B. bassiana JEF‐462, and millet was the most suitable grain substrate. Additionally, siloxane as a surfactant was able to sufficiently exhibit the insecticidal activity of JEF‐507 against whitefly nymphs compared with other surfactants. In a pot‐based greenhouse trial, JEF‐507 showed higher control efficacy than chemical insecticides, dinotefuran and spinetoram. This work suggests that B. bassiana JEF‐507 could be competitively used as a biopesticide to control silverleaf whiteflies while overcoming current resistance issues. The JEF‐507 isolate has been registered in Korea, 2022 and successfully commercialised as the name of Chongchae‐Stop in this local market to control whitefly and thrips. [ABSTRACT FROM AUTHOR]

Dietary generalist herbivorous insects are widespread and often occur in a variety of environments. Across their geographic range, herbivorous insects may encounter variable plant traits as they feed on high‐quality or low‐quality plants. Herbivorous insect larvae experience both bottom‐up (host plant) and top‐down (parasitoid) factors that affect survival. Host plant quality may affect larval growth and survival in that larvae feeding on low‐quality plants often suffer reduced fitness. However, herbivores on different host plants are also subject to different levels of parasitism. High‐quality plants confer stronger larval performance (higher survival, more offspring), but larvae may also face higher parasitism. In some herbivore species, diet mediates larval immune response. The generalist insect herbivore fall webworm (FW), Hyphantria cunea Drury (Lepidoptera: Erebidae), is a moth native to North America, and its larvae have considerable variance in their performance when reared on different host plants. We investigated whether diet affects the immune response in FW larvae when they are reared on different host plant species known to vary in food quality. We measured immune response by melanization of a nylon filament. We found significant differences in immune response across host plants, indicating that diet mediates immune response in FW larvae. Our study helps elucidate the factors that cause variation in immune response in a generalist herbivore. [ABSTRACT FROM AUTHOR]

Recent rise in desert rose production, the expanded cultivation of this species may promote infestation by certain insects and mites. One such insect, the fungus gnats (Bradysia sp), also known as fungus flies, have been damaging numerous ornamental plant species. This study reported the incidence of fungus gnats infesting desert rose plants in the municipality of Jataí - GO, Brazil. Throughout 2022, we evaluated 48 desert rose plants for parasitic occurrences through direct observation. Sticky traps were used to record adult insects. Damage to plant organs has been documented and the presence of parasites at various stages of development with digital photographs. The incidence of fungal gnats is confirmed by observing adult insects and larvae in the plant substrate and roots. Of the 48 plants evaluated, 39 showed signs of infestation. Symptoms of a fungus gnat attack on desert rose include small necrotic lesions on new roots, progressive root rot, and a withered caudex resembling dehydration. The fungus gnats attack can cause the desert rose caudex to wilt, reducing its size and losing part of its shape, fact that can compromise the commercialization of this crop. [ABSTRACT FROM AUTHOR]

The amount of global plastic waste on land or in marine environments is a critical environmental issue. Plastic biodegradation by microorganisms, insect larvae, and enzymes has become one of the most popular solutions due to the ability of this strategy to generate environmentally benign byproducts, addressing ecological plastic waste concerns. This study revealed the biodegradation of ethylene vinyl acetate (EVA) by the bacterial strain identified as Klebsiella aerogenes EM011, isolated from effective microorganisms. The study found that K. aerogenes EM011 can survive in a carbon-free medium for 30 days using EVA films as the sole energy source, decomposing 0.65 ± 0.04% of 1 g of EVA film. The surface changes of the film were detected using scanning electron microscopy after treatment with K. aerogenes EM011. In addition, elemental modifications were detected in the imaged area of the plastic surfaces by energy-dispersive X-ray spectroscopy. Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy analyses were conducted to detect changes in the functional groups and chemical components, elucidating alterations on the surface of the EVA films. Through these physicochemical analyses, the formation of carbonyl groups (C=O), ester groups (C–O), and hydroxyl groups (–OH) confirmed the oxidation of EVA. Furthermore, the oxidation led to the decomposition of the EVA film, resulting in changes in its thermal stability and molecular weight distribution. These findings show that the K. aerogenes EM011 strain plays a role in accelerating the biodegradation of EVA. [ABSTRACT FROM AUTHOR]

As an organic waste reducer, Black soldier fly (BSF) larvae are an alternative in overcoming organic waste problems. Environmental conditions, larval density, amount of waste, time of waste application, and type of waste are reasons why the existing organic waste treatment with BSF larvae still needs to be improved. The main objective of this research is to develop the potential of black soldier fly larvae in reducing various types of organic waste (vegetable, fruit, restaurant waste, and mixed waste). To achieve the main objective of this study, the steps taken were analyzing the environmental conditions at the research site, the density of larvae in the reactor, the amount of organic waste, and the time of waste feeding on larval survival and the effectiveness of organic waste reduction. To implement the stage, sample preparation, selection of waste samples, making reactors, starting waste treatment, and measuring the results were carried out. It was found that BSF larvae at temperatures up to 34 °C, pH 8 with a larval density of 200 larvae, feeding 100--200 mg/larvae/day for 21 days were able to reduce organic waste by 84.5% with a waste reduction index value of 4.02%, a digested feed conversion efficiency value of 16.15% and a larval survival rate of 100%. Environmental conditions, larval density, amount of waste, time of waste feeding, and type of waste fed succeeded in reducing organic waste quickly compared to other organic waste treatments. [ABSTRACT FROM AUTHOR]

Simple Summary: In recent years, confined pheasant farms have been gaining popularity in both stocking and the production of meat and eggs. When assessing the nutritional value of meat products, the focus is on the nutrient content, including the mineral content and the amino acid profile. The production performance, e.g., body weight gains, feed conversion, and the proportion of meat in the carcasses, are also not insignificant. At the same time, taking into account the feeding behaviour of pheasants, especially during the rearing period under farm conditions, varied dietary components are used, including meals of animal origin and mineral-and-vitamin supplements. The authors hypothesised that the introduction of insect meal to the diet, instead of plant protein feeds, would have an effect on the production performance and the nutritional components of the muscles. The study results obtained show that the addition of insect meal contributed to an improvement in the meat yield and the levels of certain minerals without significantly affecting the amino acid profile. A 20% addition of meal from the larvae of insects Hermetia illucens proved to be most beneficial. The aim of the study was to determine the effects of replacing soybean meal with insect meal on the body weight and the chemical composition of selected muscle groups of common pheasant females and males, including the mineral composition and the amino acid profile of the thigh and breast muscles. The study was conducted on three feeding groups, namely one control and two experimental groups. In the control group, plant feed components were used, which are commonly used to feed pheasants in confined breeding facilities. In the experimental groups, 100 g (group II) and 200 g (group III) portions of insect meal were introduced instead of the plant-protein components. The experiment used a preparation of insect larvae (Hermetia illucens) containing approximately 52% crude protein. The pheasant diet supplementation applied contributed to an increase in the proportion of muscles in the carcasses, with the highest effectiveness obtained for a 20% addition of insect meal. Lower and significant differences were noted in the feed conversion by birds from the experimental groups, as compared to the control group. The chemical composition of the birds' muscles also changed. The experimental groups exhibited higher protein and fat contents and a lower water content. No significant changes in the amino acid profile or the mineral composition of the muscles were noted. The few exceptions concerned the methionine levels in both muscle groups and the isoleucine levels in the breast muscles. In most cases, the mineral composition did not vary significantly (p < 0.05). When supplementing the diet of breeding pheasants for improving meatiness, a 20% addition of insect meal is recommended, which affects the production effect of this trait while reducing feed consumption and maintaining the fatty acid profile. [ABSTRACT FROM AUTHOR]

