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Copyright of Indonesian Journal of Veterinary Science / Jurnal Kedokteran Hewan is the property of Universitas Syiah Kuala, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Efforts to find alternative medicines or herbal-based interventions for diabetes sufferers are very necessary. The aimed of this study was to find out whether the use of okra fruit infusion (Abelmoschus esculentus) with different administration frequencies can reduce the blood sugar levels of hyperglycemic white rats (Rattus norvegicus), and to find out what frequency of giving okra fruit infusion can reduce blood sugar levels. white rat (Rattus norvegicus) tallest. This type of research is true experimental, consisting of 5 treatment groups, (P0-, P0+, P1, P2, and P3), namely negative control, positive control, giving 6 ml of okra fruit infusion water/day given once, giving infused water 6 ml of okra fruit/day was given 2 times a day and 6 ml of okra fruit infusion water was given 3 times a day. Each group consists of 5 repetitions. The research was carried out at the Chemistry Laboratory, Muhammadiyah University of Malang. The data collection method was by measuring the blood glucose levels of white mice that had been treated, then analyzing them using SPSS. Based on the results of data analysis and discussion, it can be concluded that there is an effect of the frequency of administration of okra fruit infusion on reducing blood sugar levels in white rats (hyperglycemia. Treatment P3 is a group of hyperglycemic rats that were given okra fruit infusion water at a dose of 6 ml/head/day, given once a day in the morning and P2, namely the group of hyperglycemic mice that were given okra fruit-infused water at a dose of 6 ml/head/day, given 2x a day in the morning and evening, is the best treatment because it has the same notation as P0 (negative control) or the group of mice that not given water infused with okra fruit and not given alloxan. Giving an infusion of okra fruit at a dose of 6 ml/head given once in the morning or twice (morning and evening) has an effect on reducing the blood sugar levels of hyperglycemic white mice. Okra can be consumed fresh, processed by boiling, or frying, dry, but not made into flour.

The purpose of the research is a comparative study of morphological changes in Trichinella spiralis capsules and larvae in the lynx and laboratory animals.Materials and methods. The experiment used striated muscle tissue from spontaneously infected lynxes and from white rats experimentally infected with T. spiralis larvae isolated from wild animals in the Kirov Region. Morphometric studies of T. spiralis capsules and larvae in the lynx muscle tissue were conducted on temporary histologic specimens.Results and discussion. Lemon-shaped (50%) and oval (40%) capsules were found in the entire muscle mass of the white rats, and only 10% of the larvae were round in shape. The lynx was not found to have lemon-shaped capsules; oval (60%) and round (40%) capsules predominated. The studies show that the parasite capsule size and shape indicate the adaptive abilities of T. spiralis to various types of host muscle tissue and their morphological characteristics, and are largely determined by the symplast structure and size.

Aim. To study the morphology of neurons in the cerebral cortex of rat pups on day 20 under conditions of administration of a nitric oxide synthase inhibitor (NOS) during placentation.Materials and methods. Outbred white female rats (n = 12) were randomly divided into 2 groups of 6 rats each. On the 11th day of pregnancy, the experimental group received a single intramuscular injection of N(omega)-nitro-L-arginine methyl ester (L NAME) at a dose of 25 mg/kg, in the control group – once intramuscularly 0.9% NaCl solution. Born rat pups were randomly selected one from the mother. On the 20th day, after medical euthanasia, the brain was collected. In the anterior part of the frontal cortex, we studied the density and area of neurons, the size and shape of perikarya and the severity of their staining with toluidine blue. Results. In the experimental group of 20-day-old rat pups, compared to the control group, the density and area of neurons were less by 10% (p > 0.05) and 22% (p > 0.05), respectively, the shape of the perikarya also changed to elongated, the elongation factor increased by 0.3 units. (p < 0.05) and there was a sixfold increase in the proportion of hyperchromic neurons (p < 0.05), hyperchromic wrinkled (p < 0.001) neurons appeared, which were absent in control animals. Conclusion. Morphological changes in neurons of the cerebral cortex in rat pups born from females who received a NOS inhibitor during placentation may be a consequence of a decrease in the formation of nitric oxide in the neurons themselves and in the endothelium of the vessels supplying the brain

Copyright of Acta Vet Indones. The Indonesian Veterinary Journal / Jurnal Acta Veterinaria Indonesiana is the property of IPB University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Indonesian Journal of Veterinary Science / Jurnal Kedokteran Hewan is the property of Universitas Syiah Kuala, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In our research, we were interested in the actual presence of adaptive or negative reactions in the wall of the small intestine of white rats under the influence of gadolinium orthovanadate nanoparticles in the range of doses (≈0.03-0.3 mg/kg of body weight) under conditions of food stress (due to an excess of fiber and lack of protein in the diet) and their degree of manifestation, since this type of ration disproportion occurs quite often in Ukraine. Nanoparticles of gadolinium orthovanadate have a significant potential for use in animal husbandry and poultry farming, as in the range of doses of 0.03-0.15 mg/kg of body weight, they prevent negative effects on the intestinal mucosa, even in conditions of feed stress. It has been established that administration of gadolinium orthovanadate nanoparticles in doses of 0.03 and 0.15 mg/kg of body weight to white rats with drinking water for 56 and 28 days, respectively, leads to activation of the mechanical and immunological barrier of the mucous membrane, as indicated by an increase goblet cells, hyperplasia of enterocytes of some crypts, thickening of villi and infiltration by lymphocytes of the own plate, which reach the control level 14 days after stopping their administration. However, increasing the dose of gadolinium orthovanadate nanoparticles to 0.3 mg/kg of body weight in conditions of food stress leads to the depletion of the adaptive capabilities of the intestinal mucosa and excessive activation of the immunological barrier, which were manifested by dystrophic changes from the 14th day of administration, which deepened to the 56th day and do not level off after 14 days after stopping administration. [ABSTRACT FROM AUTHOR]

