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Healthcare facilities produce millions of tons of waste annually, with a significant portion consisting of diagnostic plasticware. Here, we introduce a new detection platform that completely replaces traditional assay plates with a piece of membrane, offering a much greener and more sustainable alternative. The membrane, integrated within the portable vortex fluidic device (P-VFD), enables rapid detection of a clinically relevant protein biomarker, urinary p75ECD. This biomarker is utilized to evaluate the prognosis, disease severity, and progression of amyotrophic lateral sclerosis (ALS). This assay has a limit-of-detection (LOD) of 4.03 pg, which is comparable to the plate-based assay (2.24 pg) and has been optimised through a full factorial design of experiments (DOE) and response surface methodology (RSM). P-VFD has great potential in quantifying p75ECD in human biofluids and can significantly reduce the assay time to 5 min compared to the current plate-based p75ECD ELISA assay (3 days), with at least a 4.4-fold reduction in the usage of the detection antibody.

This article details how to use a vortex fluidic device (VFD) to accelerate protein purification via immobilized metal affinity chromatography (IMAC). Building upon a previous report of VFD-based purification, we introduce a membrane insert to simplify the purification protocol and the resin recovery step. This new platform can be adapted to different types of IMAC resins and purification membranes. Proteins can be purified directly from clarified lysate, non-clarified lysate, and even non-lysed cultures without concerns of system clogging. Strong binding between the Ni2+ and the target protein's His6-tag effectively captures the target protein on IMAC resins or membranes placed in the VFD. Continuous flow of different solutions through the VFD allows dynamic binding, washing, and elution of the target protein. Furthermore, the system dramatically accelerates protein purification; a typical purification from cell lysate requires approximately 4 min. Herein, we demonstrate the single-step purification of two His6-tagged proteins from both clarified and non-clarified cell lysates without requiring batch binding. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Preparation of the resin-loaded membrane insert and the vortex fluidic device (VFD) setup prior to purification Basic Protocol 2: Purification of His6-tagged proteins using the VFD Alternate Protocol: VFD-mediated His6-tagged protein purification from non-clarified lysate Support Protocol: Preparation of chemically modified glass fiber membrane for VFD-mediated immobilized metal affinity chromatography purification.

The article discusses the need for the new Labour government to address the broken immigration and asylum systems in the UK. It emphasizes the importance of establishing a humane asylum system, implementing a workers' rights-based approach to immigration, and developing a program for integration, stability, and citizenship. The article also highlights the need for a positive narrative on population and immigration to counter the toxic conversation surrounding immigration in the UK. It calls for policy changes that prioritize fairness, equality, and the well-being of migrants and refugees. [Extracted from the article]

As UK cities and towns become increasingly ethnically and culturally diverse, researchers have tuned into how people inhabit multiculturalism. Ethnographic approaches have focused on the kind of togetherness that people generate as they go about their everyday lives, observing the affective textures of interactions and happenings of the here and now in granular detail. Missing from these accounts is what crowdsourced data might add to understandings of how multicultural places are experienced. What is vital about this kind of data is that it is 'big', involving a diversity of voices similarly intent on messaging their experiences, presenting opportunities to scale up the affect of encounters and to quantify what is difficult to qualify. This paper brings Digital Geographies into conversation with research on everyday multiculturalism to examine qualitatively and quantitatively how social media use folds into and expresses various practices of sociality and connection. Our paper involves Twitter, Nando's and the city of Leicester in the UK to challenge and advance ways of understanding everyday multiculturalism in an era of global migration and ethnically complex populations. [ABSTRACT FROM AUTHOR]

The article in ChemSusChem discusses a new method for detecting the protein biomarker p75ECD, which is relevant for diagnosing Amyotrophic Lateral Sclerosis (ALS). The researchers have developed a portable vortex fluidic device (P‐VFD) with a membrane array to enable fast and sustainable biomarker measurement. This innovation aims to reduce plastic waste generated by traditional assay plates, offering a greener alternative for biofluid analysis. The study was conducted by X. Luo, M.‐L. Rogers, C. L. Raston, and their colleagues. [Extracted from the article]

Background: Various natural products are reported to improve maternal milk supply yet are not necessarily safe for infants. Researchers have not systematically studied galactagogue teas for safety. Research aim: This study evaluates the safety of a galactagogue tea in breastfeeding women and their infants, assessing short- and long-term adverse effects. Methods: Healthy, exclusively/fully breastfeeding women (N = 60) with no milk insufficiency were randomized into (1) an all-natural tea containing fruits of bitter fennel, anise, and coriander; fenugreek seed; and other herbs (Mother's Milk® herbal tea; test) group or (2) a lemon verbena leaf (placebo) group. Maternal diaries captured self-reported maternal and infant adverse effects, tea consumed, and perceived infant satisfaction. Maternal quality of life and psychological state were assessed at baseline and 2 and 4 weeks. Poststudy calls assessed adverse effects through the infants' age of 12 months. Results: No adverse effects attributable to the interventions were reported at any time point. No differences were found between test and placebo groups in sociodemographic characteristics, maternal or infant adverse symptoms, quality of life, breastfeeding self-efficacy, maternal psychological measures, infant growth, and infant satisfaction (all p >.05). Conclusions: This double-blind, randomized controlled trial (RCT) of an herbal galactagogue versus placebo among healthy, exclusively/fully breastfeeding mothers and infants found no adverse events associated with the test tea across the 30-day study or the first year of their infant's life. This composite tea appears to present no safety risk for mothers or their young babies. [ABSTRACT FROM AUTHOR]

Current approaches to the discovery of scientific resources (publications, data sets and web services) are dominated by keyword search. These approaches do not allow scientists to search on the deeper semantics of scientific resources, or to discover resources on the basis of the scientific approaches taken. This article evaluates a user interface that allows users to discover scientific resources through structured knowledge in the form of ontologies describing the domain and the scientific knowledge inherent within the scientific resource, and also through informal user tags. These combined capabilities provide scientists with new and powerful options for resource discovery. A qualitative user evaluation explored how scientists felt about the approach for resource discovery in the context of their scientific work. The study showed that marine scientists were enthusiastic about the capabilities of such an approach and appreciated the ability to browse the visual structure of the knowledge and query on scientific method but, overall, preferred the use of tags over ontologies. The exploratory nature of the user study was used to identify future directions for such improvements. [ABSTRACT FROM AUTHOR]

Presents a horticultural perspective of herb and medicinal plant production in the U.S. from 1903 to 2003. Overview of the market; Information on mint and ginseng.