126 results on '"Wenfeng, Luo"'
Search Results
2. circICMT upregulates and suppresses the malignant behavior of bladder cancer
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Xin Luo, FangMei Xie, Guoqiang Qin, Ge Zou, Xu Lu, Chaofeng Zhang, Zeping Han, Ying Zhao, Xiaoyu Song, WenFeng Luo, Yongsheng Li, JinHua He, and Jian Shen
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Bladder cancer ,circRNA ,circICMT ,ceRNA ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: Circular RNA (circRNA) is a new type of endogenous single-stranded RNA with a covalently closed circular structure. Increasing evidence shows that circRNA plays an important role in regulating gene expression in tumors. circICMT is a circular RNA produced by the ICMT gene. Currently, the molecular function of circICMT in bladder cancer remains unclear. Method: Differentially expressed circRNAs were identified from RNA sequencing data and circICMT was identified as a new candidate circRNA. qRT-PCR and sanger sequencing were used to detect the expression of circICMT in bladder cancer tissue specimens. Stable cell lines overexpressing and knocking down circICMT were constructed to explore the effect of circICMT on bladder cancer cells. Its biological effects were detected through wound healing experiments, colony formation experiments, CCK-8 experiments and xenogeneic tumorigenesis experiments. Result: This study found that circICMT was significantly upregulated in bladder cancer tissue specimens. Overexpression of circICMT can inhibit cell migration, proliferation and colony formation ability, while knockdown of circICMT promotes the malignant phenotype of bladder cancer cells. Bioinformatics predictions have found that circICMT can bind to a variety of miRNAs and RBPs and may form a complex regulatory network to regulate the progression of bladder cancer. Conclusion: circICMT is significantly highly expressed in bladder cancer, and intervening circICMT expression affects the malignant phenotype of bladder cancer cells in vivo and in vitro, which may provide potential biomarkers and therapeutic targets for the management of bladder cancer.
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- 2025
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3. A case report of outer hair-root sheath cancer
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Yuteng Huang, Zhenju Tang, Wenfeng Luo, and Weiliang Wang
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Surgery ,RD1-811 - Published
- 2025
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4. Effect of konjac glucomannan on gut microbiota from hyperuricemia subjects in vitro: fermentation characteristics and inhibitory xanthine oxidase activity
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Jie Deng, Kai Zhou, Caimin Feng, Yilu Bao, Zhiming Zhang, Wenfeng Luo, and Meiying Li
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konjac glucomannan ,short-chain fatty acids ,xanthine oxidase activity ,hyperuricemia ,uric acid ,Nutrition. Foods and food supply ,TX341-641 - Abstract
BackgroundThe disorder of uric acid metabolism is closely associated with gut microbiota and short-chain fatty acids (SCFAs) dysregulation, but the biological mechanism is unclear, limiting the development of uric acid-lowering active polysaccharides. Konjac glucomannan (KGM) could attenuate metabolic disturbance of uric acid and modulate the gut microbiota. However, the relationship between uric acid metabolism and gut microbiota is still unknown.MethodsIn this study, The fecal samples were provided by healthy volunteers and hyperuricemia (HUA) patients. Fecal samples from healthy volunteers was regarded as the NOR group. Similarly, 10% HUA fecal suspension was named as the HUA group. Then, fecal supernatant was inoculated into a growth basal medium containing glucose or KGM, and healthy fecal samples were designated as the NOR-GLU and NOR-KGM groups, while HUA fecal samples were designated as the HUA-GLU and HUA-KGM groups. All samples were cultured in an anaerobic bag system. After fermentation for 24 h, the samples were collected for further analysis of composition of intestinal microbiota, SCFAs concentration and XOD enzyme activity.ResultsThe results showed that KGM could be utilized and degraded by the gut microbiota from HUA subjects, and it could modulate the composition and structure of their HUA gut microbiota to more closely resemble that of a healthy group. In addition, KGM showed a superior modulated effect on HUA gut microbiota by increasing Megasphaera, Faecalibacterium, Lachnoclostridium, Lachnospiraceae, Anaerostipes, and Ruminococcus levels and decreasing Butyricicoccus, Eisenbergiella, and Enterococcus levels. Furthermore, the fermentation solution of KGM showed an inhibitory effect on xanthine oxidase (XOD) enzyme activity, which might be due to metabolites such as SCFAs.ConclusionIn conclusion, the effect of KGM on hyperuricemia subjects was investigated based on the gut microbiota in vitro. In the present study. It was found that KGM could be metabolized into SCFAs by HUA gut microbiota. Furthermore, KGM could modulate the structure of HUA gut microbiota. At the genus level, KGM could decrease the relative abundances of Butyricicoccus, Eisenbergiella, and Enterococcus, while Lachnoclostridium and Lachnospiraceae in HUA gut microbiota were significantly increased by the addition of KGM. The metabolites of gut microbiota, such as SCFAs, might be responsible for the inhibition of XOD activity. Thus, KGM exhibited a superior probiotic function on the HUA gut microbiota, which is expected as a promising candidate for remodeling the HUA gut microbiota.
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- 2024
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5. Comprehensive analysis and prognostic assessment of senescence-associated genes in bladder cancer
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Ruilin Yang, Jieling He, Wenfeng Luo, Renyang Xiang, Ge Zou, Xintao Zhang, Huang Liu, and Junhong Deng
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Bladder cancer ,Senescence-associated genes ,Transcriptomic analysis ,Prognosis ,Immunotherapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background The prevalence and mortality of bladder cancer (BLCA) present a significant medical challenge. While the function of senescence-related genes in tumor development is recognized, their prognostic significance in BLCA has not been thoroughly explored. Methods BLCA transcriptome datasets were sourced from the TCGA and GEO repositories. Gene groupings were determined through differential gene expression and non-negative matrix factorization (NMF) methodologies. Key senescence-linked genes were isolated using singular and multivariate Cox regression analyses, combined with lasso regression. Validation was undertaken with GEO database information. Predictive models, or nomograms, were developed by merging risk metrics with clinical records, and their efficacy was gauged using ROC curve methodologies. The immune response’s dependency on the risk metric was assessed through the immune phenomenon score (IPS). Additionally, we estimated IC50 metrics for potential chemotherapeutic agents. Results Reviewing 406 neoplastic and 19 standard tissue specimens from the TCGA repository facilitated the bifurcation of subjects into two unique clusters (C1 and C2) according to senescence-related gene expression. After a stringent statistical evaluation, a set of ten pivotal genes was discerned and applied for risk stratification. Validity tests for the devised nomograms in forecasting 1, 3, and 5-year survival probabilities for BLCA patients were executed via ROC and calibration plots. IC50 estimations highlighted a heightened responsiveness in the low-risk category to agents like cisplatin, cyclopamine, and sorafenib. Conclusions In summation, our research emphasizes the prospective utility of risk assessments rooted in senescence-related gene signatures for enhancing BLCA clinical oversight.