Insects have evolved complex sensory systems that are important for feeding, defence and reproduction. Parasitoid wasps often spend much time and effort in searching for concealed hosts with the help of specialized sensilla. However, the early evolution of such behaviour and sensilla is poorly known. We describe two fossil female wasps, †Tichostephanus kachinensis sp. nov. and †Tichostephanus longus sp. nov., from mid‐Cretaceous Kachin amber. Phylogenetic analyses based on morphological data retrieved †Tichostephanus as deeply nested within Evanioidea and closely related to extant Gasteruptiidae and Evaniidae. Both of these Cretaceous wasps possess features, e.g. coronal tubercles and flexible ovipositor sheaths, that indicate that they might have laid eggs in wood where their larvae possibly parasitized insect larvae. They have a peculiar and unique 'bottle brush' of sensilla close to the apex of their ovipositor sheaths, which has not been observed in any extant parasitoid wasps. These sensilla comprise many regularly arranged plate‐shaped setae, attached in relatively large sockets and with rows of longitudinal ridges. Such specialized sensilla perhaps served to enhance the ability to detect hosts inside wood. [ABSTRACT FROM AUTHOR]

Ergot alkaloid synthesis (eas) gene clusters found in several fungi encode biosynthesis of agriculturally and pharmaceutically important ergot alkaloids. Although the biosynthetic genes of the ergot alkaloid pathway have been well characterized, regulation of those genes is unknown. We characterized a gene with sequence similarity to a putative transcription factor and that was found adjacent to the eas cluster of Metarhizium brunneum, a plant symbiont and insect pathogen. Function of the novel gene, easR, was explored by CRISPR-Cas9-derived gene knockouts. To maximize potential for ergot alkaloid accumulation, strains of M. brunneum were injected into larvae of the insect Galleria mellonella. Larvae infected with the wild type contained abundant ergot alkaloids, but those infected with easR knockouts lacked detectable ergot alkaloids. The easR knockout strains had significantly reduced or no detectable mRNA from eas cluster genes in RNAseq and qualitative RT-PCR analyses, whereas the wild-type strain contained abundant mRNA from all eas genes. These data demonstrate that the product of easR is required for ergot alkaloid accumulation and provide evidence that it has a role in the expression of ergot alkaloid biosynthesis genes. Larvae infected with an easR knockout survived significantly longer than those infected with the wild type (P < 0.0001), indicating a role for EasR, and indirectly confirming a role for ergot alkaloids, in the virulence of M. brunneum to insects. Homologs of easR were found associated with eas clusters of at least 15 other ergot alkaloid-producing fungi, indicating that EasR homologs may contribute to regulation of ergot alkaloid synthesis in additional fungi. IMPORTANCE Ergot alkaloids produced by several species of fungi are important as contaminants of food and feed in agriculture and also as the foundation of numerous pharmaceuticals prescribed for dementia, migraines, hyperprolactinemia, and several other disorders. Information on control of the ergot alkaloid pathway may contribute to strategies to limit their production in agricultural settings or increase their yield for pharmaceutical production. Our results demonstrate that a previously uncharacterized gene clustered with the ergot alkaloid synthesis genes is required for the sufficient transcription of the ergot alkaloid biosynthesis genes. This observation suggests the gene encodes a factor regulating transcription of those biosynthetic genes. [ABSTRACT FROM AUTHOR]

• Aquaculture sludge represents a promising future feed material for insects. • BSF larvae can grow on a substrate solely composed of aquaculture sludge. • BSF larvae reduced aquaculture waste and accumulated valuable nutrients. • BSF larvae accumulated undesirable substances including Cd, Hg and dioxins. Aquaculture sludge (uneaten feed and faeces) is nutrient rich and has potential as feed for insects. The aim of this study was to investigate the transfer of chemical and biological contaminants, as well as nutrients, from aquaculture sludge to black soldier fly larvae. The larvae were reared on a sludge mixture made of different sludges collected from Norwegian freshwater salmonid facilities. The sludge was spiked with four common salmon pathogens: Infectious Pancreatic Necrosis Virus, Infectious Salmon Anemia virus, Yersinia ruckeri or Mycobacterium salmoniphilum. During the 15 days of growth on sludge, the black soldier fly larvae accumulated valuable nutrients including protein, fat, eicosapentaenoic acid, iron, manganese, zinc and selenium. The larvae also accumulated undesirable substances including cadmium, mercury, dioxins and polychlorinated biphenyls. The concentrations of dioxins exceeded the EU maximum level set for animal feed. None of the salmon pathogens that were spiked to the sludge were detected in the black soldier fly larvae. This study reports low risk of transfer of salmon pathogens from sludge to insect larvae, and showed that the transfer of heavy metals, minerals and metalloids are in accordance with earlier studies. The large variations in levels of heavy metals between batches of sludge can cause levels in BSF exceeding the EU maximum levels, and thus indicate a need for monitoring of the proposed value chain. The transfer of dioxins from sludge to insects, reported for the first time in this paper, would be of special interest for future research, with special focus on risk mitigation. [ABSTRACT FROM AUTHOR]

Background: The fleshfly Sarcophaga dux (Diptera;Sarcophagidae) is found in Indian Sub continent is of limited forensic significance as its poorly known larval development relationship with temperature and relative humidity. Objective: The objective of this study was to determine the effect of the temperature and relative humidity in the larval development of this fly in two seasons, Summer and Rain, so that the results of this study may enabling the forensic use of this fly in the estimation of Post Mortem Interval(PMI). Material and Method: The present study was carried out in the outer premises of the Regional Forensic Science Laboratory Building, Jagdalpur, Bastardist,Chhattishgarh, India. The fresh meat of male goat was used for rearing of larvae. The effect of temperature and relative humidity on the larval developmental time of this fly in two seasons, Rainy and Summer were studied in normal condition. Statistical analysis of variance was performed and standard significance level of P<0.05 was taken as standard. Results: The results of this study shown that in Rainy season it was completed in 264 h (11 days) when the average temperature ranged from 21.8°C to 24.5°C and the average humidity ranged from 79.5% to 95.5% where as in Summer it was completed in 528 hrs (22 days), when the temperature ranged from 26.5°C to 35.1°C and the humidity ranged from 34% to 67.5%. The P value was found to be P<<.05 in Temperature variation; andP<<0.05 in Humidity variation in different stages(1st instar larvae, 2nd instar larvae, 3rd instar larvae, Prepupae, Pupae and Adult). Conclusion: In the present study we have found that fluctuating temperature and relative humidity of outer condition in two seasons, Rainy and Summer effect the life cycle duration of the fly from the larvi position up to adult eclosion and statistical analysis has proved it to significant rejecting null hypothesis. This data can be further used in the estimation of Post Mortem Interval (PMI) in Homicide and Suspicious cases of both human and wild life poaching death cases. [ABSTRACT FROM AUTHOR]