The prohibition of using antibiotics with growth-stimulating properties in the European Union led to the search for new, safer, more effective, and cheaper feed additives. One of these substances was rare earth elements (REE, atomic numbers 57–71) due to their low toxicity and protective and antioxidant properties. At the same time, higher efficiency of organic forms of REE was noted. Thanks to this, it is possible to assume their even more pronounced effect in nano-sized form as feed additives and the need to conduct research in this direction. Therefore, this work aims to study the metabolic parameters of the blood in rats under long-term oral administration of nanoparticles of one of the representatives of rare earth elements – gadolinium orthovanadate (NP GdVO4:Eu3+) under food stress. Experimental samples of gadolinium orthovanadate nanoparticles activated by Europium (spindle-shaped geometry; size 8×25 nm; initial concentration 1.0 g/dm³ ) were used in work. Experimental studies on rats were carried out based on the vivarium of the NSC “IEKVM”. The object of research was 140 mature male Wistar rats with an initial weight of 180–200 g. Four groups of animals, 35 rats each, were formed according to the principle of analogs. During the experiment, animals of the control group received drinking water without additives; rats of the І experimental group were given a solution of NP GdVO4:Eu3+ at a dose of 0.2 mg/dm³ (≈ 0.03 mg/kg of body weight); II research group – at a dose of 1.0 mg/dm³ (≈ 0.15 mg/kg of body weight) and rats of III research group – at a dose of 2.0 mg/dm³ (≈ 0.30 mg/kg of body weight). Drinking was carried out for 56 days, then it was completed, and the rats were observed for another 14 days. A nutritionally unbalanced diet was used as a stress factor. Taking into account the results of biochemical studies, the adaptogenic effect of NP GdVO4:Eu3+ in the range of doses of 0.2–1.0 mg/dm³ of drinking water (≈ 0.03–0.15 mg/kg of body weight) on the body of white rats was established under conditions of food stress with optimal duration of action – 28–42 days. Under the conditions of administration at a dose of 2.0 mg/dm³ of drinking water (≈ 0.30 mg/kg of body weight), a hepatic(cyto-)toxic effect of nanoparticles was detected, which was accompanied by an irreversible decrease in the structural indicators of lipid metabolism, the consumption of antioxidant resources and the induction of intensity processes of lipid peroxidation against the background of alanine aminotransferase hyperenzymemia. [ABSTRACT FROM AUTHOR]

The study of food products is the most important method for creating proves of their food safety based on generally accepted scientific ideas and current international and national legal norms of food safety. At the stage of laboratory research it is possible to objectively prove the efficiency and safety of new types of food products while their consumption according to certain health indications among the relevant group of people, including staff working in hazardous industrial environment. In the Russian Federation the procedure for assessing the safety and running the laboratory tests of food products are regulated by the Federal Laws on the Quality and Safety of Food Products, regulations on provision of public catering services, methodological recommendations for assessing the risk to public health in case of exposure to microbial factors contained in food products, as well as technical regulations of the Customs Union on food safety. The authors have developed regulatory and technical documentation for the cereal product “Cereal bar” and presented data on its safety when consumed. Conclusions about the product are made on the basis of a study of its chronic toxicity. It has been established that the ingredients composition of the cereal bar, included in the diet of laboratory animals, provided a positive effect on their body condition, weight gain and did not cause abnormal changes in the volume of internal organs and the development of pathologies that could indicate a side effect of the product. During study of the hematological and biochemical parameters, no significant differences were found between the data of the control group and experimental group of the animals. The conducted studies of the cereal product “Cereal bar” confirm the absence of a potential risk of developing chronic toxicity in case of introduction of the cereal bar to the animals’ diets and its consumption over a long period of time.

The purpose of the research is to study the embryotropic effect of supramolecular complex of fenbendazole (SMСF).Materials and methods. The experiment to assess the embryotropic properties of SMCF was carried out on 40 white female and 20 male rats in accordance with the Guidelines for the experimental (preclinical) study of new pharmacological substances. Pregnant female rats were divided into 3 experimental and one control groups. SMCF was administered intragastrically on the 1–6 days of embryogenesis (group 1); on the 7–14 days (group 2) and on the 15–19 days (group 3) in three times therapeutic dose – 6,0 mg/kg of active substance. The animals of the control group received saline from the first to the 19th days of pregnancy. Rats were euthanized on the 20th day of pregnancy. The uterus with fetuses was removed after laparotomy, the number of corpora lutea, implantation sites, the number of living, dead and resorbed fetuses were recorded, the weight and diameter of the placenta were determined. The embryos were examined, weighed, the craniocaudal sizes were determined, the levels of total embryonic, preimplantation and postimplantation embryo death were calculated. The fetuses were examined for abnormalities of internal organs and changes in the skeletal system according to the methods of J. G. Wilson (1965) and A. B. Dawson (1926), modified in the department of embryology of the IEM of the USSR Academy of Medical Sciences.Results and discussion. As a result, SMCF does not induce toxic effects on the fetus: mortality rates, size and weight of embryos were at the level of the control group in a threefold therapeutic dose 6,0 mg/kg at intragastric administration on the 1–6; 7–14 and 15–19 days of pregnancy. SMCF did not cause external and internal malformations.

In our research, we were interested in the actual presence of adaptive or negative reactions in the wall of the small intestine of white rats under the influence of gadolinium orthovanadate nanoparticles in the range of doses (≈0.03-0.3 mg/kg of body weight) under conditions of food stress (due to an excess of fiber and lack of protein in the diet) and their degree of manifestation, since this type of ration disproportion occurs quite often in Ukraine. Nanoparticles of gadolinium orthovanadate have a significant potential for use in animal husbandry and poultry farming, as in the range of doses of 0.03-0.15 mg/kg of body weight, they prevent negative effects on the intestinal mucosa, even in conditions of feed stress. It has been established that administration of gadolinium orthovanadate nanoparticles in doses of 0.03 and 0.15 mg/kg of body weight to white rats with drinking water for 56 and 28 days, respectively, leads to activation of the mechanical and immunological barrier of the mucous membrane, as indicated by an increase goblet cells, hyperplasia of enterocytes of some crypts, thickening of villi and infiltration by lymphocytes of the own plate, which reach the control level 14 days after stopping their administration. However, increasing the dose of gadolinium orthovanadate nanoparticles to 0.3 mg/kg of body weight in conditions of food stress leads to the depletion of the adaptive capabilities of the intestinal mucosa and excessive activation of the immunological barrier, which were manifested by dystrophic changes from the 14th day of administration, which deepened to the 56th day and do not level off after 14 days after stopping administration. PATOMORFOLOŠKE SPREMEMBE V DVANAJSTNIKU PODGAN OB SUBKRONIČNEM PERORALNEM DAJANJU NANODELCEV GADOLINIJEVEGA ORTOVANADATA OB PREHRANSKEM STRESU Izvleček: V naši raziskavi nas je zanimala dejanska prisotnost prilagoditvenih ali negativnih reakcij v steni tankega črevesa belih podgan pod vplivom nanodelcev gadolinijevega ortovanadata v razponu odmerkov (≈ 0,03–0,3 mg/kg telesne teže) v pogojih prehranskega stresa (zaradi presežka vlaknin in pomanjkanja beljakovin v prehrani) in njihova stopnja izražanja, saj se tovrstna nesorazmernost obrokov v Ukrajini pogosto pojavlja. Nanodelci gadolinijevega ortovanadata imajo pomemben potencial za uporabo v živinoreji in perutninarstvu, saj v območju odmerkov 0,03–0,15 mg/kg telesne teže preprečujejo negativne učinke na črevesno sluznico tudi pri stresu zaradi krme. Ugotovljeno je bilo, da dajanje nanodelcev gadolinijevega ortovanadata v odmerkih 0,03 in 0,15 mg/kg telesne teže belim podganam s pitno vodo 56 oziroma 28 dni povzroči aktivacijo mehanske in imunološke pregrade sluznice, kar se kaže v povečanju števila čašastih celic, hiperplaziji enterocitov nekaterih kript, zadebelitvi resic in infiltraciji limfocitov, ki 14 dni po prenehanju dajanja dosežejo kontrolno raven. Vendar pa povečanje odmerka nanodelcev gadolinijevega ortovanadata na 0,3 mg/kg telesne teže pri prehranskem stresu povzroči izčrpavanje prilagoditvenih sposobnosti črevesne sluznice in pretirano aktivacijo imunološke pregrade, kar se je od 14. dneva dajanja pokazalo z distrofičnimi spremembami, ki so se poglobile do 56. dne in se po 14 dneh po prenehanju dajanja niso izravnale. Ključne besede: redke zemeljske kovine; nanodelci gadolinijevega ortovanadata; patomorfološke spremembe; dvanajstnik; bele podgane; krmni stres