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- 2024
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6. The role of protein post-translational modifications in prostate cancer
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Yinghui Hao, Chenqiong Gu, Wenfeng Luo, Jian Shen, Fangmei Xie, Ying Zhao, Xiaoyu Song, Zeping Han, and Jinhua He
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Protein post-translational modification ,Prostate cancer ,Diagnosis ,Treatment ,Prognosis ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Involving addition of chemical groups or protein units to specific residues of the target protein, post-translational modifications (PTMs) alter the charge, hydrophobicity, and conformation of a protein, which in turn influences protein function, protein–protein interaction, and protein aggregation. These alterations, which include phosphorylation, glycosylation, ubiquitination, methylation, acetylation, lipidation, and lactylation, are significant biological events in the development of cancer, and play vital roles in numerous biological processes. The processes behind essential functions, the screening of clinical illness signs, and the identification of therapeutic targets all depend heavily on further research into the PTMs. This review outlines the influence of several PTM types on prostate cancer (PCa) diagnosis, therapy, and prognosis in an effort to shed fresh light on the molecular causes and progression of the disease.
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- 2024
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7. Identification and validation of a novel glycolysis-related ceRNA network for sepsis-induced cardiomyopathy
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Lulu Cheng, Jiabin Liang, Fangmei Xie, Zeping Han, Wenfeng Luo, Hanwei Chen, and Jinhua He
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sepsis-induced cardiomyopathy ,glycolysis ,ceRNA network ,bioinformatics analysis ,IER3 ,Medicine (General) ,R5-920 - Abstract
PurposeSepsis-induced cardiomyopathy (SIC) is a major life-threatening condition in critically infected patients. Early diagnosis and intervention are important to improve patient prognosis. Recognizing the pivotal involvement of the glycolytic pathway in SIC, this study aims to establish a glycolysis-related ceRNA network and explore novel diagnostic avenues.Materials and methodsSIC-related datasets were carefully filtered from the GEO database. CytoHubba was used to identify differentially expressed genes (DEGs) associated with glycolysis. A predictive method was then used to construct an lncRNA-miRNA-mRNA network. Dual-luciferase reporter assays validated gene interactions, and the specificity of this ceRNA network was confirmed in peripheral blood mononuclear cells (PBMCs) from SIC patients. Logistic analysis was used to examine the correlation between the ceRNA network and SIC. Diagnostic potential was assessed using receiver operating characteristic (ROC) curves, and correlation analysis investigated any associations between gene expression and clinical indicators.ResultsIER3 was identified as glycolysis-related DEG in SIC, and a ceRNA network (SNHG17/miR-214-3p/IER3) was established by prediction. Dual luciferase reporter gene assay confirmed the presence of mutual binding between IER3, miR-214-3p and SNHG17. RT-qPCR verified the specific expression of this ceRNA network in SIC patients. Multivariate logistic analysis established the correlation between the ceRNA network and SIC. ROC analysis demonstrated its high diagnostic specificity (AUC > 0.8). Correlation analysis revealed a negative association between IER3 expression and oxygenation index in SIC patients (p
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- 2024
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8. Non-coding RNAs are involved in tumor cell death and affect tumorigenesis, progression, and treatment: a systematic review
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Zeping Han, Wenfeng Luo, Jian Shen, Fangmei Xie, Jinggen Luo, Xiang Yang, Ting Pang, Yubing Lv, Yuguang Li, Xingkui Tang, and Jinhua He
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non-coding RNAs ,autophagy, ferroptosis ,pyroptosis ,tumorigenesis ,tumor progression ,treatment ,Biology (General) ,QH301-705.5 - Abstract
Cell death is ubiquitous during development and throughout life and is a genetically determined active and ordered process that plays a crucial role in regulating homeostasis. Cell death includes regulated cell death and non-programmed cell death, and the common types of regulatory cell death are necrosis, apoptosis, necroptosis, autophagy, ferroptosis, and pyroptosis. Apoptosis, Necrosis and necroptosis are more common than autophagy, ferroptosis and pyroptosis among cell death. Non-coding RNAs are regulatory RNA molecules that do not encode proteins and include mainly microRNAs, long non-coding RNAs, and circular RNAs. Non-coding RNAs can act as oncogenes and tumor suppressor genes, with significant effects on tumor occurrence and development, and they can also regulate tumor cell autophagy, ferroptosis, and pyroptosis at the transcriptional or post-transcriptional level. This paper reviews the recent research progress on the effects of the non-coding RNAs involved in autophagy, ferroptosis, and pyroptosis on tumorigenesis, tumor development, and treatment, and looks forward to the future direction of this field, which will help to elucidate the molecular mechanisms of tumorigenesis and tumor development, as well as provide a new vision for the treatment of tumors.
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- 2024
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9. Integrating Single-Cell and Spatial Transcriptomics to Uncover and Elucidate GP73-Mediated Pro-Angiogenic Regulatory Networks in Hepatocellular Carcinoma
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Jiazhou Ye, Xing Gao, Xi Huang, Shilin Huang, Dandan Zeng, Wenfeng Luo, Can Zeng, Cheng Lu, Lu Lu, Hongyang Huang, Kaixiang Mo, Julu Huang, Shizhou Li, Minchao Tang, Tianzhun Wu, Rongyun Mai, Min Luo, Mingzhi Xie, Shan Wang, Yongqiang Li, Yan Lin, and Rong Liang
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Science - Abstract
Hepatocellular carcinoma (HCC) was characterized as being hypervascular. In the present study, we generated a single-cell spatial transcriptomic landscape of the vasculogenic etiology of HCC and illustrated overexpressed Golgi phosphoprotein 73 (GP73) HCC cells exerting cellular communication with vascular endothelial cells with high pro-angiogenesis potential via multiple receptor–ligand interactions in the process of tumor vascular development. Specifically, we uncovered an interactive GP73-mediated regulatory network coordinated with c-Myc, lactate, Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway, and endoplasmic reticulum stress (ERS) signals in HCC cells and elucidated its pro-angiogenic roles in vitro and in vivo. Mechanistically, we found that GP73, the pivotal hub gene, was activated by histone lactylation and c-Myc, which stimulated the phosphorylation of downstream STAT3 by directly binding STAT3 and simultaneously enhancing glucose-regulated protein 78 (GRP78)-induced ERS. STAT3 potentiates GP73-mediated pro-angiogenic functions. Clinically, serum GP73 levels were positively correlated with HCC response to anti-angiogenic regimens and were essential for a prognostic nomogram showing good predictive performance for determining 6-month and 1-year survival in patients with HCC treated with anti-angiogenic therapy. Taken together, the aforementioned data characterized the pro-angiogenic roles and mechanisms of a GP73-mediated network and proved that GP73 is a crucial tumor angiogenesis niche gene with favorable anti-angiogenic potential in the treatment of HCC.