Seasonal feeding activity and ontogenetic dietary shifts of Cyprinus carpio (Linnaeus, 1758) were examined using 209 fish samples from Lake Arekit, Ethiopia. Among the 209 specimens, 174 (83.3%) displayed a range of dietary items in their stomachs. The species' feeding patterns were determined through numerical indexes (frequency of occurrence and volumetric contribution) and composite indexes (index of preponderance [IOP] and Geometric Index of Importance [GIIi]). Detritus emerged as the primary food source for C. carpio, followed by macrophytes, insects and zooplankton. A lesser amount of phytoplankton, nematodes and ostracods were also consumed. On the basis of the findings, C. carpio can be classified as a detri‐omnivore, as it consumes benthic species like nematodes, ostracods and insect larvae. Seasonal fluctuations in food item consumption were noted (p < 0.05), with higher intake during the rainy season and reduced consumption in the dry season. There was a distinct ontogenetic shift in the relative importance of food items and showed significant variations in food preferences among size groups (p < 0.05). The contributions of animal‐origin food categories (ostracods, zooplankton and insects) were important in the diet of the smaller size class, whereas food of plant origin (macrophytes) and detritus were more important in the diet of adults. Understanding the seasonal feeding patterns and ontogenetic dietary shifts of C. carpio in Lake Arekit provides crucial insights into their ecological role within the lake's ecosystem and highlights their adaptability to eutrophic conditions. Common carp in Lake Arekit are opportunistic feeders, consuming a variety of detritus, plants, insects, and small animals. Their diet adapts based on size and season, with smaller fish preferring animal prey and larger fish consuming more plants and detritus. This study provides valuable insight into carp diet in this specific lake, but further research is needed to understand lifelong feeding patterns and environmental influences. [ABSTRACT FROM AUTHOR]

Myiasis is a parasitic infestation of fly larvae caused by dipterous larvae that live in the host's dead or living tissues. This study reported the first case of urogenital myiasis in infants from Iran caused by Lucilia sericata. The 10-month-old boy was taken to the hospital by his mother due to restlessness and abdominal pain. During the examination, motile and live larvae were observed in the penis area. The patient had no history of infectious disease or immune system deficiency, and his blood tests were normal. The entomological analysis found that the source of this myiasis was Lucilia sericata fly larvae. The report of such cases highlighted the importance of physical examination and the awareness of physicians in dealing with myiasis, as well as the necessity of educating mothers on the hygiene of the living environment and child care in rural areas. [ABSTRACT FROM AUTHOR]

Simple Summary: The bacterium Yersinia ruckeri causes enteric redmouth disease, which affects salmonid fish, including farmed fish such as Atlantic salmon and rainbow trout, leading to significant commercial losses. In this study, we investigated a plasmid (an independently replicating DNA molecule) called pYR4 that is suspected of aiding Y. ruckeri in causing disease by producing adhesive structures known as type 4 pili. We identified a toxin–antitoxin locus on the plasmid, higBA, which we hypothesized might prevent loss of the plasmid from the bacterial cell through "addiction" to the antitoxin HigA. HigA is quickly degraded if the plasmid is lost, whereas the toxin HigB is stable and leads to cell death in the absence of HigA. We were able to demonstrate that HigBA is a functional toxin–antitoxin system and that stabilizes pYR4. We further showed that pYR4 can transfer between bacteria by a process known as conjugation. However, loss of the pYR4 plasmid did not have an effect in a simple insect larva infection model. Further work will have to be undertaken to evaluate the role of pYR4 in enteric redmouth disease. These results will help in understanding and ultimately managing a serious disease of commercially important fish. The bacterium Yersinia ruckeri causes enteric redmouth disease in salmonids and hence has substantial economic implications for the farmed fish industry. The Norwegian Y. ruckeri outbreak isolate NVH_3758 carries a relatively uncharacterized plasmid, pYR4, which encodes both type 4 pili and a type 4 secretion system. In this study, we demonstrate that pYR4 does not impose a growth burden on the Y. ruckeri host bacterium, nor does the plasmid contribute to twitching motility (an indicator of type 4 pilus function) or virulence in a Galleria mellonella larval model of infection. However, we show that pYR4 is conjugative. We also reveal, through mutagenesis, that pYR4 encodes a functional post-segregational killing system, HigBA, that is responsible for plasmid maintenance within Y. ruckeri. This is the first toxin–antitoxin system to be characterized for this organism. Whilst further work is needed to elucidate the virulence role of pYR4 and whether it contributes to bacterial disease under non-laboratory conditions, our results suggest that the plasmid possesses substantial stability and transfer mechanisms that imply importance within the organism. These results add to our understanding of the mobile genetic elements and evolutionary trajectory of Y. ruckeri as an important commercial pathogen, with consequences for human food production. [ABSTRACT FROM AUTHOR]

Simple Summary: Defatting can be performed mechanically by cutting the frozen insect larvae and then pressing them to enable the leakage of intracellular fat, or chemically using petroleum ether extraction of the insect meal. This study aimed to investigate the effects of different defatting methods of black soldier fly (Hermetia illucens) larvae meal (BSFM) on the metabolic energy and nutrient digestibility in laying hens. The results show that both defatting methods of BSFM had no adverse effects on the metabolic energy and nutrient digestibility in young laying hens, but pressed black soldier fly meal (BSFMp) demonstrated better effects on the digestibility of metabolic energy and nutrients in the feed for young laying hens. This study aimed to investigate the effects of different defatting methods of black soldier fly (Hermetia illucens) larvae meal (BSFM) on the metabolic energy and nutrient digestibility in laying hens. Sixty young laying hens (Hy-Line W-36) aged 63 days were randomly divided into two groups (G1 and G2), each with five replicates of six hens housed in individual cages. Group G1 was fed 25% pressed black soldier fly meal (BSFMp) and 75% basal diet, and Group G2 was fed 25% extracted black soldier fly meal (BSFMe) and a 75% basal diet. Both diets included 5 g/kg chromium oxide as an external marker. A 7-day preliminary trial was followed by a 4-day experimental period. The results indicate that pressing and extracting significantly affected the digestibility of crude fat and total energy in BSFM, with BSFMp showing significantly higher crude fat digestibility than BSFMe. Similarly, total energy digestibility was also significantly higher in BSFMp. However, there were no significant differences in dry matter, organic matter, and crude protein digestibility between the two processing methods. The apparent metabolic energy values of BSFMp and BSFMe were 16.34 and 12.41 MJ/kg, respectively, showing a significant difference. The nitrogen-corrected metabolic energy values were 15.89 MJ/kg in BSFMp and 11.93 MJ/kg in BSFMe, indicating a highly significant difference. The digestibility of arginine and leucine in BSFMp was significantly higher than in BSFMe, while differences in lysine, cystine, threonine, tryptophan, and isoleucine were not significant. In conclusion, both defatting methods of BSFM had no adverse effects on the metabolic energy and nutrient digestibility in young laying hens, but BSFMp demonstrated better effects on the digestibility of metabolic energy and nutrients in the feed for young laying hens. [ABSTRACT FROM AUTHOR]