Research Background : In the process of childbirth, there is often a tear in the birth canal, especially in the perineum, which is known as a ruptured perineum. Some herbal ingredients that are thought to be healing wounds are nyamplung seed oil/tamanu oil (Calophyllum inophyllum L.), red ginger oil (Zingiber officinale var rubrum) and marigold flower oil/calendula oil (Calendula officinalis). Calendula ointment has the benefit of healing episiotomy wounds faster than the use of povidone iodine. Research Objective: To determine the effectiveness of giving calendula oil herbal ingredients to wound healing in female white rats. Research Methods: Laboratory experimental research using a completely randomized design using the Post Test Only Control Group Design pattern. The population in this study were female white rats (Rattus norvegicus). The sample selection was used by simple random sampling. Univariate analysis of wound healing was performed to obtain the mean ± SD and bivariate analysis using one-way ANOVA using computer software. The results of the study: The results of statistical tests showed that the One way Anova test obtained p value = 0.000 with a value of = 0.05 (p < ) where the administration of Calendula Oil on white rat wounds was more effective than without treatment. Conclusion: Based on the results of the study, it can be concluded that giving calendula oil to female white rats is effective in healing wounds.

An experimental study is devoted to the study of the effect of Astragalus vulpinus extract on lipid peroxidation processes in the hypothalamic region under conditions of lipopolysaccharide immune stress. Material and methods. Experiments were performed on white male rats 6-8 months of age. In all series of experiments, animals were divided into groups (n = 10): 1st – control individuals receiving distilled water in an equivalent volume; 2nd – rats with immune stress model; 3rd – individuals treated with Astragalus vulpinus extract against the background of immune stress. The test extract and distilled water were administered once a day intragastrically with a 50 mg/kg probe for 14 days. Immune stress was formed by single intraperitoneal administration of lipopolysaccharide pyrogenal isolated from Salmonella typhi microbial cells. The intensity of free radical oxidation processes was studied by thiobarbituric acid reactive substances (TBARS) content, spontaneous and ascorbate-dependent lipid peroxidation rate and catalase activity were also determined. Results. According to the results of the experiment, it was revealed that immune stress is accompanied by the activation of free-radical oxidation processes, as evidenced by an increase in the concentration of products of this process and the activity of the body’s antioxidant protection enzyme catalase. Assessment of the effect of Astragalus vulpinus herb extract on the free radical potential in the hypothalamic region against the background of the action of lipopolysaccharide-induced immune stress showed that the studied extract exhibits pronounced stress-protective and antioxidant properties characterized by changing the intensity of lipid peroxidation processes and by increase of catalase activity in the tissue of the hypothalamic brain region. Conclusions. Astragalus vulpinus herb extract has been proven to exhibit antioxidant effects by restoring activity of free radical processes.

The purpose of this study was to analyze the potential of tomato extract (Lycopersicum esculentum L.) in increasing the Sertoli and spermatogenic cells count of the contralateral testis in white rats (Rattus novergicus) with unilateral torsion. A total of 24 male Wistar white rats, aged 3-4 months old with a weight of 180-200 g were used in this study. The rat samples were randomly divided into 4 treatment groups: Group 1 as a negative control (NC) which consisted of rats without testicular torsion and given tomato extract; Group 2 as a positive control (PC) which consisted of rats with unilateral testicular torsion 720° for 6 hours without given tomato extract; Group 3 as treatment 1 (T1) consisted of rats that were subjected to unilateral testicular torsion 720o for 6 hours and were given tomato extract at a dose of 100 mg/kg BW/day; and Group 4 as treatment 2 (T2) consisted of rats that were subjected to unilateral testicular torsion 720° for 6 hours and were given tomato extract at a dose of 200 mg/kg BW/day. Each group consisted of six rats. Tomato extract was administered orally for 30 days. The observations of the number of Sertoli cells and spermatogenic cells (spermatogonia cells, spermatocytes, and spermatids) were carried out histologically with Hematoxylin-Eosin (HE) staining and the slides were examined with a light microscope. The data obtained was analyzed by ANOVA and continued with Duncan's test. The results showed that the administration of tomato extract significantly increased (P0.05) the number of Sertoli cells and spermatogenic cells of the contralateral testicular seminiferous tubule. Giving tomato extract 100 mg/kg BW/day resulted in an increase in the number of Sertoli cells and better spermatogenic cells than tomato extract 200 mg/kg BW/day. In conclusion the administration of tomato extract after detorsion can increase the number of Sertoli cells and spermatogenic cells of the contralateral testicular seminiferous tubules in white rats subjected to unilateral torsion of 720° for 6 hours