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- 2024
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10. Multipole interference and optical sensing based on the bound states in the continuum formed in dimer metasurfaces
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Shuyuan Lv, Haifeng Xu, Wenfeng Luo, Fei Hu, and Yating Dang
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Bound state in the continuum ,Dielectric metasurface ,Mie resonance ,Multipole interference ,Dimer ,Physics ,QC1-999 - Abstract
The all-dielectric metasurface supporting BIC based on dimer structures is proposed with the rectangular holes etched in amorphous silicon thin films. By introducing perturbations of the rotation angle of the rectangular hole as well as the difference in length and width, quasi-BIC modes in the near-infrared band can be created. Meanwhile, the research found that two different perturbations display different tuning abilities on the Fano resonance induced by the quasi-BIC. This paper reveals that the formation of the BIC mode originates from the interference between competing multipole moments, which ultimately leads to the emergence of a pair of reverse parallel electric dipoles. It explains the mismatch between the macroscopic response of the structure under the influence of competing multipole moments and the results of the multipole expansion. From the perspective of the influence of asymmetry on multipole moments, we explain the resulting radiation sources. The study demonstrates that the proposed dimer metasurface can support optical sensing under both x-polarization and circular polarization excitation, and it is observed that different perturbations have different abilities to modulate the sensitivity, which has great potential for device performance optimization and precise modulation.
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- 2023
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11. circMSH3 is a potential biomarker for the diagnosis of colorectal cancer and affects the distant metastasis of colorectal cancer
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Jian Shen, Yu Min, Jingen Luo, Xingkui Tang, Zeping Han, Wenfeng Luo, Fangmei Xie, Mingrong Cao, Taicheng Zhou, and Jinhua He
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Colorectal cancer ,Hsa_circ_0003761 ,CircMSH3 ,RNA-protein pull-down ,MiR-1276 ,MiR-942-5p ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Objectives To identify the most significantly differentially expressed circular RNAs (circRNAs) in colorectal cancer (CRC) tissues in terms of their expression levels and circularity, and to analyze the relationship between their expression levels and the clinical characteristics of patients. Methods circRNA RNA-seq technology was used to screen differentially expressed circRNAs in CRC. Sanger sequencing was used to identify circRNA back-splice junction sites. The relative expression levels of hsa_circ_0003761 (circMSH3) in CRC tissues and cell lines were detected by quantitative real-time fluorescence PCR technology. An RNA-protein pull-down assay was used to detect protein binding to circRNAs. Dual-luciferase reporter gene vectors were constructed to verify that circRNAs bind to microRNAs. Results Four hundred twenty circRNAs were found to be upregulated, and 616 circRNAs were downregulated. circMSH3 was derived from the MutS homolog 3 (MSH3) gene and was formed by a loop of exons 9, 10, 11, and 12. In 110 pairs of CRC and adjacent tissues, circMSH3 expression was 4.487-fold higher in CRC tissues. circMSH3 was also highly expressed in the HT-29 and LOVO CRC cell lines. The expression level of circMSH3 was associated with distant metastasis in CRC patients (P = 0.043); the area under the curve (AUC) of circMSH3 for CRC diagnosis was 0.75, with a sensitivity and specificity of 70.9% and 66.4%, respectively. circMSH3 could bind to a variety of proteins, mainly those involved in RNA transcription, splicing, cell cycle, and cell junctions. Furthermore, circMSH3 could bind to miR-1276, miR-942-5p, and miR-409-3p. Conclusion circMSH3 is a potential biomarker for the diagnosis of CRC and affects the distant metastasis of CRC. Multiple RNA-binding protein binds to circMSH3, and circMSH3 binds to miR-1276, miR-942-5p, and miR-409-3p, thereby affecting the expression of circMSH3.
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- 2023
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12. Adversarial Decoupling for Weakly Supervised Semantic Segmentation.
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Guoying Sun, Meng Yang 0001, and Wenfeng Luo
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- 2021
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13. Serum organic acid metabolites can be used as potential biomarkers to identify prostatitis, benign prostatic hyperplasia, and prostate cancer
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Jinhua He, Zeping Han, Wenfeng Luo, Jian Shen, Fangmei Xie, Liyin Liao, Ge Zou, Xin Luo, Zhonghui Guo, Yuguang Li, Jianhao Li, and Hanwei Chen
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organic acid metabolites ,LC-MS ,prostatitis ,benign prostatic hyperplasia ,prostate cancer ,Immunologic diseases. Allergy ,RC581-607 - Abstract
BackgroundNoninvasive methods for the early identify diagnosis of prostatitis, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) are current clinical challenges.MethodsThe serum metabolites of 20 healthy individuals and patients with prostatitis, BPH, or PCa were identified using untargeted liquid chromatography-mass spectrometry (LC-MS). In addition, targeted LC-MS was used to verify the organic acid metabolites in the serum of a validation cohort.ResultsOrganic acid metabolites had good sensitivity and specificity in differentiating prostatitis, BPH, and PCa. Three diagnostic models identified patients with PROSTATITIS: phenyllactic acid (area under the curve [AUC]=0.773), pyroglutamic acid (AUC=0.725), and pantothenic acid (AUC=0.721). Three diagnostic models identified BPH: citric acid (AUC=0.859), malic acid (AUC=0.820), and D-glucuronic acid (AUC=0.810). Four diagnostic models identified PCa: 3-hydroxy-3-methylglutaric acid (AUC=0.804), citric acid (AUC=0.918), malic acid (AUC=0.862), and phenyllactic acid (AUC=0.713). Two diagnostic models distinguished BPH from PCa: phenyllactic acid (AUC=0.769) and pyroglutamic acid (AUC=0.761). Three diagnostic models distinguished benign BPH from PROSTATITIS: citric acid (AUC=0.842), ethylmalonic acid (AUC=0.814), and hippuric acid (AUC=0.733). Six diagnostic models distinguished BPH from prostatitis: citric acid (AUC=0.926), pyroglutamic acid (AUC=0.864), phenyllactic acid (AUC=0.850), ethylmalonic acid (AUC=0.843), 3-hydroxy-3-methylglutaric acid (AUC=0.817), and hippuric acid (AUC=0.791). Three diagnostic models distinguished PCa patients with PROSTATITISA < 4.0 ng/mL from those with PSA > 4.0 ng/mL: 5-hydromethyl-2-furoic acid (AUC=0.749), ethylmalonic acid (AUC=0.750), and pyroglutamic acid (AUC=0.929). Conclusions: These results suggest that serum organic acid metabolites can be used as biomarkers to differentiate prostatitis, BPH, and PCa.