A new approach to supplying foods and feeds that are high in protein has emerged in the form of insect farming. Insect‐farmed food may also serve as part of a sustainable diet for humans. The mealworm Tenebrio molitor L. (Coleoptera: Tenebrionidae) is the most widely consumed insect worldwide. In this study, 10 larval diets were tested in the laboratory to determine their suitability for mass‐feeding mealworms at 28 °C and 60–65% r.h., in a completely randomized design. Various larval diets had a significant impact on the biological features of T. molitor. The larvae, pupae, and adults were greater on diets containing wheat bran alone and wheat bran in combination with maize and porridge. Larval mortality was significantly higher (60.7%) on a diet with porridge alone, compared to a control diet, followed by diets with porridge in combination with barley (55.6%) or maize (50.6%). Sex ratio was also affected by the tested larval diets. Female hatch (65%) was higher than male hatch (35%) on diet containing wheat bran alone as compared to all other diets. The findings contribute to optimizing mealworm rearing practices for sustainable food production and promote the potential use of mealworms as a viable protein source – especially livestock diets based on mealworm reared on wheat bran show promise. [ABSTRACT FROM AUTHOR]

Geographic isolation is a factor that is known to affect species composition and diversity in marine systems. In this study, we tested the effects of geographic isolation on invertebrate fauna living among the belt-forming foundation species Fucus vesiculosus in the northern Baltic Sea. Sampling was carried out on reefs with varying levels of isolation (0.5, 1 and 2 km) in July 2021 in the Åland islands (between 59.96°N 20.24°E and 59.75°N 20.84°E). The total number of taxa, abundance, Shannon-Wiener index, Pielou's evenness and biomass were calculated and the effects of isolation were tested using generalised linear models. Multivariate methods were used to analyse differences in the invertebrate community composition. Although the number of taxa and Shannon´s diversity index did not vary along the isolation gradient, the total abundance of fauna decreased with increasing isolation. In addition, some taxa-specific patterns were observed, e.g. the abundance of insect larvae decreased with isolation while isopods were more numerous in the most isolated locations. As diversity and the number of taxa were similar regardless of isolation, shallow Fucus vesiculosus belts likely act as important biodiversity refuges and potential stepping-stones for invertebrates in remote outer archipelago areas. In a changing climate, these kinds of refuges aiding species dispersal are likely to be even more important, and should be considered in conservation planning. [ABSTRACT FROM AUTHOR]

Sea level rise threatens ecosystem function in coastal lakes by increasing salinity and altering community composition. As mobile generalists, fish have considerable influence on the stability of a system by impacting trophic network structure. Understanding their diet across abiotic gradients, including salinity, can show key characteristics of their ecological role, and predator diet shifts may serve as warning signs of food web restructuring. Stomach contents analysis was used to look at the diet of Retropinna retropinna (common smelt) across shallow coastal lakes on Rēkohu (Chatham Island). Smelt diets from 10 lakes (seven freshwater; three salt-influenced) were analysed. Comparisons of salt-influenced and freshwater lakes showed shifts in composition. Smelt in freshwater lakes predominantly consumed herbivorous, omnivorous, or predatory insect larvae while diet in salt-influenced lakes was dominanted by crustacean shredders. Population diet varied somewhat less among salt-influenced lakes than among freshwater lakes, suggesting homogenisation of smelt diet with salinity. Differences in composition and heterogeneity did not translate to consistent differences in total niche width or individual specialisation between freshwater and salt-influenced lakes. Further development of knowledge on biological structure across salinity gradients is essential to understand ecosystem function under future climate scenarios. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Plant Protection & Pathology is the property of Egyptian National Agricultural Library (ENAL) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Osmylus fulvicephalus (Scopoli, 1763), the type species of the Osmylidae, has garnered significant research interest in taxonomy, particularly regarding its larval stages. To date, most studies have focused on the third instar larvae, but ignore the first instar larvae with the rough and incomplete morphological descriptions. This lack of studies has led to an unclear understanding of the morphological differences between larval stages, making it challenging to identify larvae, distinguish different osmylid species or even reconstruct phylogeny. New information: In this study, the first instar larva of O. fulvicephalus was re-described in detail and illustrated, based on the naming system of sclerites. Based on this, it could be effectively distinguished from other species by the following characteristics: i) number of segments in the labial palpi; ii) the setae beard on the apex of antenna; iii) the number of transversal rows of the anal hooks. In addition, we compared the sclerites and other morphological characteristics with the third instar larvae of Osmylus fulvicephalus to better distinguish larvae at different developmental stages. [ABSTRACT FROM AUTHOR]

Background: The black soldier fly (BSF), Hermetia illucens, is known for nutrient-recycling through the bioconversion of organic waste into protein-rich insect larvae that can be processed into an animal feed ingredient. However, information on species distribution and its genetic structure in Iran is scarce. Methods and results: We directed a survey on the Caspian Sea coast, with a reconstructing demographic relationships study using two parts of mitochondrial cytochrome C oxidase 1 (COI) gene (barcode and 3' end regions) and nuclear internal transcribed spacer 2 (ITS2) to identify BSF' genetic diversity in retrospect to the global diversity and the potential origin of the Iranian BSF population. Larvae and adults were recovered from highly decomposed poultry manure, in May 2020. Sequence analysis of both regions of COI gene (about 1500 bp) revealed a single haplotype, identical to that of haplotype C, a worldwide commercial strain originated from Nearctic, Palearctic, or African biogeographic regions. However, the ITS2 locus was confirmed to be invariable across samples from diverse biogeographic regions. Conclusion: The results proved the presence of BSF in north of Iran. However, it is not possible to determine with certainty when and where this species first established in Iran, and they have likely been released to nature due to the existence of companies importing and breeding such flies. Due to heavy international trading, the introduction and settlement of this fly in the southern coasts of the country is highly suggested. [ABSTRACT FROM AUTHOR]

This study evaluates the seasonal and inter-seasonal temperature regime of small tundra ponds ubiquitous to an extensive, low-gradient wetland in the Canadian High Arctic. Pond temperatures can modify evaporation and ground thaw rates, impact losses of greenhouse gases, and control the timing and emergence of insects and larvae critical for migratory-bird feeding habits. We focus our study on thaw ponds with a range of hydrologic linkages and sizes across Nanuit Itillinga, formerly known as Polar Bear Pass (PBP), Bathurst Island, and compare their thermal signals to other Arctic ponds. Pond temperatures and water levels were evaluated using temperature and water level loggers and verified by regular manual measurements. Other environmental data collected included microclimate, frost table depths, and water conductivity. Our results show that there is much variability in pond thermal regimes over seasons, years, and space. Cumulative relative pond temperatures were similar across years, with ponds normally reaching 10–15 °C for short to longer periods, except in 2013, which experienced a cold summer season during which pond temperatures never exceeded 5 °C. Pond frost tables and water conductivities respond to variable substrate conditions and pond thermal patterns. This study contributes to the ongoing discussion on climate warming and its impact on Arctic landscapes. [ABSTRACT FROM AUTHOR]