Analysis of a large number of works in recent decades on the effects of smoking on the body of a healthy person, shows that there is no organ or system in the body on which smoking would not have a harmful effect. Smoking is an aggressive risk factor for the occurrence and progression of groups of diseases, including lung cancer, coronary heart disease, hypertension, osteoporosis and some others. However, until recently, there was a small amount of experimental work in the literature to study the effects of tobacco smoke on the body in terms of age, including oxidative and inflammatory processes, permeability of cell membranes and endogenous intoxication, which would help find new reasonable ways to correct. The aim of the study was to determine the effect of tobacco smoke on the development of inflammation and permeability of cell membranes in rats of different ages in tobacco intoxication. Materials and methods. The experiments were performed on white male rats kept on the standard diet of the TSMU vivarium. Rats were divided into three age groups: immature, mature and senile. The model of dependence on the chronic effects of tobacco smoke was created using a sealed chamber. Tobacco smoke, formed from the burning of 6 Prima Silver (blue) cigarettes containing 0.6 mg of nicotine and 8 mg of tar, was fed into the chamber through openings. After 15, 30 and 45 days from the beginning of the defeat of the animals with tobacco smoke, they were removed from the experiment. In serum and tissue levels of C-reactive protein, alkaline phosphatase and gamma-glutamyltranspeptidase activity were determined. Results. In rats of different ages, an increase in serum C-reactive protein was observed during 45 days of tobacco smoke poisoning. The most sensitive to the action of the toxicant were senile rats, in which this indicator progressively increased and by the end of the experiment exceeded the level of the control group by 1.4 times. In addition, in animals of all ages (immature, mature and senile) there was an increase in alkaline phosphatase activity in serum and its decrease in the liver, which confirms the development of cholestasis and membrane-destructive processes in the liver of toxic animals. The latter indicates an increase in the activity in the serum of the membrane-dependent enzyme gamma-glutamyltranspeptidase throughout the experiment. By the end of the study, it had increased 1.9-fold in immature rats, 1.35-fold in mature rats, and 1.6-fold in senile rats. The most pronounced changes in cell membrane permeability were observed in immature animals. Conclusions. In senile rats, inflammatory processes progressed most markedly, as evidenced by the most pronounced increase in serum C-reactive protein. In immature rats, a progressive increase in the activity of membrane-dependent enzymes (gamma-glutamyltranspeptidase and alkaline phosphatase) in the serum after tobacco smoke poisoning, which by the end of the experiment significantly exceeded the level of rats of adult and senile age. This suggests that immature rats are more sensitive to exposure to tobacco smoke

The purpose of the research is to study the cumulative effect and subchronic toxicity of supramolecular complex of fenbendazole (SMCF).Materials and methods. The cumulative effect of SMCF was studied on 20 outbred white rats weighing 180 g that were divided into experimental and control groups of 10 animals each. SMCF was administered intragastrically to rats of the experimental groups for 24 days at the dose of 2000 mg/kg (1/10 of the previously established single LD50 (20000 mg/kg)) on the first day, then the dose was increased by 1.5 times every four day. The main criterion for evaluation of the results was the death of animals; we also observed the overall condition and behavior of rats. Subchronic toxicity was studied on 40 male white rats weighing 220–240 g. The animals were divided into 4 equal groups. The drug was administered daily to rats of the 1st, 2nd and 3rd experimental groups intragastrically using the doses of 1/5 (4000 mg/kg), 1/10 (2000 mg/ kg) and 1/20 (1000 mg/kg) of LD50 (20000 mg/kg) respectively for 7 days. The control group received 1% starch paste in an appropriate volume throughout the experiment. During the experiment, we observed physical signs and behavior of animals, food and water consumption; weight gains of animals on the 1st, 3rd, 5th and 7th days of the experiment. After the drug administration, animals were sacrificed by cervical dislocation. The laboratory examinations for hematological parameters and biochemical parameters were performed after treatment. Samples of organs (liver, heart, lungs, kidneys, spleen) were taken during necropsy to calculate mass coefficients and to make macroscopic examinations.Results and discussion. We found that the SMCF did not have any cumulative properties. The overall condition and behavior of rats was within the physiological range; animals consumed food and water and showed no signs of intoxication after oral administration of SMCF at doses of 1/5, 1/10 and 1/20 of LD50 for 7 days. The drug did not have influence on weight gains of animals. Hematological and biochemical parameters did not undergo significant changes and were comparable with the parameters of the control group. The mass coefficients of the internal organs did not differ statistically in experimental and control groups of rats.

In white rats with experimental hypothyroidism, changes in the myeloid compartment of the blood system induced by 6-h immobilization stress and the corrective effect of the analogue of leu-enkephalin (dalargin) on these shifts was analyzed. It was found that in rats with hypothyroidism, stress in the anxiety stage did not cause leukocytosis typical of euthyroid animals, but at the stage of resistance provoked leukopenia at the expense of eosinopenia and neutropenia with depletion of the intramedullary reserve. Dalargin increased white blood cells count, neutrophil count, and the intramedullary depot of these cells. [ABSTRACT FROM AUTHOR]

The study of pharmacological studies of the veterinary medicinal product for external use in the form of ointment - "Dibutalastin Ointment" is a mandatory stage of preclinical research of the drug, which is a new development of PP "Biopharm" and LLC "DEVIE". In a scientific experiment on laboratory animals, it is possible to assess the drug's safety for different terms of use to determine the zone of toxic action and doses that do not cause harmful effects on health. Therefore, the work aimed to carry out pharmacological studies of the drug with methyl salicylate and dimethyl sulfoxide in the form of an ointment for treating European fallow deer, deer, dogs, and cats with mastitis, arthritis, myositis, and injuries of various origins. The toxicological characteristics of the studied ointment "Dibutalastin" based on methyl salicylate and dimethyl sulfoxide were studied in an "acute" study on warmblooded animals. The average lethal dose (LD50) for intragastric administration could not be established, as the volume exceeded the permissible level. It was established that according to the "average lethal dose when entering the stomach" indicator, the veterinary medicinal product belongs to relatively non-toxic substances (toxicity class IV). The results of pharmacological studies indicate the absence of resorptive-toxic effects, sensitizing properties, and irritating effects on the mucous membranes of the eyes and skin. Pathomorphological studies of animal organs when studying the acute toxicity of the drug showed that the developed ointment with methyl salicylate and dimethyl sulfoxide does not cause pathological changes in internal organs. From the point of view of veterinary toxicology, this drug is safe. Further studies will be the next stage of pre-registration tests aimed at studying the embryotoxic effect of "Dibutalastin Ointment", which is mandatory material of the "Safety and residue studies" section of the dossier for this medicinal product. [ABSTRACT FROM AUTHOR]