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- 2023
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14. circSPECC1 Promotes Proliferation and Migration of LNCaP Prostate Cancer Cells by Affecting Their Epithelial-Mesenchymal Transition
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Fangmei Xie, Jian Shen, Zeping Han, Wenfeng Luo, Liyin Liao, and Jinhua He
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Objective. To determine the effects of circSPECC1 (hsa_circ_0000745) on the proliferation and migration of LNCaP prostate cancer cells and to explore the potential molecular mechanism. Methods. Stable circSPECC1 shRNA-expressing and circSPECC1-overexpressing LNCaP cell lines were constructed, and relative gene expression levels were determined by RT-PCR. MTT and clonogenic assays were used to assess proliferative ability while a scratch test was used to analyze cell migration. Western blotting was used to determine protein expression levels. The effects of circSPECC1 on the proliferation of LNCaP prostate cancer cells were observed in vivo. Results. circSPECC1 was found to be derived from the SPECC1 (sperm antigen with calponin homology and coiled-coil domains 1) parent gene and to form a loop. Overexpression of circSPECC1 promoted the proliferation and migration of the LNCaP cells, whereas decreased expression of circSPECC1 inhibited these properties. Overexpression of circSPECC1 promoted the expression of MMP-2, MMP-9, VEGF, vimentin, and N-cad but downregulated the expression of E-cad. Decreased expression of circSPECC1 inhibited the expression of MMP-2, MMP-9, VEGF, vimentin, and N-cad but increased the expression of E-cad. Conclusion. circSPECC1 promotes cell proliferation and migration by affecting the epithelial-mesenchymal transition of LNCaP prostate cancer cells.
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- 2023
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15. Progress in the application of body fluid and tissue level mRNAs-non-coding RNAs for the early diagnosis and prognostic evaluation of systemic lupus erythematosus
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Jiabin Liang, Fangmei Xie, Jie Feng, Chen Huang, Jian Shen, Zeping Han, Wenfeng Luo, Jinhua He, and Hanwei Chen
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systemic lupus erythematosus ,biomarker ,mRNA ,microRNA ,lncRNA ,snoRNA ,Immunologic diseases. Allergy ,RC581-607 - Abstract
The diagnosis and differential classification of systemic lupus erythematosus (SLE) is difficult, especially in patients with early-onset SLE who are susceptible to systemic multi-organ damage and serious complications and have difficulties in individualized treatment. At present, diagnosis is based mainly on clinical manifestations and the detection of serological antinuclear antibodies. The pathogenesis of SLE involves multiple factors, is clinically heterogeneous, and lacks specific biomarkers. Therefore, it is necessary to identify new biomarkers for the diagnosis and subtype classification of SLE. Non-coding RNAs (ncRNAs) are composed of microRNAs, long non-coding RNAs, small nucleolar RNAs, circular RNAs, and transfer RNAs. They play an important role in the occurrence and development of diseases and are used widely in the early diagnosis and prognosis of autoimmune diseases. In this review, we focus on the research progress in the diagnosis and prognostic assessment of SLE using humoral to tissue level ncRNAs.
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- 2022
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16. Study of the correlation between the expression levels of IL-6 and TNF-α, and the severity of acne in acne patients
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Huifang MO, Minhua ZHONG, Kaiyu CHU, Wenfeng LUO, Weihong ZHONG, Yongjun TANG, Yijun CHEN, and Haisheng CHEN
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acne ,il-6 ,tnf-α ,Dermatology ,RL1-803 - Abstract
Objective: To assess the correlation of IL-6 and TNF-α levels and the severity of acne. Methods: Blood samples were collected from 30 normal controls and 114 patients with acne vulgaris before and after 4-week treatments. According to the severity of acne, patients were divided into mild, moderate and severe groups. Chemilunescence was used to measure expression levels of IL-6 and TNF-α. Rusults: The expression levels of IL-6, but not TNF-α, in patients with acne were significantly higher than that in the controls (for IL-6, P0.05). Expression levels of IL-6 and TNF-α were not correlated with the severity of acne (both P>0.05). Conclusions: IL-6 is involved in the inflammatory response in acne, but cannot serve as an indicator of disease severity. Further study is needed to determine the relationship between TNF-α and acne.
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- 2021
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17. Erasing Integrated Learning: A Simple Yet Effective Approach for Weakly Supervised Object Localization.
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Jinjie Mai, Meng Yang 0001, and Wenfeng Luo
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- 2020
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18. Semi-supervised Semantic Segmentation via Strong-Weak Dual-Branch Network.
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Wenfeng Luo and Meng Yang 0001
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- 2020
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19. Learning Saliency-Free Model with Generic Features for Weakly-Supervised Semantic Segmentation.
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Wenfeng Luo and Meng Yang 0001
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- 2020
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20. Research progress on application of single-cell TCR/BCR sequencing technology to the tumor immune microenvironment, autoimmune diseases, and infectious diseases
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Jinhua He, Jian Shen, Wenfeng Luo, Zeping Han, Fangmei Xie, Ting Pang, Liyin Liao, Zhonghui Guo, Jianhao Li, Yuguang Li, and Hanwei Chen
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single-cell TCR/BCR sequencing ,autoimmune diseases ,infectious diseases ,Chronic inflammatory diseases ,Tumor immune microenvironment ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Single-cell omics is the profiling of individual cells through sequencing and other technologies including high-throughput analysis for single-cell resolution, cell classification, and identification as well as time series analyses. Unlike multicellular studies, single-cell omics overcomes the problem of cellular heterogeneity. It provides new methods and perspectives for in-depth analyses of the behavior and mechanism of individual cells in the cell population and their relationship with the body, and plays an important role in basic research and precision medicine. Single-cell sequencing technologies mainly include single-cell transcriptome sequencing, single-cell assay for transposase accessible chromatin with high-throughput sequencing, single-cell immune profiling (single-cell T-cell receptor [TCR]/B-cell receptor [BCR] sequencing), and single-cell transcriptomics. Single-cell TCR/BCR sequencing can be used to obtain a large amount of single-cell gene expression and immunomics data at one time, and combined with transcriptome sequencing and TCR/BCR diversity data, can resolve immune cell heterogeneity. This paper summarizes the progress in applying single-cell TCR/BCR sequencing technology to the tumor immune microenvironment, autoimmune diseases, infectious diseases, immunotherapy, and chronic inflammatory diseases, and discusses its shortcomings and prospects for future application.
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- 2022
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21. The Role of Liquid Biopsy Analytes in Diagnosis, Treatment and Prognosis of Colorectal Cancer
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JinHua He, NaiTe Xi, ZePing Han, WenFeng Luo, Jian Shen, ShengBo Wang, JianHao Li, ZhongHui Guo, and HanWei Cheng
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colorectal cancer ,circulating tumor cell ,circulating tumor DNA ,circulating tumor miRNA ,exosomes ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Colorectal cancer (CRC) is one of the most common malignant tumors of the digestive tract worldwide and is a serious threat to human life and health. CRC occurs and develops in a multi-step, multi-stage, and multi-gene process, in which abnormal gene expression plays an important role. CRC is currently diagnosed via endoscopy combined with tissue biopsy. Compared with tissue biopsy, liquid biopsy technology has received increasingly more attention and applications in the field of molecular detection due to its non-invasive, safe, comprehensive, and real-time dynamic nature. This review article discusses the application and limitations of current liquid biopsy analytes in the diagnosis, treatment, and prognosis of CRC, as well as directions for their future development.