Infections by drug-resistant microorganisms are a threat to global health and antimicrobial peptides are considered to be a new hope for their treatment. Temporin-WY2 was identified from the cutaneous secretion of the Ranidae frog, Amolops wuyiensis. It presented with a potent anti-Gram-positive bacterial efficacy, but its activity against Gram-negative bacteria and cancer cell lines was unremarkable. Also, it produced a relatively high lytic effect on horse erythrocytes. For further improvement of its functions, a perfect amphipathic analogue, QUB-1426, and two lysine-clustered analogues, 6K-WY2 and 6K-1426, were synthesised and investigated. The modified peptides were found to be between 8- and 64-fold more potent against Gram-negative bacteria than the original peptide. Additionally, the 6K analogues showed a rapid killing rate. Also, their antiproliferation activities were more than 100-fold more potent than the parent peptide. All of the peptides that were examined demonstrated considerable biofilm inhibition activity. Moreover, QUB-1426, 6K-WY2 and 6K-1426, demonstrated in vivo antimicrobial activity against MRSA and E. coli in an insect larvae model. Despite observing a slight increase in the hemolytic activity and cytotoxicity of the modified peptides, they still demonstrated a improved therapeutic index. Overall, QUB-1426, 6K-WY2 and 6K-1426, with dual antimicrobial and anticancer functions, are proposed as putative drug candidates for the future. [ABSTRACT FROM AUTHOR]

This study aimed to examine the bacterial, methanogenic archaeal, and eukaryotic community structure in both the midgut and hindgut of Pachnoda marginata larvae using an amplicon sequencing approach. The goal was to investigate how various diets and the soil affect the composition of these three‐domain microbial communities within the gut of insect larvae. The results indicated a notable variation in the microbial community composition among the gut compartments. The majority of the bacterial community in the hindgut was composed of Ruminococcaceae and Christensenellaceae. Nocardiaceae, Microbacteriaceae, and Lachnospiraceae were detected in midgut samples from larvae feeding on the leaf diet, whereas Sphingomonadaceae, Rhodobacteraceae, and Promicromonasporaceae dominated the bacterial community of midgut of larvae feeding on the straw diet. The diet was a significant factor that influenced the methanogenic archaeal and eukaryotic community patterns. The methanogenic communities in the two gut compartments significantly differed from each other, with the midgut communities being more similar to those in the soil. A higher diversity of methanogens was observed in the midgut samples of both diets compared to the hindgut. Overall, the microbiota of the hindgut was more host‐specific, while the assembly of the midgut was more influenced by the environmental microorganisms. [ABSTRACT FROM AUTHOR]

Scavenging is a key process for the cycling of nutrients in ecosystems, yet it is still neglected in the ecological literature. Apart from the importance of specific groups of animals in scavenging, there have been few ecological studies that compare them. Furthermore, the ecological studies on scavenging have mainly focused on vertebrates despite the crucial importance of invertebrates in this process. Here, we performed a large‐scale ant suppression and vertebrate exclusion experiment to quantify the relative contribution of ants, non‐ant invertebrates and vertebrates in scavenging nitrogen‐rich (insect carcasses) and carbon‐rich (seeds) baits in two contrasting mountainous habitats in Brazil (grasslands and forests). Overall, bait removal was 23.2% higher in forests than in grasslands. Ants were the primary scavengers in grasslands, responsible for more than 57% of dead insect larvae and seed removal, while, in forests, non‐ant invertebrates dominated, removing nearly 65% of all baits. Vertebrates had a minor role in scavenging dead insect larvae and seeds in both habitats, with <4% of removals. Furthermore, our results show that animal‐based baits were more consumed in forests than seeds, and both resources were equally consumed in grasslands. Therefore, we demonstrate the superiority of invertebrates in this process, with a particular emphasis on the irreplaceable role of ants, especially in this grassland ecosystem. As such, we further advance our knowledge of a key ecosystem process, showing the relative importance of three major groups in scavenging and the differences in ecosystems functioning between two contrasting tropical habitats. [ABSTRACT FROM AUTHOR]

Simple Summary: Mealworms (Tenebrio molitor) have a great ability to bioconvert vegetable by-products used as feed into body mass. Rice straw is a vegetable by-product that is difficult to dispose of and causes environmental problems. The use of physicochemical or enzymatic treatments to improve the digestibility of these by-products can increase the performance of mealworms in terms of yield. In this study, mealworm larvae were reared on diets formulated with rice straw and rice straw subjected to a combined treatment of laccase enzyme, ultrasound, and ascorbic acid to evaluate the impact on the growth performance and digestibility of T. molitor. The combined sonication, laccase enzyme, and ascorbic acid treatment of rice by-products facilitated their use by T. molitor larvae, increasing the weight gain, feed conversion ratio, and conversion efficiency of ingested feed. The use of treatments capable of improving the usefulness of vegetable by-products opens the possibility of developing new methodologies for their use for insect feed. The development of reuse processes for plant by-products for both animal and human food offers numerous possibilities for quality-of-life improvements that align with a circular economy model. For this reason, we divided this study into two experiments. First, we designed a combined treatment consisting of laccase, ultrasound, and ascorbic acid to hydrolyze rice straw plant fibers and used the resulting feed as the basis for T. molitor diets. Second, we formulated diets with different inclusion levels (0%, 25%, 50%, 75%, and 100%) of rice straw and treated rice straw to assess their impact on larvae growth and diet digestibility. For each treatment, six replicates were employed: four for the growth–performance–digestibility trial and two for complementary uric acid determination tests. The combined laccase enzyme, ultrasound, and ascorbic acid treatment hydrolyzed 13.2% of the vegetable fibers. The diets containing treated rice straw resulted in higher larvae weight and a better feed conversion ratio; however, reaching 100% by-product inclusion values led to similar results between both diets. In conclusion, these treatments improve the potential of low-nutritional-value vegetable by-products as part of a T. molitor diet, opening the possibility of new methodologies for the use of recalcitrant vegetable by-products for insect rearing. [ABSTRACT FROM AUTHOR]

Many studies have examined the gene expression of baculoviruses during host infection by infecting cultured insect host cells; however, only a few have attempted to characterize the interaction between baculoviruses and insect larvae, which more accurately models the virus‐insect relationship. The greatest challenge in assessing gene expression profiles in vivo in the larval gut is the lack of infection synchronization compared to cultured cells. Working with bertha armyworm, Mamestra configurata, larvae infected with the baculovirus Mamestra configurata nucleopolyhedrovirus‐A (MacoNPV‐A), viral gene expression was measured using droplet‐digital PCR, showing that the rate of infection in individual insects varies extremely widely. Subsequent RNA‐Seq of individual guts revealed that gene expression patterns were consistent in individuals with similar levels of viral gene expression. Therefore, the best approach for analysing baculovirus gene expression in vivo is to use the expression of an early viral gene as a means to select individuals that are closely matched in infection progress to produce the most synchronized infection cohorts for measuring gene expression profiles. In this way, a profile of gene expression was identified very early in infection that would have been masked by the activity of a few very robustly infected individuals present in sample pools made up of multiple individuals. [ABSTRACT FROM AUTHOR]

Continuous chemical dengue control can cause vector resistance and environmental pollution. Developing natural larvicides (bio-larvicides) from plant toxins like white chicory, which can poison Aedes aegypti larvae, is necessary for a sustainable alternative. This study aimed to analyze differences in active ingredients in white chicory leaves (Brassica pekinensis L) as bio-larvicide against Aedes aegypti larvae. This study was a pure experiment using a post-testonly control design using 1,225 Aedes aegypti instar III larvae with extracts of alkaloid active ingredients, flavonoids, and concentrations of 0%, 24%, 34%, and 40% with 3 times replication. Data were analyzed using a probit test, one-way ANOVA, and Post Hoc LSD. The results showed the potential of active ingredients in white chicory leaves against the death of Aedes aegypti larvae (p-value = 0.000). The average percentage of larval mortality concentration was 24%, 34%, 40%, in alkaloids was 41%, 60%, 66%, and in flavonoids was 45%, 64%, 68%. The active ingredient of white chicory leaves can kill Aedes aegypti larvae with LC50 in 29% alkaloids and 27% flavonoids. [ABSTRACT FROM AUTHOR]