The purpose of the research is conducting pharmaco-toxicological assessment of Gelmintal Mini Syrup based on a combination of moxidectin and toltrazuril. Materials and methods. The study was carried out on 64 white male rats and 40 white mice. Each animal species was divided into 4 equivalent groups of 6–10 animals each. When studying acute toxicity, the drug was administered in the form of a syrup (without dilution) to animals once at doses of 11 400, 22 800 and 28 500 mg/kg; when studying subchronic toxicity, the drug was administered daily for 14 days at doses of 570, 1425 and 2850 mg/kg (1/10 and 1/20 and 1/50 of the maximum possible dose administered into the stomach according to the vivisection results). When studying acute toxicity, the animals were followed up for 14 days; we recorded the general condition and behavior of the animals, changes in their body weight, the manifestation of toxic symptoms or possible death. When studying subchronic toxicity, the animals were followed up during the entire period of the drug use (14 days); on the 15th and 24th days of the experiment, we performed euthanasia, collected the blood to determine hematological and biochemical values, and carried out macroscopic examination of the organs. Results and discussion. We did not record the animals’ death at doses of 11 400, 22 800 and 28 500 mg/kg; and no signs of intoxication were noted for the entire follow-up period. LD50 of Gelmintal Mini Syrup exceeds the dose of 28500 mg/kg, i.e. the drug is classified as the 4th hazard class. The results of the studied subchronic toxicity showed that the drug was inactive at doses of 570 mg/kg, 1425 and 2850 mg/kg when administered orally for 14 days.

This study was conducted to evaluate physical properties and nutritive values of white rats (Rattus norvegicus) diets using different screen sizes of ground corn and different die sizes of the pellet. The experiment was divided into two trials. The first experiment aimed to find the effect of different particles of corn and die sizes of pellet on the physical properties and quality of white rat diet. The experiment consisted of 4 treatments and 4 replications namely, P1 (screen 3 mm and die 4 mm), P2 (screen 3 mm and die 16 mm), P3 (screen 5 mm and die 4 mm), and P4 (screen 5 mm and die 16 mm). The parameters measured were Pellet Durability Index (PDI), Specific density, Loose Bulk Density, and compacted Bulk Density of the pelleted diet. The second experiment was feeding trial to measure the performance of the white rats fed the pelleted diets. A total number of 20 white rats were used and divided into four treatments of the first experiment and replicated 5 times. The parameter measured were feed intake, final body weight, as well as the digestibility of dry matter and organic matter. Result of the experiments showed that the pellet durability index was significantly affected by the particle size of corn and the diameter of the pellet (P

According to the literature, different mechanisms and kinetics proceeding of regenerative growth has been established using the basic models of liver regeneration (after resection or chemically induced). Hence, in order to determine general regularities of the adaptive-compensatory processes in various pathological conditions, the processes taking place in the cholestatic liver of adult white rats during the first four days after common bile duct ligation have been studied. It has been shown that in cholestatic liver, compensatory-adaptive processes take place with different kinetics compared to those after resection. In particular, in response to the increased functional load caused by destructive processes during cholestasis, the liver, at an early stage, responds by simple division of high ploidy (binuclear tetraploid) cells and further provides their quantitative increase. The difference between the processes taking place in cholestatic and resected liver is more expressed on the third and fourth day after common bile duct ligation. In particular, 4c cells are still highest in cholestatic liver, while all ploidy cells are present in equal numbers in the regenerated liver after resection. This fact of compensatory growth characteristic for reparative regeneration was not detected in cholestatic liver at the mentioned date. [ABSTRACT FROM AUTHOR]

The purpose of the research is to study the cumulative properties of the supramolecular complex of ivermectin.Materials and methods. Studies were conducted in the vivarium of the VNIIGIS named after K. I. Skrjabin. For the purpose of the experiment, 20 outbred white rats weighing 180 g were selected, which were divided into experimental and control groups of 10 animals each. To determine the cumulation coefficient, we used a method taking into account the death of animals upon repeated administration of the drug — a test of subchronic toxicity in accordance with the “Guidelines for the experimental (preclinical) study of new pharmacological substances”. For the rats in the experimental group the drug was administered orally during the first four days at a dose of 35.5 mg/kg equal to 1/10 of the previously established single LD50 (298.0 mg/kg). Then, the dose was increased by 1.5 times every next four days. The rats in the control group were injected with 2 ml of water. The experiment was carried out for 28 days. Throughout the experiment, animals were monitored, considering their condition and degree of activity.Results and discussion. According to the accepted classification, the supramolecular complex of ivermectin belongs to the group of substances with weakly expressed cumulative properties, since the cumulation coefficient is 6.05 (IV toxicity group).

The purpose of the research is to assess the subchronic toxicity of the complex drug for dogs and cats “Inspector Kvadro” for cutaneous use.Materials and methods. The experiment was carried out on 40 male rats with an initial body weight of 220–240 g. The animals were divided into 4 equivalent groups of 10 rats each. The drug was applied daily for 7 days on a clipped skin in the back at doses of 1080; 540 and 216 mg/kg. During the experiment, we observed the general condition and behavior of animals, possible death, intake of food and water, visible physiological functions, the condition of the coat and skin at the site of application of the drug in order to evaluate the irritating effect. On the first day and 10 days after the last use of the drug, the animals were euthanized and blood samples were taken to determine hematological and biochemical parameters, a macroscopic examination of the organs was carried out, and the functional state of the central nervous system was evaluated.Results and discussion. Daily application on the skin of the drug in test doses did not lead to a change in the general condition and behavior of rats. Experienced animals normally took food and water, adequately responded to external stimuli. However, the recorded changes in some indicators indicate damage to the hepatocytes and indicate insufficient liver function. Given the increased relative liver mass of the animals of the first experimental group and the change in biochemical parameters, we can conclude that the drug has a pronounced hepatotoxic effect at a dose of 1080 mg/kg for cutaneous use for 7 days. Accordingly, the dose of 1080 mg/kg is toxic, the doses of 540 and 216 mg/kg are threshold.

The carried out research revealed negative impact of chronic T-2 toxin intoxication on immune status of white rats vaccinated against Salmonellosis. This is illustrated by erythropoiesis inhibition and amenia development. The immunized animals and animals chronically exposed to T-2 toxin typically demonstrate a decrease in the number of erythrocytes by 13% and in the level of hemoglobin by 9.6% due to reduced hemolotic action of xenobiotics and hematopoiesis depression. The use of aminoseleton in the vaccination scheme enhances adaptive immunity and natural resistance of the organism under these conditions. On day 30 post vaccination the vaccinated rats demonstrated an increase (in comparison to the control animals) in the number of erythrocytes - by 14.1%; hemoglobin - by 11.7%, number of leukocytes - by 6.5%, absolute number of segmentonuclear neutrophils - by 23%, immature neutrophils - by 11.1%. It is related to active formation of neutrophils in the marrow and their further migration to the blood circulation system for phagocytosis. The detected morphological changes in blood of the vaccinated and exposed animals suggest hematological disorder associated with T-2 toxicosis demonstrated as erythropoiesis inhibition and leucopenia. Decrease in specific humoral immunity and natural resistance caused by chronic T-2 toxicosis following experimental infection with Salmonella cholerae suis had a negative effect on the animal lifetime and safety.

