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3 results on '"virulent mycobacterium-tuberculosis"'

1. Interaction between Intracellular Bacterial Pathogens and Host Cell Mitochondria

Author

Fabrizia Stavru, Anna Spier, Pascale Cossart, Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Ecole doctorale Bio Sorbonne Paris Cité (BioSPC) (ED 157), Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5), Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA)

Subjects
Cytoplasm, Physiology, [SDV]Life Sciences [q-bio], Mitochondrion, Mitochondrial Dynamics, eputhelial-cells, MESH: Animals, rickettsii infection, 0303 health sciences, Ecology, biology, Effector, 030302 biochemistry & molecular biology, apoptosis, MESH: Bacteria/pathogenicity, MESH: Mitochondria/ultrastructure, Mitochondria, Cell biology, MESH: Vacuoles/microbiology, Infectious Diseases, MESH: Cytoplasm/microbiology, Host-Pathogen Interactions, Intracellular, Microbiology (medical), virulent mycobacterium-tuberculosis, cytochrome-c release, 03 medical and health sciences, Immune system, nf-kappa-b, Genetics, Animals, Humans, legionella-pneymophila, 030304 developmental biology, mocecular parhogenesis, MESH: Humans, Bacteria, General Immunology and Microbiology, ehrlichia-chaffeensis, Intracellular parasite, MESH: Host-Pathogen Interactions, Cell Biology, biology.organism_classification, dependeny inhibition, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Mitochondrial Dynamics, Apoptosis, Vacuoles, MESH: Mitochondria/microbiology, Function (biology)
Abstract

Mitochondria are essential and highly dynamic organelles whose morphology is determined by a steady-state balance between fusion and fission. Mitochondrial morphology and function are tightly connected. Because they are involved in many important cellular processes, including energy production, cell-autonomous immunity, and apoptosis, mitochondria present an attractive target for pathogens. Here, we explore the relationship between host cell mitochondria and intracellular bacteria, with a focus on mitochondrial morphology and function, as well as apoptosis. Modulation of apoptosis can allow bacteria to establish their replicative niche or support bacterial dissemination. Furthermore, bacteria can manipulate mitochondrial morphology and function through secreted effector proteins and can also contribute to the establishment of a successful infection, e.g., by favoring access to nutrients and/or evasion of the immune system.

Published
2019
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2. PrimaTB STAT-PAK assay, a novel, rapid lateral-flow test for tuberculosis in nonhuman primates

Author

Larry Handt, Sean Ervin, Frank Ervin, Peter Szczerba, Konstantin P. Lyashchenko, Javan Esfandiari, Sherri L. Motzel, Peter J. Didier, Carol A. Nacy, John M. Pollock, Frank A. W. Verreck, Rena Greenwald, Marc V. Washington, James McNair, Jan A.M. Langermans, Candace Mccombs, Susan V. Gibson, David Greenwald, and Peter Andersen

Subjects
skin reactivity, diagnosis, Clinical Biochemistry, rhesus-monkeys, antibody-responses, bovis, Serology, 0403 veterinary science, calmette-guerin, Chlorocebus aethiops, Immunology and Allergy, Immunoassay, 0303 health sciences, medicine.diagnostic_test, 04 agricultural and veterinary sciences, Antibodies, Bacterial, 3. Good health, antigen recognition, ASG Infectieziekten, Microbiology (medical), Tuberculosis, 040301 veterinary sciences, Immunology, macaca-mulatta, virulent mycobacterium-tuberculosis, Biology, Sensitivity and Specificity, Lateral flow test, Mycobacterium tuberculosis, 03 medical and health sciences, Antigen, Tuberculosis diagnosis, Bacterial Proteins, serum antibodies, medicine, Animals, 030304 developmental biology, Antigens, Bacterial, Bacterial disease, Tuberculin Test, Primate Diseases, Membrane Proteins, medicine.disease, biology.organism_classification, Virology, Macaca mulatta, Macaca fascicularis, Microbial Immunology
Abstract

Tuberculosis (TB) is the most important zoonotic bacterial disease in nonhuman primates (NHP). The current diagnostic method, the intradermal palpebral tuberculin test, has serious shortcomings. We characterized antibody responses in NHP againstMycobacterium tuberculosisto identify immunodominant antigens and develop a rapid serodiagnostic test for TB. A total of 422 NHP were evaluated, including 243 rhesus (Macaca mulatta), 46 cynomolgus (Macaca fascicularis), and 133 African green (Cercopithecus aethiops sabaeus) monkeys at five collaborative centers. Of those, 50 monkeys of the three species were experimentally inoculated withM. tuberculosis. Antibody responses were monitored every 2 to 4 weeks for up to 8 months postinfection by MultiAntigen Print ImmunoAssay with a panel of 12 recombinant antigens. All of the infected monkeys produced antibodies at various levels and with different antigen recognition patterns. ESAT-6 and MPB83 were the most frequently recognized proteins during infection. A combination of selected antigens which detected antibodies in all of the infected monkeys was designed to develop the PrimaTB STAT-PAK assay by lateral-flow technology. Serological evaluation demonstrated high diagnostic sensitivity (90%) and specificity (99%). The highest rate of TB detection was achieved when the skin test was combined with the PrimaTB STAT-PAK kit. This novel immunoassay provides a simple, rapid, and accurate test for TB in NHP.

Published
2007

3. PrimaTB STAT-PAK Assay, a Novel, Rapid Lateral-Flow Test for Tuberculosis in Nonhuman Primates

Author

Lyashchenko, K.P., Greenwald, R., Esfandiari, J., Greenwald, D., Nacy, C.A., Gibson, S., Didier, P.J., Washington, M., Szczerba, P., Motzel, S., Handt, L., Pollock, J.M., McNair, J., Andersen, P., Langermans, J.A.M., Verreck, F., Ervin, S., Ervin, F., McCombs, C., Lyashchenko, K.P., Greenwald, R., Esfandiari, J., Greenwald, D., Nacy, C.A., Gibson, S., Didier, P.J., Washington, M., Szczerba, P., Motzel, S., Handt, L., Pollock, J.M., McNair, J., Andersen, P., Langermans, J.A.M., Verreck, F., Ervin, S., Ervin, F., and McCombs, C.

Abstract

Tuberculosis (TB) is the most important zoonotic bacterial disease in nonhuman primates (NHP). The current diagnostic method, the intradermal palpebral tuberculin test, has serious shortcomings. We characterized antibody responses in NHP against Mycobacterium tuberculosis to identify immunodominant antigens and develop a rapid serodiagnostic test for TB. A total of 422 NHP were evaluated, including 243 rhesus (Macaca mulatta), 46 cynomolgus (Macaca fascicularis), and 133 African green (Cercopithecus aethiops sabaeus) monkeys at five collaborative centers. Of those, 50 monkeys of the three species were experimentally inoculated with M. tuberculosis. Antibody responses were monitored every 2 to 4 weeks for up to 8 months postinfection by MultiAntigen Print ImmunoAssay with a panel of 12 recombinant antigens. All of the infected monkeys produced antibodies at various levels and with different antigen recognition patterns. ESAT-6 and MPB83 were the most frequently recognized proteins during infection. A combination of selected antigens which detected antibodies in all of the infected monkeys was designed to develop the PrimaTB STAT-PAK assay by lateral-flow technology. Serological evaluation demonstrated high diagnostic sensitivity (90%) and specificity (99%). The highest rate of TB detection was achieved when the skin test was combined with the PrimaTB STAT-PAK kit. This novel immunoassay provides a simple, rapid, and accurate test for TB in NHP.

Published
2007

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