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- 2022
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22. Circular RNAs and Their Role in Exosomes
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Zeping Han, Huafang Chen, Zhonghui Guo, Jian Shen, Wenfeng Luo, Fangmei Xie, Yu Wan, Shengbo Wang, Jianhao Li, and Jinhua He
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non-coding RNA (ncRNA) ,circRNA ,exosome ,exosomal circRNAs ,disease ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
As a novel class of endogenous non-coding RNAs discovered in recent years, circular RNAs (circRNAs) are highly conserved and stable covalently closed ring structures with no 5′-end cap or 3′-end poly(A) tail. CircRNAs are formed by reverse splicing, mainly by means of a noose structure or intron complementary pairing. Exosomes are tiny discoid vesicles with a diameter of 40-100 nm that are secreted by cells under physiological and pathological conditions. Exosomes play an important role in cell-cell communication by carrying DNA, microRNAs, mRNAs, proteins and circRNAs. In this review, we summarize the biological functions of circRNAs and exosomes, and further reveal the potential roles of exosomal circRNAs in different diseases, providing a scientific basis for the diagnosis, treatment, and prognosis of a wide variety of diseases.
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- 2022
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23. Human-Interaction Weakly-Supervised Deep Networks for Semantic Segmentation.
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Wenfeng Luo and Meng Yang 0001
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- 2019
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24. Weakly-Supervised Semantic Segmentation with Mean Teacher Learning.
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Li Tan, Wenfeng Luo, and Meng Yang 0001
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- 2019
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25. Design of the Refractive Index Sensor Based on EIT-Like Phenomenon.
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Xilong, Li, Xiaoxia, Wu, Xiaoxia, Zhao, Wenfeng, Luo, Junfang, He, and Senlin, Yang
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METAMATERIALS ,REFRACTIVE index ,DETECTORS - Abstract
In this paper, a terahertz metamaterial refractive index sensor based on electromagnetically induced transparency effect is proposed. The unit structure is composed of a closed square ring and a split circular ring. The transmission spectrum of the sensor generates sharp transparent peak at around 0.615 THz. Geometric factors on transmittance were simulated and analyzed, including the size of the opening ring, the distance between the two rings, the circular ring radius, and the refractive index of the tested object. Subsequently, the sensing performance of the device was also studied with a refractive index sensitivity of 3.68 µm/RIU. [ABSTRACT FROM AUTHOR]
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- 2024
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26. Fast Skin Lesion Segmentation via Fully Convolutional Network with Residual Architecture and CRF.
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Wenfeng Luo and Meng Yang 0001
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- 2018
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27. Human mesenchymal stem cells promote ischemic repairment and angiogenesis of diabetic foot through exosome miRNA-21-5p
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Chen Huang, Wenfeng Luo, Qian Wang, Yufeng Ye, Jinghui Fan, Li Lin, Congying Shi, Wei Wei, Hanwei Chen, Yangzhe Wu, and Yukuan Tang
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Diabetic foot ,Exosomes ,Mesenchymal stem cells ,miRNA ,Angiogenesis ,Biology (General) ,QH301-705.5 - Abstract
Background: Diabetic foot is caused by ischemic disease of lower extremities of diabetic patients, and the effective therapy is very limited. Mesenchymal stem cells (MSCs) based cell therapy had been developed into a new treatment strategy for diabetic foot clinically. However, the underlying molecular mechanism remains to be fully addressed. Exosomes (extracellular vesicles) secreted by MSCs may play crucial role in the processes of MSCs mediated inhibition of inflammatory microenvironment as well as pro-angiogenesis of ischemic tissue of diabetic foot. Methods: Exosomes were isolated from MSCs using ultracentrifugation, and further characterized by the nanoparticle tracking analyzer and flow cytometry. Moreover, RNA sequencing, Western Blot, in vitro cell proliferation, in vivo pro-angiogenesis, as well as ischemic repairment of diabetic foot through rat model were performed to evaluate exosome physiological functions. Results: We found that inflammatory cytokines (tumor necrosis factor α and interleukin-6) and vascular cell adhesion molecule-1 induced MSCs to secrete exosomes heterogeneously, including exosome size and quantity. Through RNA sequencing, we defined a new proangiogenic miRNA, miRNA-21-5p. Further knockdown and overexpression of miRNA-21-5p by manipulating MSCs validated the biological activity of exosome miRNA-21-5p, including in vitro cell proliferation, in vivo pro-angiogenesis in Chick Chorioallantoic Membrane (CAM) assay, and in vivo pro-angiogenesis experiments (tissue injury and repair) in diabetic rat models. Furthermore, we discovered that exosomemiRNA-21-5p promoted angiogenesis through upregulations of vascular endothelial growth factor receptor (VEGFR) as well as activations of serine/threonine kinase (AKT) and mitogen-activated protein kinase (MAPK). Together, our work suggested miRNA-21-5p could be a novel mechanism by which exosomes promote ischemic tissue repair and angiogenesis. Meanwhile, miRNA-21-5p could be potentially developed into a new biomarker for exosomes of MSCs to treat diabetic foot. Conclusions: miRNA-21-5p is a new biomarker and a novel mechanism by which exosomes promote ischemic tissue repair and angiogenesis of diabetic foot. Our work could not only provide new scientific evidences for revealing pro-angiogenesis mechanism of MSCs, but also eventually benefit MSCs-based clinical therapy for diabetic foot of diabetes patients.