The article is devoted to the study of the influence of arsenic mineral water (MW) with its internal application to the organism of healthy white rats. MW does not have toxic effects and does not cause pathological changes in the organism of animals. There was established a slight sedative effect on the functional state of the central nervous system and emotional activity of animals, a significant stimulating effect on the functional state of the kidneys and a moderate stimulating effect on the bile-excretory function of the liver. A decrease in the activity of aerobic oxidation in the tissues of the studied organs (stomach, liver, kidneys) was detected. Directions for further experimental studies are formulated.

Neem (Azadirachta indica A. Juss) is one of the traditional medicines used by local people as antioxidants, antihyperlipidemia, anti-inflammatory, and treatment for other diseases such as heart disease. This study aimed to analyze the effect of neem leaf ethanolic extract on aortic wall thickness and aortic lumen diameter of white rats induced by high-fat feed. The male Wistar rats (Rattus norvegicus L.) with 2 months of age were used. This research was an experimental study with a Randomized Complete Block Design consisted of 6 treatments (P0: commercial feed, P1: high-fat feed and duck egg yolk 2.5 ml/200 g BW per oral, P2: P1 + 8 mg/200 g BW simvastatin, P3-P5: P1+ neem leaf extract of 75, 100, and 125 mg/200 g BW respectively) and 4 replications. Aortic preparations were made by the paraffin method and Hematoxylin-Eosin staining. Data were analyzed using ANOVA followed by Duncan Multiple Range Test with 95% confidence level. The result showed that the decrease in wall thickening and lumen narrowing was getting higher along with the increasing doses of ethanol extract of neem leaves. Based on the result of this research, it was found that the high doses of neem leaf ethanolic extract has the same ability with simvastatin to reduce aortic wall thickning and aortic lumen narrowing of white rats. This study is give a new information that the neem leaves can be used as an alternative medicine for cardiovascular disease. [ABSTRACT FROM AUTHOR]

In this study we tried to determine possible roles of Ca2+ on hepatocytes division. Calcium have been demonstrated to be vital mediators. The period of cell cycle showed too effected by calcium. In the this paper we tried to report the relation between calcium concentration and the cellular proliferation ratio for hepatocytes of white rats we divided the experimental animals into four groups each group contains five experimental animals at the same age and weight, the results of this study showed that the cellular proliferation ratio increased with the calcium concentration increasing, where the ratio of hepatic division progressed to get 35.33±0.33C at concentration 20mg/ml, 55.33±0.33B at concentration 40mg/ml and 70.33±0.33A at concentration from Ca2+. [ABSTRACT FROM AUTHOR]

In this study we tried to determine possible roles of Ca2+ on hepatocytes division. Calcium have been demonstrated to be vital mediators. The period of cell cycle showed too effected by calcium. In the this paper we tried to report the relation between calcium concentration and the cellular proliferation ratio for hepatocytes of white rats we divided the experimental animals into four groups each group contains five experimental animals at the same age and weight, the results of this study showed that the cellular proliferation ratio increased with the calcium concentration increasing, where the ratio of hepatic division progressed to get 35.33±0.33C at concentration 20mg/ml, 55.33±0.33B at concentration 40mg/ml and 70.33±0.33A at concentration from Ca2+. [ABSTRACT FROM AUTHOR]

The aim of this study was to determine the effects of antiepileptic drugs (AEDs) on the sperm characteristic and some reproductive parameters in male rats. In this experiment (60) animals of male rats were divided randomly into four equal groups, Group 1: rats were treated with normal saline (0.9% NaCl) and considered as control group. Group 2: rats were treated with Phenytoin (100 mg/kg) body weight. Group 3: rats were treated with Topiramate (100 mg/kg) body weight. Group 4: rats were treated with Levetiracetam (150 mg/kg) body weight. The results showed a significant decrease (P <0.05) in testicular weight, total epididymal weight, prostate weight and seminal vesicle weight in G2, G3 group compared with control group. There was a significant decrease (p≤0.05) in serum testosterone level in all groups as compared to the control group, with no significant difference in serum FSH level and serum LH level in all groups as compared to the control group. These results show a significant (p<0.05) decrease in the sperm count, active Sperm motility %, Viable sperm % in each treated group in comparison with control group. On the other hand, there was a significant (p<0.05) increase in abnormal sperms % in each treated group compared with control group. [ABSTRACT FROM AUTHOR]

Aim: Aim of this study was to see the immunopathological changes against the microfilariae (Mf) and adult worms of a bovine filarid, Setaria cervi in the tissues of vital organs of experimentally infected white rats. The effect of diethylcarbamazine (DEC) was also observed on the Mf, as leukocytes especially lymphocytes produce immunoglobulins which opsonize and increase the efficacy of DEC against circulating Mf. Effect of this drug was also assessed on liver enzymes in the microfilaremic rats. Materials and Methods: Microfilaremia was established by implanting adult worms intraperitoneally and by the infusion of Mf recovered from the uterus of gravid female worms. DEC was administered orally for six consecutive days in the rats having patent infection. Differential leukocyte count was recorded every 3rd day, and liver enzymes were estimated every 10th day in both treated and untreated rats. Pathological changes were observed in HE stained sections of vital organs where Mf or adult worms were trapped. Results: Destruction and reduction in microfilarial density were noticed in microfilaremic rats treated with DEC. Trapped Mf and embedded worms revealed heavy cellular infiltrations by defensive cells which surrounded and attached with the body surface of the Mf as well as adult worms for their destruction and piece meal clearance. Immune-mediated pathology was observed in the tissue sections of lungs, spleen, and liver. Liver enzymes were elevated during the period of higher parasitemia. Conclusion: There was a moderate level of immunopathology against the Mf and adult worms by the leukocytes in experimentally infected microfilaremic rats. Mf were in the process of degeneration where they got trapped. Moderate increase in liver enzyme was noticed which was slightly more in untreated group. Although a fraction of Mf gets killed in the peritoneum, majority of them successfully enter the systemic circulation and survive for about 54 days, which is sufficient enough for conducting immunological and chemotherapeutic studies.