This article discusses the discovery of a lacewing larva in Eocene amber that exhibits characteristics typically found in Cretaceous larvae. The presence of these characteristics suggests that certain morphologies thought to be extinct after the Cretaceous period may have actually survived into the Eocene. The study also highlights the differences between the Eocene and Baltic amber faunas and emphasizes that the Eocene entomofauna contained both ancient and modern morphologies. The research is supported by various institutions and references other studies on fossil insects found in amber. This document is a list of references for various scientific articles related to the study of insects preserved in amber, providing valuable information for researchers in the field of paleoentomology. [Extracted from the article]

Simple Summary: Soybean meal is the main constituent of poultry feed, and the global supply of soybeans has decreased because of an imbalance between its demand and supply, primarily caused by the growing world population and reduced cultivation area. Therefore, a cheap and environmentally sustainable alternative protein source is urgently needed to maintain the sustainability of poultry production. In this study, we demonstrated that substituting 12% of soybean meal with either fall armyworm larvae or black soldier fly larvae enhances the growth, blood haematology, gut morphometry, and meat quality of broiler chickens. Consequently, soybean meal can be substituted with protein-rich insects in the poultry feed. The primary challenge facing the global animal industry is the scarcity of protein feed resources. Various insects are gaining prominence as innovative feed sources due to their economic, environmentally friendly, and nutritious attributes. The purpose of the present study was to determine the effects of a partial replacement of soybean meal with fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) and black soldier fly Hermetia illucens (Diptera: Stratiomyidae) on the growth performances, blood parameters, gut histology, and meat quality of broilers. A total of 350 1-day-old (40 ± 0.15 g) male chicks (Ross 308) were randomly assigned to seven experimental meals. Each treatment was repeated five times with 50 birds per dietary treatment. The seven dietary treatments included 4, 8, and 12% replacements of SBM with larvae of S. frugiperda and H. illucens. SBM was the basal diet considered the control. The data showed that broilers fed 12% S. frugiperda or H. illucens exhibited a significantly higher (p < 0.05) live weight, average daily weight gain, and improved the feed conversion ratio. Meals with 12% S. frugiperda or H. illucens significantly enhanced (p < 0.05) haematological and gut histological parameters, including villus height, crypt depth, villus width, and villus height/crypt depth ratios. The meat of broilers fed the 12% S. frugiperda diet showed significantly higher (p < 0.05) lightness and yellowness. Replacing soybean meal up to 12% with either S. frugiperda or H. illucens larvae improves the growth performance, blood haematology, gut morphometry, and meat quality traits of broilers. [ABSTRACT FROM AUTHOR]

Background & Objectives: Mosquito-borne diseases significantly impact global health, particularly in tropical regions. While synthetic insecticides are currently employed to control mosquito vectors, their detrimental effects on ecosystems and persistence necessitate alternative control methods. Botanicals, owing to their diverse phytocompounds, offer potential for controlling and preventing vector-borne diseases by targeting insect eggs and larvae. This study aimed to evaluate the toxicity of Melissa officinalis and Rosmarinus officinalis extracts (methanolic and aqueous) against Aedes aegypti larvae, a vector of arboviruses. Materials & Methods: Total protein content in control and plant extract-exposed larval homogenates was estimated using bovine serum albumin as a standard. Acetylcholinesterase (AChE) and carboxylesterase assays were performed to determine larvicidal effects. Larval mortality was assessed after 24 hours of exposure. Results: Our findings revealed that the methanolic leaf extract of M. officinalis exhibited superior larvicidal activity (100% at 1000 ppm) compared to the methanolic R. officinalis extract (84±2.45% at 1000 ppm). In contrast, the aqueous extracts of both plant species inferred no larvicidal activity. The LC50 and LC90 values for M. officinalis methanolic extract were 378.7 ppm and 795.8 ppm, respectively, whereas those for R. officinalis were 648.9 ppm and 1152.9 ppm, respectively. Furthermore, biochemical assays measuring total protein, acetylcholinesterase, a-carboxylesterase, and ß-carboxylesterase activities were conducted for M. officinalis, corroborating and substantiating its larvicidal properties. Conclusion: This study demonstrates that the methanolic leaf extract of M. officinalis possesses significant larvicidal efficacy against A. aegypti. These findings suggest that this plant or its phytocompounds could serve as a bioinsecticide, offering a potential alternative to environmentally toxic and non-biodegradable synthetic insecticides. [ABSTRACT FROM AUTHOR]

The major loci for the large primary ribosomal RNA (rRNA) genes (35S rRNAs) exist as hundreds to thousands of tandem repeats in all organisms and dozens to hundreds in Drosophila. The highly repetitive nature of the ribosomal DNA (rDNA) makes it intrinsically unstable, and many conditions arise from the reduction in or magnification of copy number, but the conditions under which it does so remain unknown. By targeted DNA damage to the rDNA of the Y chromosome, we created and investigated a series of rDNA alleles. We found that complete loss of rDNA leads to lethality after the completion of embryogenesis, blocking larval molting and metamorphosis. We find that the resident retrotransposons— R1 and R2 —are regulated by active rDNA such that reduction in copy number derepresses these elements. Their expression is highest during the early first instar, when loss of rDNA is lethal. Regulation of R1 and R2 may be related to their structural arrangement within the rDNA , as we find they are clustered in the flanks of the nucleolus organizing region (NOR ; the cytological appearance of the rDNA). We assessed the complex nucleolar dominance relationship between X - and Y -linked rDNA using a histone H3.3–GFP reporter construct and incorporation at the NOR and found that dominance is controlled by rDNA copy number as at high multiplicity the Y -linked array is dominant, but at low multiplicity the X -linked array becomes derepressed. Finally, we found that multiple conditions that disrupt nucleolar dominance lead to increased rDNA magnification, suggesting that the phenomena of dominance and magnification are related, and a single mechanism may underlie and unify these two longstanding observations in Drosophila. [ABSTRACT FROM AUTHOR]

In the present work, laboratory assays were performed to appraise the biocontrol potential of Metarhabditis amsactae, a Rhabditid nematode, against fifth instar larvae of Corcyra cephalonica. Virulence was tested at four different doses, i.e. 20, 40, 80 and 160 IJs/L (infective juveniles per larva) and mortality was documented from 24 up to 96 h of nematode treatment. Concentration and time-based enhancement in larval mortality was observed with 100% mortality recorded at 160 IJs/L after 96 h. Even at 20 IJs/L concentration, 60% and 76.7% larval mortality was recorded after 72 and 96 h, respectively. At 80 IJs/L, the mortality increased from 33.3% at 48 h to more than 90% at 96 h of nematode inoculation. Whereas, at 40 IJs/L, it enhanced from 16.7% at 48 h to 83.3% at 96 h. The effect of M. amsactae infection on the immune system of C. cephalonica was also evaluated at a dose of 100 IJs/L after 12, 24, 36 and 48 h time intervals. Insect larvae displayed augmented levels of antioxidant and detoxifying enzymes. The peak activities of superoxide dismutase, catalase, ascorbate peroxidase, phenol oxidase and glutathione-S-transferase in infected larvae were observed after 36 h, whereas acetylcholine esterase levels peaked after 48 h, as compared to control. Malondialdehyde content was also increased post nematode infection. Thus the current study is the first report that depicts the significant bioefficacy and immunomodulatory effects of M. amsactae against C. cephalonica under laboratory conditions. However, further research to assess its efficiency under field conditions is required. [ABSTRACT FROM AUTHOR]