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- 2021
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28. Comparison Between the Diagnostic Performance of 1.5 T and 3.0 T field Strengths for Detecting Deep Vein Thrombosis Using Magnetic Resonance Black-Blood Thrombus Imaging
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Yufeng Ye MS, Xueping He MS, Chen Huang MS, Caiyun Shi MS, Wei Deng MS, Wenfeng Luo MS, Jianke Liang BS, Zhuonan He BS, Huan Mao BS, Qiwei Liang MS, Dongya Chen BS, Hanwei Chen MD, and Guoxi Xie PhD
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Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background: Magnetic resonance (MR) black-blood thrombus imaging (BTI) is an accurate diagnostic technique for detecting deep vein thrombosis (DVT) but to date there have been no studies comparing the diagnostic performance and consistency of this technique at different field strengths. In this study, we evaluated and compared the diagnostic performance of BTI for detecting DVT at 1.5 T and 3.0 T field strengths. Methods: A total of 40 patients with DVT were enrolled in this study from November 2015 up to October 2018. All patients underwent BTI, a contrast-free T1-weighted MR imaging technique for detecting DVT, and contrast-enhanced MR venography (CE-MRV) at 1.5 T or 3.0 T field strengths. The MR data analyses used 1160 segments from the venous lumen of the 40 patients. The signal-to-noise ratio and contrast-to-noise ratio between thrombus and muscle/lumen were calculated to compare BTI at 1.5 T or 3.0 T to determine the image performance for thrombus detection at 1.5 T or 3.0 T. Two physicians blinded to the study evaluated all BTI images and calculated the overall sensitivity (SE), specificity (SP), positive predictive value (PPV) and negative predictive value (NPV), accuracy, and diagnostic consistency at 1.5 T and 3.0 T. These images and values were compared to control CE-MRV images that had been obtained by 2 senior physicians and used as reference standards. In addition, the reliability and consistency of diagnoses between observers were also evaluated. Results: Two study-blind physicians reviewed all BTI images to diagnose thrombus and to determine SE, SP, PPV, NPV, and accuracy. There were no statistical differences in SE, SP, PPV, NPV, or accuracy between the 1.5 T and 3.0 T groups. Conclusions: Black-blood thrombus imaging has high SE, SP, and accuracy for DVT diagnosis both at 1.5 T and 3.0 T field strengths. This noninvasive diagnostic technique, which does not require the use of contrast agents, can be widely used in the clinical screening of DVT and follow-up after treatment.
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- 2020
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29. Influence of Substrate Temperature on Electrical and Optical Properties of Sputtered Binary SnO2-Al2O3 Thin Films on Slide Glasses
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Yuanyuan, Li, Wenfeng, Luo, Shuli, Li, Zhen, Cheng, Xiaoxia, Zhao, Junfang, He, Pengfei, Zhang, and Kewei, Xu
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- 2017
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30. Plasmonic RI Sensor Design Based on Fano Resonance with MIM Waveguide Coupled by the Concentric Annulus Cavity.
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Xilong, Li, Zhaoxia, Wu, Xiaoxia, Zhao, Wenfeng, Luo, Junfang, He, Senlin, Yang, and Yuanyuan, Li
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FANO resonance ,FINITE element method ,REFRACTIVE index ,OPTICAL devices ,LIGHT transmission ,BAFFLES (Mechanical device) - Abstract
A nano resonator is designed consisting of the metal-insulator-metal (MIM) waveguide coupled with the concentric annulus cavity. The optical transmission characteristics and sensing characteristics of the designed sensor are studied by using finite element method. Experimental results show that the combination of the MIM waveguide and the concentric annulus cavity can produce bright mode and dark mode around the central wavelength of 565 nm. Due to the interaction between these modes, the Fano resonance phenomenon will be produced with a transitional dip around the wavelength of 585 nm. Compared with the impacts of the width of the metal baffle and the coupling gap, the Fano resonance effect is very sensitive to the variation of the radii of the concentric annulus cavity, which is helpful to design the tunable optical devices based on Fano resonance in the future. However the modulation depth is obviously influenced by the width of the metal baffle and the coupling gap. The research also demonstrates the sharp asymmetric Fano transmission spectrum is also sensitive to the surrounding medium. By calculating the shift of the resonant wavelength with the change of the refractive index of the medium in the resonator, the device can achieve the sensitivity of 550 nm/RIU and the figure of merit of 70. [ABSTRACT FROM AUTHOR]
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- 2024
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31. An Efficient Schema Matching Approach Using Previous Mapping Result Set.
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Hongjie Fan, Junfei Liu, Wenfeng Luo, and Kejun Deng
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- 2016
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32. Identification of joint equivalent parameters via combining PSO with FE model updating.
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Wenfeng Luo and Ling Yu
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- 2016
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33. Weakly-supervised semantic segmentation with saliency and incremental supervision updating.
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Wenfeng Luo, Meng Yang 0001, and Weishi Zheng 0001
- Published
- 2021
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34. Integration of molecular docking, molecular dynamics and network pharmacology to explore the multi‐target pharmacology of fenugreek against diabetes
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Wenfeng Luo, Jie Deng, Jiecheng He, Liang Yin, Rong You, Lingkun Zhang, Jian Shen, Zeping Han, Fangmei Xie, Jinhua He, and Yanqing Guan
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Molecular Medicine ,Cell Biology - Published
- 2023
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35. Image-guided Thermal Ablation as a Promising Approach to Both Nontoxic and Toxic Autonomously Functioning Thyroid Nodules
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Long Wang, Peiqing Wang, Zhijiang Chen, Yinghe Lin, Yingshan Liu, Rong Peng, Jinlian Li, Wenfeng Luo, and Jian Kuang
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Radiology, Nuclear Medicine and imaging - Published
- 2023
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36. Design and research of dual-wavelength polarization multiplexing multifocal metalens based on superimposed nano-antenna array
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Shuyuan Lv, Jie Jia, Wenfeng Luo, and Xinhui Li
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metalens ,polarization multiplexing ,dual-wavelength ,the superimposed structure ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Chemical technology ,TP1-1185 - Abstract
In this study, based on the single-layer metasurface structure, a dual-wavelength polarization multiplexing metalens is designed at the communication wavelengths of 1310 nm and 1550 nm, respectively. Using the dual-phase modulation method, a single-wavelength polarization multiplexing metalens is proposed, which can simultaneously control left-handed circularly polarized light (LCP) and right-handed circularly polarized light (RCP). Furthermore, the cross superposition method is used to combine them to achieve the dual-wavelength polarization multiplexing metalens. The results show that the system can achieve polarization multiplexing at the two wavelengths of 1310 nm and 1550 nm, which is consistent with the expected results, and the focusing efficiency reached 61%. With the increase of the numerical aperture, the focusing intensity of the left and right focal points gradually approaches, and the difference between the full width at half maximum of the two focal points also decreases accordingly. It provides a new way for the optical imaging, information detection and the realization of multifunctional ultra-surface devices.
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- 2021
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37. Numerical study of an electrowetting liquid microlens
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Shuyuan Lv, Chongqi Liu, and WenFeng Luo
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Physics ,QC1-999 - Abstract
We construct a numerical model for a liquid microlens formed by filling a microwell with two immiscible liquids, namely oil and water. The water–oil interface can be actuated as a tunable liquid lens because its curvature is well controlled by applying an external electric field. The contact angle of the oil droplet has the opposite tendency to that of a traditional water-filled microwell because of the repulsion force from the water phase. We study the dynamic interface deformation as functions of time and applied voltage, which typically within 10 ms is good enough as an imaging system for human eyes. In addition, using two different filling liquids provides more possibilities for tuning the focal length.
