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WOS:000384908900004 Water hyssop (Bacopa monnieri (L.) Pennell) is a medicinal plants. Its upper and lower halves of leaf explants were incubated in Murashige and Skoog (MS) medium supplemented with 0.25, 0.50 and 1.0 mg/L benzylaminopurine (BA) for 8 weeks; the explants were exposed to white (W) and red and blue (R and B, respectively) light-emitting diodes (LEDs), at 4: 1, 3: 1, 2: 1 and 1: 1 R and B light ratios, respectively. Shoot regeneration (100%) was achieved from all explants at all applied concentrations of BA and LED types. All explants showed different BA concentration requirements for regeneration of the maximum number of shoots. Longer shoots were obtained on medium with 0.25 mg/L BA. The W LED lighting system was found to be more effective for regenerating the maximum number of shoots (26.11) per explant (on the upper half of the leaf). Conversely, longer and shorter shoots were generated under 1: 1 R: B and W LEDs, respectively. The number of shoots per explant ranged from 9.67-24.0 (full leaf), 6.33-25.92 (lower half of the leaf) and 7.33-27.33 (upper half of the leaf), respectively, in response to BA and LED light. Shoot length ranged from 0.94-1.90 cm (full lamina), 0.70-2.11 cm (lower half of the leaf) and 0.93-1.83 cm (upper half of the lamina) in response to BA and LED lifght. Regenerated shoots were successfully rooted using indole-3-butyric acid (IBA) and acclimatized in the aquarium provided with tap water. Scientific Research Project Commission (BAP) of Karamanoglu Mehmetbey University [03-M-14] The authors thank the Scientific Research Project Commission (BAP) of Karamanoglu Mehmetbey University for funding the project number 03-M-14.

WOS:000429181000001 Efficacy of different Light Emitting Diodes (LEDs) on in vitro whole plant regeneration of water hyssop (Bacopa monnieri L. Pennel) was investigated in this study. Shoot tip explants were cultured on MS medium with BA (0.25, 0.50 and 1.0 mg l(-1)) and incubated under Red: Blue (R:B) LEDs in different combinations (4:1, 3:1, 2:1 and 1:1 R: B and white LEDs) for 10 weeks. Callus induction and shoot regeneration was noted on all explants irrespective of LEDs type or BA concentration. Shoots per explant and shoot length ranged 6.56-10.42 and 0.96-1.71cm respectively. Maximum number of 10.42 shoots with highest shoot length of 1.71 cm was achieved under 1:1 R:B LEDs. Whereas, different BA concentrations generated 4.97-11.98 shoots per explant with shoot length in the range of 0.93-1.35 cm. Maximum shoots per explant (10.35) with more longer shoots (1.31 cm) was achieved on MS medium containing 1.0 mg l(-1) BA. Comparing of BA x LEDs, maximum number of 15.17 shoots per explant was induced under 1.0 mg l(-1) BA x 2:1 R: B LEDs. Whereas, longer shoots (2.68 cm) were scored under 0.5 mg l(-1) BA x 2:1 R: B LEDs. Regenerated shoots were rooted using 1.0 mg l(-1) IBA in four weeks followed by acclimatization in the aquariums provided with tap water. Results revealed that LEDs in combination with BA can be successfully employed for multiple shoot regeneration of water hyssop.