Simple Summary: Carotenoids are organic compounds with various important functions in animals. Most animals obtain their carotenoids only from their food. House flies are common pests with a worldwide distribution. Their larvae feed on organic matter that may vary substantially in its carotenoid composition. This study examines if the carotenoid composition in the body of adult house flies is related to the composition in the larval feed. House fly larvae were reared on diets that differed in carotenoid composition. HPLC analysis of the emerging adult flies indicates that the carotenoid composition in adult house flies was similar, but not identical, to the composition in the larval feed. These results suggest that carotenoid composition in adult flies may be used to estimate the composition in their natal habitat and therefore may be used to identify potential sources of house fly infestations. Also, the feed of house fly larvae, used for animal feed, should be carefully considered. Carotenoids are common and diverse organic compounds with various functional roles in animals. Except for certain aphids, mites, and gall midges, all animals only acquire necessary carotenoids through their diet. The house fly (Musca domestica) is a cosmopolitan pest insect that populates diverse habitats. Its larvae feed on organic substrates that may vary in carotenoid composition according to their specific content. We hypothesized that the carotenoid composition in the adult house fly's body would reflect the carotenoid composition in the larval feed. House fly larvae were reared on diets that differed in carotenoid composition. HPLC analysis of the emerging adult flies indicate that the carotenoid composition of adult house flies is related, but not identical, to the carotenoid composition in its natal substrate. These findings may be developed to help identify potential sources of house fly infestations. Also, it is recommended that rearing substrates of house fly larvae, used for animal feed, should be carefully considered. [ABSTRACT FROM AUTHOR]

BACKGROUND: Per- and polyfluoroalkyl Substances (PFAS) are synthetic chemicals widely detected in humans and the environment. Exposure to perfluorooctanesulfonic acid (PFOS) or perfluorohexanesulfonic acid (PFHxS) was previously shown to cause dark-phase hyperactivity in larval zebrafish. OBJECTIVES: The objective of this study was to elucidate the mechanism by which PFOS or PFHxS exposure caused hyperactivity in larval zebrafish. METHODS: Swimming behavior was assessed in 5-d postfertilization (dpf) larvae following developmental (1-4 dpf) or acute (5 dpf) exposure to 0.43-7.86μM PFOS, 7.87-120μ M PFHxS, or 0.4% dimethyl sulfoxide (DMSO). After developmental exposure and chemical washout at 4 dpf, behavior was also assessed at 5-8 dpf. RNA sequencing was used to identify differences in global gene expression to perform transcriptomic benchmark concentration-response (BMCT) modeling, and predict upstream regulators in PFOS- or PFHxS-exposed larvae. CRISPR/Cas9-based gene editing was used to knockdown peroxisome proliferator-activated receptors (ppars) pparaa/ab, pparda/db, or pparg at day 0. Knockdown crispants were exposed to 7.86μM PFOS or 0.4% DMSO from 1-4 dpf and behavior was assessed at 5 dpf. Coexposure with the ppard antagonist GSK3787 and PFOS was also performed RESULTS: Transient dark-phase hyperactivity occurred following developmental or acute exposure to PFOS or PFHxS, relative to the DMSO control. In contrast, visual startle response (VSR) hyperactivity only occurred following developmental exposure and was irreversible up to 8 dpf. Similar global transcriptomic profiles, BMCT estimates, and enriched functions were observed in PFOS- and PFHxS-exposed larvae, and ppars were identified as putative upstream regulators. Knockdown of pparda/db, but not pparaa/ab or pparg, blunted PFOS-dependent VSR hyperactivity to control levels. This finding was confirmed via antagonism of ppard in PFOS-exposed larvae. DISCUSSION: This work identifies a novel adverse outcome pathway for VSR hyperactivity in larval zebrafish. We demonstrate that developmental, but not acute, exposure to PFOS triggered persistent VSR hyperactivity that required ppard function. [ABSTRACT FROM AUTHOR]

Disease free layings of silkworm breed, DUN22 were procured, incubated, brushed and reared upto 3rd instar enmass following the standard rearing method. Just after 3rd moult, the experiment was laid down into four treatments (T1-T4) as per the experimental design. Five replications of 100 larvae in each treatment were maintained and data with regard to 5th instar and total larval duration, diseases incidence, yield and pupation rate were recorded and analysed statistically. Perusal of data revealed significant difference in afore mentioned economic traits and therefore concluded that Bombyx mori Nuclear Polyhedrosis Virus (BmNPV) spreads infection secondarily and also had negative impact on the silkworm, B. mori. [ABSTRACT FROM AUTHOR]

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Background: Cystic echinococcosis (CE) is one of the most important neglected diseases and a public health concern worldwide. Due to the poor efficacy of current drugs, novel therapeutic approaches are urgently needed. Objectives: This study evaluated the scolicidal effects of the hydroalcoholic extract of Urtica dioica and the chloroformic extract of Pyrus boissieriana on protoscoleces of CE cysts. Methods: Protoscoleces were aseptically aspirated from the livers of sheep naturally infected with CE cysts. To assess the scolicidal effect of these herbal extracts, various concentrations of both extracts (5, 10, 20, 40, and 80 mg/mL) were added to a suspension of 3 x 10³ washed protoscoleces. After 10, 20, 30, 60, and 120 minutes of exposure, eosin stain was added to each tube, and the viability of protoscoleces was evaluated by flame cell motility under a light microscope, as well as impermeability to 0.1% eosin solution. All experiments were performed in triplicate. Results: The scolicidal effects of U. dioica extracts at a concentration of 80 mg/mL were 81% and 89% after 60 and 120 minutes of exposure, respectively. Almost the same results were obtained for P. boissieriana extracts at a concentration of 80 mg/mL (81.33% and 89% after 60 and 120 minutes of exposure, respectively), which were significantly different from the negative control group (P < 0.001). However, the extract of U. dioica exhibited stronger scolicidal effects compared to the extract of P. boissieriana at concentrations lower than 40 mg/mL (P < 0.001). Conclusions: The present findings indicate that both U. dioica and P. boissieriana extracts possess high protoscolicidal activities and could be used as alternative natural medicines in the treatment of CE. However, further studies are required to verify these findings through assessment in animal models and clinical subjects. [ABSTRACT FROM AUTHOR]