Objective: Aim: The aim of the study was to investigate the activity of bioenergetic processes in rats under conditions of simultaneous exposure to malathion and carbon tetrachloride and after the use of enterosgel., Patients and Methods: Materials and Methods: Experiments were conducted on rats. The rats were divided into nine groups.Malathion was administered daily (for 30 days) at a dose of 20 mg / kg body weight of the animal. Tetrachloromethane was administered twice (every other day) as a 50% oil solution at a dose of 1.0 ml / kg body weight. The intensity of energy supply processes was assessed by the activity of succinate dehydrogenase and cytochrome oxidase, impaired carbohydrate metabolism in terms of glucose and glycogen., Results: Results: It was noted that succinate dehydrogenase activity in the liver decreased 2 times, in the myocardium - 1.6 times. On the thirty and seventh day of administration of toxicants after enterosorbent use, succinate dehydrogenase activity increased in the liver by 20%, cytochrome oxidase by 27%, in the myocardium - by 31% and 23%, respectively. The content of glucose in the serum after exposure to toxicants increased maximally (2.4 times) at the end of the study. In contrast, the glycogen content in the liver decreased by 48%, in the myocardium by 13%. The use of enterosgel resulted in a decrease in serum glucose., Conclusion: Conclusions: The use of enterosgel leads to the restoration of energy processes in the body of affected rats, which is confirmed by increased activity of mitochondrial enzymes, lowering glucose and increasing glycogen in the studied organs.

The aim of the research was to study the effect of the complex antimicrobial drug Triolact on blood's morphological and biochemical parameters in white rats in a subacute toxicity experiment. It was shown that threshold dose was 500.0 mg/kg, while highest no-effect dose was 100.0 mg/kg. Triolact did not have a negative effect on the clinical status, biochemistry, or blood cell morphology after 14 days of repeated intragastric administration of 100.0 mg/kg or 250.0 mg/kg either during the application of the drug or in the recovery period. The changes caused by the dose of 500.0 mg/kg were reversible. Increase in α-globulin, creatinine, and urea levels were 6.9, 22.9, and 15.1% respectively, but these indicators in test animals returned to normal within the 10 days of the recovery period. It can be concluded that the determination of the toxic dose in the performed experiments failed with consideration for the morphometric analysis, macro- and microscopic examination of organs, and morphology and biochemistry of blood. [ABSTRACT FROM AUTHOR]

Obat Pahit from Lingga Malay ethnic is a traditional medicine which has believed by the local people to maintain the body stamina (immunomodulators and antioxidants). This study aimed to investigate the effect of Obat Pahit Potion on kidney histological structure of white male rats (Rattus norvegicus). This research was an experimental study with a Randomized Complete Block Design consisted of 15 treatments and 3 replications. The experiment groups consisted of control groups (Stimuno, distilled water, and CMC-Na 1%) and three treatment groups of Obat Pahit Potion (Kalan, SP4, Linau) with 4 different dosages. The samples of a kidney of white rats were prepared for histological observation using paraffin method and Hematoxylin-Eosin staining. The results of this study showed that three kinds of Obat Pahit with four different dosages showed the expansion of glomerular cells on kidney tissue. Damage that occurs in the kidneys is still in small amounts and normal range which is less than 25%. The results of this study provide information for the society that the consumption of Obat Pahit Potion will not cause toxicity effect on the kidney. [ABSTRACT FROM AUTHOR]

The contribution of prenatal hypoxic damage to the CNS to the formation of high sensitivity of the body to lead acetate was studied. Prenatal fetal hypoxia was modeled by the administration of sodium nitrite in doses of 5, 25, and 50 mg/kg to pregnant female rats. Cognitive capacities of mature offspring were evaluated in the radial maze test and Morris water maze test. After attaining learning criterion in the radial maze, lead acetate in a dose of 80 mg/kg was added to the drinking water of all animals over 2 weeks. Testing was performed during the exposure to the agent until disruption of the conditioned behavior. It was found that severe prenatal hypoxia (induced by the administration of 50 mg/kg sodium nitrite) impaired spatial memory, increased latency of funding the platform in Morris water maze test, and serves as a factor contributing to earlier manifestations of the neurotoxic effects of lead acetate. [ABSTRACT FROM AUTHOR]

The synthesis of a water-soluble polymer nanocomposite with Ag(0) particles encapsulated in a poly(1-vinyl-1,2,4-triazole) matrix is reported. The nanocomposite contains isolated spherical Ag(0) particles sized from 2 to 8 nm that are uniformly distributed in the polymer matrix and have a characteristic plasmonic absorption maximum at 412 nm. The new nanocomposite belongs to marginally hazardous category 4. The structural morphology of animal brain tissue is not disturbed after subacute intragastric injection. The bacteriostatic concentration of the nanocomposite varies in the range 0.5 - 32 μg/mL; bactericidal, 1 - 64 μg/mL. [ABSTRACT FROM AUTHOR]

Objective of research: studying of acute toxicity of supramolecular complexes of Fenbendazole developed using chemical mechanical technology in comparison with the substance of preparation injected to the stomach of white mice and rats. Materials and methods: The research of parameters of acute toxicity of the supramolecular complexes of Fenbendazole was conducted according to «Guidelines for experimental (pre-clinical) studies of new pharmaceutical compounds», and other rules. Preparations were injected to the stomach of white rats and mice of both gender with the body mass 160–180 and 18–20 g. at a single dose 7 000, 10 000, 15 000 and 20 000 mg/ kg, respectively. Each dose was investigated on 6 white rats and 10 white mice. Within 14 days we observed the general health status and behavior of animals, symptoms of intoxication and eventual death. We also conducted postmortem examinations of the fallen animals. The parameters of acute toxicity were determined by the method of Litchfield J. and Wilcoxon F. (M.D. Belen’ky, 1963). Results and discussion: It was not possible to detect LD50 in supramolecular complex of Fenbendazole and the base drug Fenbendazole due to the low toxicity. The maximum endurable dose of supramolecular complex no. 2 was 10 000 mg/kg, LD50 -value in supramolecular complex of Fenbendazole no. 2 at injection to the stomach of white mice and rats was 20 000 mg/kg.

Prazifen in a dose of 1,0/10,0 and 3,0/30,0 mg/kg DV respectively on praziquantel and fenbendazole at introduction in stomach during the various periods of embryogenesis didn't show embryotoxic and teratogenic action.

Subacute toxicity of salt briquettes with ivermeсtin is studied. The drug has no influence on hematologic and biochemical parameters, doesn't cause pathological changes in tissues and bodies. The body weight and weight of internal organs of rats were as in control groups. The preparation doesn't have cumulative action.