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- 2018
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38. Design of dual-wavelength polarization control metasurface lens
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Wenfeng LUO, Xinhui LI, Shuyuan LYU, and Jie JIA
- Subjects
General Engineering - Abstract
With the rapid development of modern optics, optical elements have become an indispensable part of an optical system. A metasurface is a nanostructure composed of arrays of sub-wavelength scatterers and is widely used due to its simple structure, thin thickness, easy integration, and high utilization rate. This paper designs a polarization-multiplexed transmissive metasurface lens in the visible light band 690 nm and near-infrared light band 880 nm. The metasurface lens combines the x-polarized lens design with the y-polarized lens design to realize three metasurface lenses with dual wavelength and different polarization states under the same metasurface. The metasurface lenses are: a coaxial confocal metasurface lens with the focus length of f1=f2=7 215 nm, an off-axis metasurface lens with the focus length of f1=f2=7 221 nm and with a displacement of xd=±4 000 nm, and a coaxial metasurface lens with the focus length of f1=7 000 nm and f2=10 000 nm, respectively. They have not only a high numerical aperture of 0.8 but also a good focusing capability with a full width at half maximum close to diffraction limit, and their space utilization is also improved. This compact and highly numerical aperture and high spatial utilization of dual-wavelength polarization multiplexing metasurface design provides an effective solution for the development of focusing lens and has unique potentials and advantages in fluorescent microlens, optical imaging, etc.
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- 2022
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39. Harmonic Mode-Locked Er-Doped Fiber Laser Based a Microfiber-Based Pbs Nanoparticle Saturable Absorber
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Liang Nie, Lu Wang, Xiaoyu Liu, Wenfeng Luo, Chong Wang, Wei Wang, Jiangjiang Feng, Ge Gao, xiaohui li, and Kai Zhang
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- 2023
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40. Design of off-axis and co-axial achromatic bifocal metalens
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Shuyuan Lv, Fei Meng, WENFENG LUO, Yuchi Bai, and Rong Wang
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- 2022
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41. The Application of Taboo Search with Kick Strategy in Surface Mounting Arrangement.
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Wenfeng Luo, Jiaxiang Luo, Zijian Mo, Runpeng Liang, and Qingqiang Yang
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- 2008
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42. PSO Combined with ILS for Flowshop-Based Parallel Task Assignment Problem.
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Runpeng Liang, Jiaxiang Luo, Qingqiang Yang, and Wenfeng Luo
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- 2008
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43. Study on double-layer adjustable metalens based on phase change material Sb2S3
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Shuyuan Lv, Jie Jia, Wenfeng Luo, and Xinhui Li
- Subjects
General Engineering ,Atomic and Molecular Physics, and Optics - Published
- 2022
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44. Construction of a double-responsive modified guar gum nanoparticles and its application in oral insulin administration
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Mingze Xu, Han Qin, Yuxin Zheng, Jiapeng Chen, Xuanxi Liang, Jinpeng Huang, Wenfeng Luo, Runcai Yang, and Yan-Qing Guan
- Subjects
Drug Carriers ,Colloid and Surface Chemistry ,Glucose ,Drug Delivery Systems ,Plant Gums ,Insulin ,Nanoparticles ,Administration, Oral ,Surfaces and Interfaces ,General Medicine ,Physical and Theoretical Chemistry ,Hydrogen-Ion Concentration ,Biotechnology - Abstract
The use of intelligent insulin delivery systems has become more important for treating diabetes. In this study, a dual-responsive oral insulin delivery nanocarrier that responds to glucose and pH has been developed. First, the oleic acid hydrophobic modified guar gum (GG) was synthesized by the esterification reaction, and the γ-polyglutamic acid (γ-PGA) was coupled with GG by the amidation reaction. The obtained pH-responsive copolymer (γ-PGA-GG) was cross-linked by concanavalin A to obtain pH/glucose dual-responsive nanocarriers, and insulin was effectively loaded into the dual-responsive nanocarriers. The insulin-loaded nanoparticles can achieve effective pH and glucose responses, releasing insulin on demand. In vitro and in vivo studies demonstrated the dual-responsive nanoparticles can protect insulin against the pH changes in the digestive tract and deliver insulin into the body to exert a hypoglycemic effect. Moreover, the dual-responsive nanoparticles have significant potential to be employed for oral insulin delivery.
- Published
- 2022
45. A portable smartphone-based hemoglobin point-of-care testing platform for accurate anemia diagnostics
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Qiangqiang Fu, Tao Qi, Ze Wu, Yongjian He, Shujuan Guan, Shihua Luo, Qi Zhang, Wenfeng Luo, Wei Xiao, Bo Situ, and Lei Zheng
- Subjects
Point-of-Care Systems ,Biomedical Engineering ,Biophysics ,Anemia ,General Medicine ,Biosensing Techniques ,Hemoglobins ,Point-of-Care Testing ,Pregnancy ,Electrochemistry ,Humans ,Female ,Smartphone ,Child ,Biotechnology - Abstract
Anemia affects over 2 billion people worldwide, with the heaviest burden borne by women and children. At present, anemia is diagnosed by measuring hemoglobin (Hb) levels, which must be done in hospitals or commercial laboratories by skilled operators. In this work, we report a portable, affordable ($3), easy-to-operate (1 min) and accurate smartphone-based Hb analyzer (SHbA) that uses a drop of finger-pricked blood for anemia point-of-care test (POCT) applications. POCT of Hb was achieved using a smartphone ambient light sensor (ALS) to accurately measure the absorbance of colorimetric Hb biochemical analysis reagents in a microcuvette, as well as an Android-based application for results analysis. SHbA validation results agreed well with those reported by a hematology analyzer, and the SHbA has an anemia diagnosis sensitivity of 95.4% and specificity of 96.3% for venous blood (n = 360) and a sensitivity of 96.39% and specificity of 95.58% for fingertip blood (n = 475). In addition, SHbA exhibits excellent performance in the diagnosis and treatment guidance of anemia high-risk populations, including tumor chemotherapy patients (n = 424), pregnant women (n = 214) and thalassemia patients (n = 208). Importantly, volunteer self-testing results (n = 20) indicate that SHbA can be used for home-based anemia diagnosis and monitoring. SHbA has the advantages of high sensitivity and specificity while being cheap and easy to operate, making it widely applicable for the diagnosis and treatment of anemia, especially for high-risk patients in areas with poor medical resources.