Anxiety disorders are the most prevalent psychiatric disorders, exhibiting strong female bias. Clinical studies implicate declining estradiol levels in the exacerbation of anxiety symptoms in the premenstrual phase of the menstrual cycle. This study aimed to simulate estradiol fluctuation-linked anxiety behavior in larval zebrafish, using an estradiol treatment withdrawal model. Contrary to model aims, estradiol treatment withdrawal decreased both basal activity and anxiety-like hyperlocomotion (ANOVA main effect of dose, P < 0.0001 and P < 0.01, respectively) in the light/dark transition test. The accuracy of the estradiol washout model was not improved by longer durations of treatment or withdrawal. Basal activity was slightly altered by supraphysiological concentrations of WAY-200070 in the absence of added estradiol. Estrogen receptor (ER) β expression was not upregulated in larvae exposed to physiologically relevant, low concentrations of estradiol. Longer exposure to low concentrations of estradiol increased antioxidant capacity (P < 0.01). In addition, acute exposure to low concentrations of estradiol increased basal activity. Data suggest that in the current models, estradiol-associated altered activity levels were linked to more favorable redox status, rather than reflecting altered anxiety levels. As such, it is recommended that zebrafish larval behavioral analysis be conducted in parallel with mechanistic studies such as redox indicators, for investigations focused on ER signaling. [ABSTRACT FROM AUTHOR]

Candida albicans (C. albicans), a microbe commonly isolated from Candida vaginitis patients with vaginal tract infections, transforms from yeast to hyphae and produces many toxins, adhesins, and invasins, as well as C. albicans biofilms resistant to antifungal antibiotic treatment. Effective agents against this pathogen are urgently needed. Antimicrobial peptides (AMPs) have been used to cure inflammation and infectious diseases. In this study, we isolated whole housefly larvae insect SVWC peptide 1 (WHIS1), a novel insect single vonWillebrand factor C-domain protein (SVWC) peptide from whole housefly larvae. The expression pattern of WHIS1 showed a response to the stimulation of C. albicans. In contrast to other SVWC members, which function as antiviral peptides, interferon (IFN) analogs or pathogen recognition receptors (PRRs), which are the prokaryotically expressed MdWHIS1 protein, inhibit the growth of C. albicans. Eukaryotic heterologous expression of WHIS1 inhibited C. albicans invasion into A549 and HeLa cells. The heterologous expression of WHIS1 clearly inhibited hyphal formation both extracellularly and intracellularly. Furthermore, themechanismof WHIS1 has demonstrated that it downregulates all key hyphal formation factors (ALS1, ALS3, ALS5, ECE1, HWP1, HGC1, EFG1, and ZAP1) both extracellularly and intracellularly. These data showed that heterologously expressed WHIS1 inhibits C. albicans invasion into epithelial cells by affecting hyphal formation and adhesion factor-related gene expression. These findings provide new potential drug candidates for treating C. albicans infection. [ABSTRACT FROM AUTHOR]

Female herbivorous insects are expected to oviposit on the host plant providing the best performance of the offspring. However, in some insects the larvae are mobile and are not totally dependent on the mother's choice. They can change host plant when conditions for development or exposure to natural enemies vary between individual plants within a patch. Here we study larval migration and preference between two host plants, cotton and alfalfa, in the Egyptian leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). Previous studies have shown that although larval performance is better on cotton, females prefer to oviposit on alfalfa, and this preference corresponds to a higher survival of the eggs. In this study, S. littoralis larvae showed directed movement between host plants and were found to prefer alfalfa over cotton in field test in Egypt, as well as in laboratory selection of feeding site assays. To determine effects by natural enemies, the parasitism rates and various life‐history traits were measured for one larval parasitoid, Microplitis rufiventris Kokujev (Hymenoptera: Braconidae), on several host plants including cotton and clover (equivalent host plant to alfalfa). Overall, parasitism was higher on cotton and parasitoid performance (cocoon mass, adult longevity, and female egg load) was better on cotton compared to clover. This fact suggests an enemy‐free space on clover and alfalfa, as parasitism rate is higher on cotton, and the parasitoid performance is also better on cotton‐fed larvae. [ABSTRACT FROM AUTHOR]

Entomopathogenic fungi have been considered potential biological control agents against the fall armyworm Spodoptera frugiperda (J. E. Smith), the world's most important pest of maize. In this study, we evaluated the natural infection, molecular characteristics, and biological activity of Metarhizium rileyi (Farlow) isolated from S. frugiperda larvae of this insect, collected from maize crops in five Mexican locations. Natural infection ranged from 23% to 90% across all locations analyzed. Twenty-four isolates were evaluated on S. frugiperda second instars at a concentration of 1.0 × 108 conidia/mL, causing 70% to 98.7% mortality and 60.5% to 98.7% sporulation. Isolates T9-21, Z30-21, PP48-21, and L8-22 were selected to determine their phylogenetic relationships by β-tubulin gene analysis and to compare median lethal concentration (CL50), median lethal time (LT50), and larval survival. These isolates were grouped into three clades. The T9-21, PP48-21, and J10-22 isolates were closely related (clade A), but phylogenetically distant from Z30-21 (clade B) and L8-22 (clade C) isolates. These genetic differences were not always reflected in their pathogenicity characteristics since no differences were observed among the LC50 values. Furthermore, isolates T9-21, J10-22, and L8-22 were the fastest to kill S. frugiperda larvae, causing lower survival rates. We conclude that native M. rileyi isolates represent an important alternative for the biocontrol of S. frugiperda. [ABSTRACT FROM AUTHOR]

Simple Summary: Mass outbreaks of the pine sawfly Diprion pini can cause severe damage to pine forests. The larvae of this herbivorous insect feed selectively on the needles of pine trees, notably Pinus sylvestris. During mass outbreak periods, the females release large amounts of sex pheromones. A prior study revealed that the survival rate of sawfly eggs laid on pheromone-exposed pine needles was lower than that of eggs on unexposed pine. In our study, we found that D. pini females avoided oviposition on pheromone-exposed pine, possibly as a counter-adaptation to the enhanced defenses of previously pheromone-exposed trees against sawfly eggs. The females only discriminated between pheromone-exposed and unexposed trees when they had the chance to touch the needles, but not when exposed to the odor of these types of trees. However, the performance of larvae did not significantly differ on pheromone-exposed and unexposed trees. These results underscore the complexity of the chemical ecology of sawfly–pine interactions and highlight the nuanced roles that pheromones play in shaping the relationships between herbivores and their host plants. Insect pheromones have been intensively studied with respect to their role in insect communication. However, scarce knowledge is available on the impact of pheromones on plant responses, and how these in turn affect herbivorous insects. A previous study showed that exposure of pine (Pinus sylvestris) to the sex pheromones of the pine sawfly Diprion pini results in enhanced defenses against the eggs of this sawfly; the egg survival rate on pheromone-exposed pine needles was lower than that on unexposed pine. The long-lasting common evolutionary history of D. pini and P. sylvestris suggests that D. pini has developed counter-adaptations to these pine responses. Here, we investigated by behavioral assays how D. pini copes with the defenses of pheromone-exposed pine. The sawfly females did not discriminate between the odor of pheromone-exposed and unexposed pine. However, when they had the chance to contact the trees, more unexposed than pheromone-exposed trees received eggs. The exposure of pine to the pheromones did not affect the performance of larvae and their pupation success. Our findings indicate that the effects that responses of pine to D. pini sex pheromones exert on the sawfly eggs and sawfly oviposition behavior do not extend to effects on the larvae. [ABSTRACT FROM AUTHOR]