The present study was undertaken to know the reproductive toxicity of mercury chloride in male and females of white rats. This study was used twelve (6 male and 6 females) white rats of approximately of the same body weight (200-220 g) divided equally in to 3 groups; the first group (T1) was received mercury chloride (1mg/kg B.W intraperitoneally once daily for 30 days). While the second group (T2) was received, mercury chloride (1.5mg/kg B.W intraperitoneally once daily for 30 days).Third group was received only0.2ml of Distilled water considered as control group. At the end of experiment, the animals were sacrificed and small pieces of (2cm3) were taken from ovary, uterus and testis of all animals to histopathology. Histopathological sections of these organs of (T2) group was showed severe pathological changes characterized by vaculation in epithelial cells of uterus, cystic dilatation of uterine glands with degeneration of epithelial lining of its ,hemorrhage and decrease in number of growing follicles in ovary also there were sever pathological changes in the testes. While (T1) group was showed less pathological changes characterized by hyperplasia of epithelial lining with few and small uterine gland in uterus, presence of large secondary follicles in ovary and there were less pathological changes in testes. [ABSTRACT FROM AUTHOR]

The influence of 1 % solution of cyfluthrin on reproductive function of rats is studied. The drug in a dose of 1/5 from LD50 during 10 weeks didn’t influence on fecundity, reproductive function and postnatal development of posterity.

Aim: Aim of this study was to see the immunopathological changes against the microfilariae (Mf) and adult worms of a bovine filarid, Setaria cervi in the tissues of vital organs of experimentally infected white rats. The effect of diethylcarbamazine (DEC) was also observed on the Mf, as leukocytes especially lymphocytes produce immunoglobulins which opsonize and increase the efficacy of DEC against circulating Mf. Effect of this drug was also assessed on liver enzymes in the microfilaremic rats. Materials and Methods: Microfilaremia was established by implanting adult worms intraperitoneally and by the infusion of Mf recovered from the uterus of gravid female worms. DEC was administered orally for six consecutive days in the rats having patent infection. Differential leukocyte count was recorded every 3rd day, and liver enzymes were estimated every 103th day in both treated and untreated rats. Pathological changes were observed in HE stained sections of vital organs where Mf or adult worms were trapped. Results: Destruction and reduction in microfilarial density were noticed in microfilaremic rats treated with DEC. Trapped Mf and embedded worms revealed heavy cellular infiltrations by defensive cells which surrounded and attached with the body surface of the Mf as well as adult worms for their destruction and piece meal clearance. Immune-mediated pathology was observed in the tissue sections of lungs, spleen, and liver. Liver enzymes were elevated during the period of higher parasitemia. Conclusions: There was a moderate level of immunopathology against the Mf and adult worms by the leukocytes in experimentally infected microfilaremic rats. Mf were in the process of degeneration where they got trapped. Moderate increase in liver enzyme was noticed which was slightly more in untreated group. Although a fraction of Mf gets killed in the peritoneum, majority of them successfully enter the systemic circulation and survive for about 54 days, which is sufficient enough for conducting immunological and chemotherapeutic studies. [ABSTRACT FROM AUTHOR]

The article highlights the changes in weight of thyroid gland of albino rats after administration of mesenchymal stem cells eksperimentalnogo hypothyroidism. The aim of this study was to investigate the effect of stem cells on the change in weight of the thyroid gland after treatment of mesenchymal stem cells. There was a question to study the effect of mesenchymal stem cells in regenerative processes in the thyroid gland in white rats and to study the effect of MSCS on the weight of the thyroid gland in animals after experimental hypothyroidism. The results of the research give an opportunity to analyze and further study the effect of mesenchymal stem cells on thyroid insufficiency in varieties, as well as study the development of reparative processes in the tissue of the thyroid gland. [ABSTRACT FROM AUTHOR]

The production vibration is known to be a powerful stressor which may induce the complex of disorders leading to the vibration-induced disease development. Along with the appearance of the clinical syndromes the neurosis-like, the psychoemotional disorders, the ipochondric, asthenic and asthenovegetative syndromes, the diffuse disorders of the bioelectrical brain activity with the participation of the cortex of the large hemispheres as well as the stem and diencephalic brain structures were observed to occur in the patients with the vibration-induced disease. However, at present there are no clear arguments in favour that namely the exposure to vibration may promote the occurrence of the neurotic disorders, the neurosis-like disorders of psychic activity, the disadaptation signs, the emotional disorders as well as the pathological changes in the bioelectrical brain activity in exposure to vibration. This work aimed to reveal the changes in the central nervous system (CNS) based on the study results of the behavior and bioelectrical brain activity in the animals. The experimental studies were performed using the mature inbreeding rats males. The chronic experiment was performed by means of the modificated vibrostend VEDS-10a, the vibration levels taken corresponded to the exposure in reality to vibration under production conditions. The studies were performed based on 4 study series: for 15 days, one, two, and four months of exposure to vibration. The intact rats were used as the control ones. The animal behavior responses with assessing the motor activity, the orientating and learning as well as the emotional behavior components were observed in the «юрen field». Recording and processing the electroencephalographic data were performed using the electroencephalograph Neuron-spectrum 4 (Joint-Stock Company «Neurosoft», Russia). The information was processed using the standard methods of the variation statistica, statistical processing the data was performed by means of the Packet of Applied Programs EXCEL Packet Office 2003 (in OS «W1NDOWS Xp»), «Statistica for windows - Version 6». The study of the behavior activity in the animals has shown that the increase in the common motor activity, the nonspecific character, the high emotional strain of the negative character, the negative emotional state were found to occur in the rats exposed to vibration, thereto, the orientating and learning activity was at the previous level. The change in the bioelectrical brain activity was characterized by the increase in the slow-wave activity of the delta-range after one month of exposure to vibration and the latent period prolongation of the visual induced potentials (V1P) after 15 days of exposure to vibration.

In the materials there are presented, results of the synthesis and biological testing of the new nanocomposite which, contains silver nanoparticles incapsulated. in the synthetic polymer poly-1-vinyl-1,2,4-triazole. The synthesis method, of silvercontaining polymere nanocomposite is shown. With the use of modern equipment evidence of the nanocomposite with the size of 2-10 nm silver production, are substinated. It was revealed, that the new nanocomposite causes slight changes in the tissue structure of experimental animals internal organs and biochemical shift that is characteristic for the compensatory-adaptive reactions development. There are presented, the results of the silver nanoparticles distribution, mainly in the kidney and. liver tissue.

There are age-dependent anatomical structure of white rats' hypophysis, taking with biometric methods are described in the article. These features are analyzed on correlation with reproductive status, conformities of hypophysis' growth and studying of hypophysis' lobe relative area in females and males, that has the applied value for further experimental works.