- Published
- 2022
46. The RNA methylase FTO mediates circRNF220 demethylation, and circRNF220 binds to serine/arginine-rich splicing factor 1 to promote the progression of acute myeloid leukemia
- Author
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JinHua He, ZePing Han, WenFeng Luo, Jian Sen, FangMei Xie, Ting Pan, Qu Hong, ZhongHui Guo, JianHao Li, YuGuang Li, and HanWei Cheng
- Abstract
Background: The key circular RNAs (circRNAs) and the molecular mechanisms by which they regulate the progression of acute myeloid leukemia (AML) in adults are unclear.Methods: We used a circRNA microarray to analyze the differentially expressed circRNAs in peripheral blood mononuclear cells of newly diagnosed adult AML patients. Reverse transcription-PCR, fluorescence in situ hybridization, Sanger sequencing, and ribonuclease R tests were used to examine circRNF220 loop formation and stability and its potential value in AML diagnosis. Cell Counting Kit-8, flow cytometry, and subcutaneous tumor assays were used to detect the effects of circRNF220 on the proliferation and apoptosis of the HL-60 and THP-1 cell lines. RNA antisense purification, methylated RNA immunoprecipitation, and RNA pull-down assays were used to identify circRNF220-binding proteins and microRNAs.Results: CircRNF220 was located in the cytoplasm and was more stable than linear RNF220. The area under the curve value of circRNF220 for the diagnosis of AML was 0.8314. Silencing circRNF220 expression inhibited the growth and promoted the early apoptosis of HL-60 and THP-1 cells. CircRNF220 had no competitive endogenous relationship with microRNAs. The RNA methylase FTO regulated the demethylation and expression of circRNF220. The RNA-binding protein serine/arginine-rich splicing factor 1 (SRSF1) and circRNF220 co-localized in the cytoplasm, and circRNF220 promoted SRSF1 expression. Furthermore, silencing circRNA220 reduced the tumorigenicity of AML cells in vivo.Conclusions: CircRNF220 is a potential biomarker for the diagnosis of adult AML. FTO mediates circRNF220 demethylation. and circRNF220 binds to SRSF1 to promote the progression of AML.
- Published
- 2022
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47. Progress in research on tumor microenvironment-based spatial omics technologies.
- Author
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FANGMEI XIE, NAITE XI, ZEPING HAN, WENFENG LUO, JIAN SHEN, JINGGENG LUO, XINGKUI TANG, TING PANG, YUBING LV, JIABING LIANG, LIYIN LIAO, HAOYU ZHANG, YONG JIANG, YUGUANG LI, and JINHUA HE
- Subjects
TUMOR microenvironment ,EXTRACELLULAR matrix ,BLOOD vessels ,PROTEOMICS ,RESEARCH methodology - Abstract
Spatial omics technology integrates the concept of space into omics research and retains the spatial information of tissues or organs while obtaining molecular information. It is characterized by the ability to visualize changes in molecular information and yields intuitive and vivid visual results. Spatial omics technologies include spatial transcriptomics, spatial proteomics, spatial metabolomics, and other technologies, the most widely used of which are spatial transcriptomics and spatial proteomics. The tumor microenvironment refers to the surrounding microenvironment in which tumor cells exist, including the surrounding blood vessels, immune cells, fibroblasts, bone marrow-derived inflammatory cells, various signaling molecules, and extracellular matrix. A key issue in modern tumor biology is the application of spatial omics to the study of the tumor microenvironment, which can reveal problems that conventional research techniques cannot, potentially leading to the development of novel therapeutic agents for cancer. This paper summarizes the progress of research on spatial transcriptomics and spatial proteomics technologies for characterizing the tumor immune microenvironment. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
48. High-Repetition-Rate-Modulated Pulsed Fiber Laser Based on FeS2
- Author
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Xiaohui Li, Yujie Ren, Xiaoyu Liu, Wenfeng Luo, Tikang Shu, Hongmei Chen, Yang Zhao, Ziyang Zhang, Hongpei Wang, Shuyuan Lv, and Xu Wang
- Subjects
Materials science ,Repetition (rhetorical device) ,Field (physics) ,business.industry ,Band gap ,Physics::Optics ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Light absorption coefficient ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Ferrous disulfide ,General Energy ,Fiber laser ,Optoelectronics ,Physical and Theoretical Chemistry ,0210 nano-technology ,business ,Ultrashort pulse - Abstract
Pyrite-type ferrous disulfide (FeS2) is very suitable for ultrafast optical applications in the near-infrared field due to its appropriate band gap and high light absorption coefficient. However, n...
- Published
- 2021
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49. CoS2 with 3D Valence Electron Shell Structure for Pulsed Laser Application
- Author
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Hongmei Chen, Yujie Ren, Chong Wang, Wenfeng Luo, Lirong Jing, Ziyan Wu, Mengjia Qu, Ziyang Zhang, Xiaohui Li, and Xu Wang
- Subjects
Pulsed laser ,Nonlinear optical ,Materials science ,Mode-locking ,business.industry ,Physics::Optics ,Optoelectronics ,General Materials Science ,Porosity ,Valence electron ,business ,Ultrashort pulse ,Nanomaterials - Abstract
CoS2 with a three-dimensional porous structure has attracted great interest because of its excellent electrical, magnetic, and optical properties. However, the nonlinear optical properties of CoS2 ...
- Published
- 2020
- Full Text
- View/download PDF
50. Electrochemical Peeling Few-Layer SnSe2 for High-Performance Ultrafast Photonics
- Author
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Shuyuan Lv, Zhaojiang Shi, Xiaohui Li, Wenxiong Xu, Wenfeng Luo, Chenxi Zhang, Yujie Ren, Xiaoyu Liu, and Kai Zhang
- Subjects
Electron mobility ,Materials science ,Band gap ,business.industry ,Physics::Optics ,Saturable absorption ,Laser ,Q-switching ,law.invention ,law ,Fiber laser ,Optoelectronics ,General Materials Science ,Photonics ,business ,Absorption (electromagnetic radiation) - Abstract
In recent years, the photoelectric properties and nonlinear optical properties of layered metal chalcogenides (LMCs) have attracted extensive attentions. Because of lower phonon thermal conductivity, larger energy storage rate, and larger electron mobility, LMCs are widely studied in the fields of thermoelectric energy conversion, battery electrode materials, and semiconductor devices. As 2D LMCs, SnSe2 nanosheets (Ns) are connected to each other by van der Waals force, which makes it possible to use electrochemical methods to help peel off the thin layer structure. Two-dimensional SnSe2 has obvious adjustable band gap characteristics. Its thickness can be controlled to keep it on the desired band gap. In this article, we prepared a thin layer of SnSe2 by electrochemical methods and detected its nonlinear optical characteristics. It shows that our prepared materials have good optical absorption characteristics; it has a modulation depth of 15% and a saturation intensity of 61 MW/cm2. To investigate the nonlinear effects of SnSe2 in short and long cavities, the Q-mode-locking phenomenon was first achieved in a fiber laser with cavity length of 6 m. After increasing the cavity length to 56 m, the pump power is adjusted to achieve an adjustable repetition frequency from MHz to GHz in turn in an Er-doped fiber laser through utilizing an SnSe2 incorporating a tapered fiber as a saturable absorber (SA). The nonlinear optical properties of thin layer SnSe2 are fully proven, which opens a new way for advanced photonics, optical communication, laser measurement, and other fields.
- Published
- 2020
- Full Text
- View/download PDF
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